Title:
METHOD FOR SIMULTANEOUS DIFFERENTIATION OF STEM CELL-DERIVED ENDOTHELIAL CELLS AND HEMANGIOBLASTS WITHOUT PURIFICATION
Document Type and Number:
WIPO Patent Application WO/2017/122887
Kind Code:
A1
Abstract:
The present invention relates to a method for the simultaneous differentiation of stem cell-derived endothelial cells and hemangioblasts without purification, the method comprising the steps of: treating stem cells with DPBS to form single cells; suspension culturing the single cells to form aggregates; classifying the aggregates into an endothelial cell differentiation group and a hemangioblast differentiation group; and differentiating the aggregates in the two classified groups. In addition, unlike a conventional method which requires a purification process for selecting desired cells in a cell differentiation process, the present invention does not require a separate purification process, thereby reducing cost and time.
Inventors:
MOON SUNG HWAN (KR)
CHUNG HYUNG MIN (KR)
GIL CHANG HYUN (KR)
CHUNG HYUNG MIN (KR)
GIL CHANG HYUN (KR)
Application Number:
PCT/KR2016/006814
Publication Date:
July 20, 2017
Filing Date:
June 27, 2016
Export Citation:
Assignee:
KONKUK UNIV GLOCAL INDUSTRY-ACADEMIC COLLABORATION FOUND (KR)
International Classes:
C12N5/071
Foreign References:
| US20110151554A1 | 2011-06-23 | |||
| US7964402B2 | 2011-06-21 |
Other References:
GIL, CHANG-HYUN ET AL.: "Well-defined Differentiation of Hesc-derived Hemangioblasts by Embryoid Body Formation without Enzymatic Treatment", BIOTECHNOLOGY LETTERS, vol. 37, no. 6, 21 February 2015 (2015-02-21), pages 1315 - 1322, XP055536813, Retrieved from the Internet
LU , SHI-JIANG ET AL.: "Generation of Functional Hemangioblasts from Human Embryonic Stem Cells", NATURE METHODS, vol. 4, no. 6, June 2007 (2007-06-01), pages 501 - 509, XP008133153
DANG, STEPHEN M. ET AL.: "Efficiency of Embryoid Body Formation and Hematopoietic Development from Embryonic Stem Cells in Different Culture Systems", BIOTECHNOLOGY AND BIOENGINEERING, vol. 78, no. 4, 20 May 2002 (2002-05-20), pages 442 - 453, XP002219286, Retrieved from the Internet
GIL, CHANG-HYUN ET AL.: "Directing Human Embryonic Stem Cells towards Functional Endothelial Cells Easily and without Purification", TISSUE ENGINEERING AND REGENERATIVE MEDICINE, vol. 13, no. 3, 9 June 2016 (2016-06-09), pages 274 - 283, XP055536818, Retrieved from the Internet
LU , SHI-JIANG ET AL.: "Generation of Functional Hemangioblasts from Human Embryonic Stem Cells", NATURE METHODS, vol. 4, no. 6, June 2007 (2007-06-01), pages 501 - 509, XP008133153
DANG, STEPHEN M. ET AL.: "Efficiency of Embryoid Body Formation and Hematopoietic Development from Embryonic Stem Cells in Different Culture Systems", BIOTECHNOLOGY AND BIOENGINEERING, vol. 78, no. 4, 20 May 2002 (2002-05-20), pages 442 - 453, XP002219286, Retrieved from the Internet
GIL, CHANG-HYUN ET AL.: "Directing Human Embryonic Stem Cells towards Functional Endothelial Cells Easily and without Purification", TISSUE ENGINEERING AND REGENERATIVE MEDICINE, vol. 13, no. 3, 9 June 2016 (2016-06-09), pages 274 - 283, XP055536818, Retrieved from the Internet
Attorney, Agent or Firm:
DARAE IP FIRM (KR)
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