Title:
METHOD FOR REMOVING ENDOTOXIN LPS BY USING RECOMBINANT PROTEIN COMPRISING SUSHI DOMAIN OF FACTOR C PRODUCED IN PLANT
Document Type and Number:
WIPO Patent Application WO/2023/171835
Kind Code:
A1
Abstract:
The present invention is a technology related to a recombinant vector for removing LPS comprising CES3, and a LPS removal method using the vector. Particularly, a recombinant gene has been constructed to produce a recombinant protein in a plant from an N-terminal region (a cysteine-rich domain, an EGF-like domain and a domain having three sushi motifs, CES3) comprising a cysteine-rich domain, which is responsible for LPS detection and binding of a factor C protein of Carcinoscorpius rotundicauda, and a domain having Sushi 1-3 motifs, and it has been identified that a plant body transformed with the recombinant gene thereof is produced such that approximately 80% of endotoxin is removed from a protein mixture into which LPS is mixed. Therefore, a system has been constructed to fix CES:CBM3, which is a recombinant protein produced in a plant, to MCC beads, so as to remove LPS from a protein sample. The present invention is superior to a conventional LPS removal method in terms of sustainability, availability, stability and cost effectiveness.
Inventors:
HWANG IN HWAN (KR)
ISLAM KHAN MD REZAUL (KR)
ISLAM KHAN MD REZAUL (KR)
Application Number:
PCT/KR2022/003393
Publication Date:
September 14, 2023
Filing Date:
March 10, 2022
Export Citation:
Assignee:
POSTECH RES & BUSINESS DEV FOUND (KR)
International Classes:
C12N15/82; C07K14/435; C07K14/47; C12N15/62
Domestic Patent References:
| WO2001027289A2 | 2001-04-19 |
Foreign References:
| US20170283474A1 | 2017-10-05 | |||
| KR102082330B1 | 2020-02-28 | |||
| US5716834A | 1998-02-10 |
Other References:
MD REYAZUL ISLAM, JU-WON KWAK, JEON-SOO LEE, SUNG-WOOK HONG, MD REZAUL ISLAM KHAN, YONGJIK LEE, YOONTAE LEE, SEUNG-WOO LEE, INHWAN: "Cost-effective production of tag-less recombinant protein in Nicotiana benthamiana", PLANT BIOTECHNOLOGY JOURNAL, BLACKWELL PUB., GB, vol. 17, no. 6, 1 June 2019 (2019-06-01), GB , pages 1094 - 1105, XP055683185, ISSN: 1467-7644, DOI: 10.1111/pbi.13040
Attorney, Agent or Firm:
ERUUM & LEEON INTELLECTUAL PROPERTY LAW FIRM (KR)
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