Title:
METHOD FOR SEQUENCING POLYNUCLEOTIDES ON BASIS OF OPTICAL SIGNAL DYNAMICS OF LUMINESCENT LABEL AND SECONDARY LUMINESCENT SIGNAL
Document Type and Number:
WIPO Patent Application WO/2021/031109
Kind Code:
A1
Abstract:
Provided is a method for sequencing polynucleotides on the basis of optical signal dynamics of a luminescent label and a secondary luminescent signal, wherein different luminescent forms and luminescent timings are used to distinguish the sequential incorporation of different nucleotides, thereby realizing the determination of a polynucleotide sequence.
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Inventors:
CHEN XI (CN)
LIAO SHA (CN)
ZHANG WENWEI (CN)
CHEN AO (CN)
ZHAO JIE (CN)
LIAO SHA (CN)
ZHANG WENWEI (CN)
CHEN AO (CN)
ZHAO JIE (CN)
Application Number:
PCT/CN2019/101530
Publication Date:
February 25, 2021
Filing Date:
August 20, 2019
Export Citation:
Assignee:
EGI TECH SHEN ZHEN CO LTD (CN)
International Classes:
C12Q1/68; C07H19/073
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Other References:
LIANJIANG TAN, YAZHI LIU, QINGLAI YANG, XIAOWEI LI, XIN-YAN WU, BING GONG, YU-MEI SHEN, ZHIFENG SHAO: "Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing", CHEMICAL COMMUNICATIONS, ROYAL SOCIETY OF CHEMISTRY, vol. 52, no. 5, 18 January 2016 (2016-01-18), pages 954 - 957, XP055610014, ISSN: 1359-7345, DOI: 10.1039/C5CC09131D
QINGLIN MENG, DAE HYUN KIM, XIAOPENG BAI, LANRONG BI, NICHOLAS J. TURRO, AND JINGYUE JU: "Design and synthesis of a photocleavable fluorescent nucleotide 3'-O-allyl-dGTP-PC-Bodipy-FL-510 as a reversible terminator for DNA sequencing by synthesis", JOURNAL OF ORGANIC CHEMISTRY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, vol. 71, no. 8, 14 April 2006 (2006-04-14), Washington, pages 3248 - 3252, XP002652986, ISSN: 0022-3263, DOI: 10.1021/JO060300K
LARRY J. KRICKA: "Chemiluminescent and Bioluminescent Techniques", CLIN. CHEM., vol. 37, no. 9, 1991, pages 1472 - 1481, XP000293036
TSUJI, A. ET AL.: "Bioluminescence and chemiluminescence: Progress and perspectives", WORLD SCIENTIFIC: [SL, 2005, pages 596
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
METZKER ET AL., NUCLEIC ACIDS RESEARCH, vol. 22, no. 20, 1994, pages 4259 - 4267
GUILLIER ET AL., CHEM. REV., vol. 100, 2000, pages 2092 - 2157
LEE ET AL., J. ORG. CHEM., vol. 64, 1999, pages 3454 - 3460
BURGESS ET AL., J. ORG. CHEM., vol. 62, 1997, pages 5165 - 5168
See also references of EP 4047098A4
QINGLIN MENG, DAE HYUN KIM, XIAOPENG BAI, LANRONG BI, NICHOLAS J. TURRO, AND JINGYUE JU: "Design and synthesis of a photocleavable fluorescent nucleotide 3'-O-allyl-dGTP-PC-Bodipy-FL-510 as a reversible terminator for DNA sequencing by synthesis", JOURNAL OF ORGANIC CHEMISTRY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, vol. 71, no. 8, 14 April 2006 (2006-04-14), Washington, pages 3248 - 3252, XP002652986, ISSN: 0022-3263, DOI: 10.1021/JO060300K
LARRY J. KRICKA: "Chemiluminescent and Bioluminescent Techniques", CLIN. CHEM., vol. 37, no. 9, 1991, pages 1472 - 1481, XP000293036
TSUJI, A. ET AL.: "Bioluminescence and chemiluminescence: Progress and perspectives", WORLD SCIENTIFIC: [SL, 2005, pages 596
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
METZKER ET AL., NUCLEIC ACIDS RESEARCH, vol. 22, no. 20, 1994, pages 4259 - 4267
GUILLIER ET AL., CHEM. REV., vol. 100, 2000, pages 2092 - 2157
LEE ET AL., J. ORG. CHEM., vol. 64, 1999, pages 3454 - 3460
BURGESS ET AL., J. ORG. CHEM., vol. 62, 1997, pages 5165 - 5168
See also references of EP 4047098A4
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (CN)
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