Title:
METHOD FOR REDUCING NONSPECIFIC AMPLIFICATION IN NUCLEIC ACID AMPLIFICATION REACTION
Document Type and Number:
WIPO Patent Application WO/2023/286535
Kind Code:
A1
Abstract:
A method for reducing nonspecific amplification in a nucleic acid amplification reaction according to one aspect of the present invention comprises a step for obtaining a nucleic acid extraction solution by mixing a sample containing a nucleic acid with a dodecyl alcohol sulfate ester salt and a nonionic surfactant. The nonionic surfactant is polyoxyethylene (20) sorbitan monolaurate, polyoxyethylene (10) octylphenyl ether, or n-octanoyl-N-methyl-D-glucamine. According to said method, it is possible to reduce nonspecific amplification in a nucleic acid amplification reaction when said amplification reaction is performed without performing purification of a nucleic acid extraction solution.
Inventors:
ASANO YOSHINORI (JP)
TOMITA NORIHIRO (JP)
TOMITA NORIHIRO (JP)
Application Number:
PCT/JP2022/024570
Publication Date:
January 19, 2023
Filing Date:
June 20, 2022
Export Citation:
Assignee:
EIKEN CHEMICAL (JP)
International Classes:
C12N15/10; C12Q1/6848
Foreign References:
| CN111139313A | 2020-05-12 | |||
| CN111118222A | 2020-05-08 | |||
| JP2016182112A | 2016-10-20 | |||
| JP2014097051A | 2014-05-29 |
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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