Title:
METHOD FOR REMOVING N-TERMINAL TRUNCATED AND ABNORMAL VARIANTS IN RHNGF
Document Type and Number:
WIPO Patent Application WO/2019/184370
Kind Code:
A1
Abstract:
The present invention provides a method for removing N-terminal truncated and abnormal variants in rhNGF, characterized by: 1) first, washing, by means of a cleaning liquid, a rhNGF raw material loaded onto a cation exchange material to obtain a raw material from which N-terminal truncated and abnormal variants are removed, wherein the cleaning liquid is a cleaning buffer having a higher conductivity than the raw material; and 2) performing, using an elution buffer having a higher conductivity than the cleaning liquid in step 1), a cation exchange chromatography elution step on the raw material washed in step 1), collecting the eluate, and obtaining a pure rhNGF product therefrom.
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Inventors:
LIU WENCHAO (CN)
SUN HONGLIANG (CN)
ZHANG YI (CN)
WANG YUESHENG (CN)
SUN HONGLIANG (CN)
ZHANG YI (CN)
WANG YUESHENG (CN)
Application Number:
PCT/CN2018/114563
Publication Date:
October 03, 2019
Filing Date:
November 08, 2018
Export Citation:
Assignee:
XINTRUM PHARMACEUTICALS LTD (CN)
International Classes:
C07K14/48; C07K1/18; C12N11/00
Foreign References:
| CN103880943A | 2014-06-25 | |||
| CN106478801A | 2017-03-08 | |||
| US6663899B2 | 2003-12-16 |
Other References:
LI, JIA'NAN ET AL.: "Expression of Recombinant Human (3-nerve Growth Factor in Insect Cells and Purification and Biological Activity of Expressed Product", CHINESE JOURNAL OF BIOLOGICALS, vol. 26, no. 3, 31 March 2013 (2013-03-31)
Attorney, Agent or Firm:
HAIHONG JIACHENG INTELLECTUAL PROPERTY & PARTNERS (CN)
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