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Title:
METHOD FOR SEQUENCING POLYNUCLEOTIDES
Document Type and Number:
WIPO Patent Application WO/2020/093261
Kind Code:
A1
Abstract:
The present invention provides a method for sequencing polynucleotides. Sequential incorporation of different nucleotides is detected by using the same light emitting signal, thereby achieving the determination of polynucleotide sequences.

Inventors:
ZHAO JIE (CN)
LIAO SHA (CN)
ZHANG WENWEI (CN)
CHEN AO (CN)
XU CHONGJUN (CN)
FU DEFENG (CN)
Application Number:
PCT/CN2018/114281
Publication Date:
May 14, 2020
Filing Date:
November 07, 2018
Export Citation:
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Assignee:
EGI TECH SHEN ZHEN CO LTD (CN)
International Classes:
C12Q1/68
Domestic Patent References:
WO2014130388A12014-08-28
WO2000006770A12000-02-10
WO1991006678A11991-05-16
WO2002029003A22002-04-11
WO2014139596A12014-09-18
WO2004018497A22004-03-04
Foreign References:
CN103502474A2014-01-08
CN103502474A2014-01-08
CN102858995A2013-01-02
CN103602719A2014-02-26
US9453258B22016-09-27
US5302509A1994-04-12
Other References:
SARA GOODWINJOHN D. MCPHERSONW. RICHARD MCCOMBIE: "Coming of age: ten years of next-generation sequencing technologies", NATURE REVIEWS, vol. 17, 2016, pages 333 - 351c
MARTIN KIRCHERJANET KELSO: "High-throughput DNA sequencing - concepts and limitations", BIOESSAYS, vol. 32, 2010, pages 524 - 536, XP055103847, DOI: 10.1002/bies.200900181
SARA GOODWINJOHN D. MCPHERSONW. RICHARD MCCOMBIE: "Coming of age: ten years of next-generation sequencing technologies", NATURE, vol. 17, 2016, pages 333 - 351, XP055544186, DOI: 10.1038/nrg.2016.49
METZKER ET AL., NUCLEIC ACIDS RESEARCH, vol. 22, no. 20, 1994, pages 4259 - 4267
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SO APEL JRAJ AN SMARZIALI A.: "Efficient genomic DNA extraction for low target concentration bacterial cultures using SCODA DNA extraction technology", COLD SPRING HARB PROTOC, vol. 10, 2010
See also references of EP 3878968A4
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (CN)
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