Title:
METHOD FOR RAPIDLY EXTRACTING PHYCOCYANIN
Document Type and Number:
WIPO Patent Application WO/2023/015792
Kind Code:
A1
Abstract:
The present invention relates to the technical field of protein extraction, in particular to a method for rapidly extracting phycocyanin. The method comprises the following steps: mixing aquaculture water containing microalgae with a protein protectant, and performing a first centrifugation to obtain concentrated algae liquid; mixing the concentrated algae liquid with a cell cryoprotectant, and performing repeated freezing and thawing to obtain thawed concentrated algae liquid; mixing the thawed concentrated algae liquid with the protein protectant and a surfactant, and crushing cells to obtain crushed algae liquid; subjecting the crushed algae liquid to a second centrifugation to obtain supernatant; mixing the supernatant with ammonium sulfate and polypropylene glycol, and performing stirring; subjecting the stirred solution to a third centrifugation to obtain a precipitate; and washing the precipitate with an ethanol aqueous solution, performing a fourth centrifugation, and freeze-drying the precipitate. The extraction method of the present invention has simple and rapid operation and a low cost and the obtained phycocyanin has a high purity.
Inventors:
HUANG CHENGTAN (CN)
PAN JUN (CN)
YE LEI (CN)
HUANG MIN (CN)
PAN JUN (CN)
YE LEI (CN)
HUANG MIN (CN)
Application Number:
PCT/CN2021/137050
Publication Date:
February 16, 2023
Filing Date:
December 10, 2021
Export Citation:
Assignee:
HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD (CN)
International Classes:
C07K1/14; C07K1/30; C07K14/795
Domestic Patent References:
| WO2018033814A1 | 2018-02-22 |
Foreign References:
| CN104672325A | 2015-06-03 | |||
| CN113480638A | 2021-10-08 | |||
| CN109160947A | 2019-01-08 | |||
| IN201403981I1 |
Other References:
ZHAO JING, QI YAN; WANG YUE-HUA; FENG XU-QIAO: "Research on phycocyanin protein isolation and purification technology and influence factors", FOOD AND FERMENTATION INDUSTRIES, vol. 40, no. 10, 20 August 2014 (2014-08-20), pages 168 - 174, XP093034517, DOI: 10.13995/j.cnki.11-1802/ts.2014.10.068
LI YING, ZHONG ZHANG, MARIA PACIULLI, ALIREZA ABBASPOURRAD: "Extraction of phycocyanin-A natural blue colorant from dried spirulina biomass: Influence of processing parameters and extraction techniques", JOURNAL OF FOOD SCIENCE, vol. 85, no. 3, 30 January 2020 (2020-01-30), pages 727 - 735, XP093034520, DOI: 10.1111/1750-3841.14842
LI YING, ZHONG ZHANG, MARIA PACIULLI, ALIREZA ABBASPOURRAD: "Extraction of phycocyanin-A natural blue colorant from dried spirulina biomass: Influence of processing parameters and extraction techniques", JOURNAL OF FOOD SCIENCE, vol. 85, no. 3, 30 January 2020 (2020-01-30), pages 727 - 735, XP093034520, DOI: 10.1111/1750-3841.14842
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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