Title:
METHOD FOR SIMULTANEOUSLY DETECTING EXOSOME MEMBRANE PROTEIN AND MRNA
Document Type and Number:
WIPO Patent Application WO/2020/133912
Kind Code:
A1
Abstract:
Provided in the present invention is a method for simultaneously detecting exosome membrane protein and mRNA. The present method can, in the same isolated and purified exosome sample, perform simultaneous detection using fluorescent antibody tagging of the exosome membrane protein and molecular beacon tagging of target gene mRNA included in the exosome, the molecular beacon tagging of the exosome specifically being detected using an exosome in situ capture well plate or a chip, each well of the exosome in situ capture well plate or the chip having therein a fluorescein tagging molecular beacon, the molecular beacon being a specific DNA probe detecting target gene mRNA. The present invention uses exosome in situ capture well plate and chip technology, detecting biomarker gene mRNA included in an exosome in a biological sample.
Inventors:
ZENG HENGSHAN (CN)
Application Number:
PCT/CN2019/089478
Publication Date:
July 02, 2020
Filing Date:
May 31, 2019
Export Citation:
Assignee:
HANGZHOU DIXIANG CO LTD (CN)
International Classes:
G01N33/53; C12Q1/6834; G01N33/68
Foreign References:
| CN109652504A | 2019-04-19 | |||
| CN103492590A | 2014-01-01 | |||
| CN108872438A | 2018-11-23 | |||
| CN103874770A | 2014-06-18 | |||
| CN103237901A | 2013-08-07 | |||
| CN103025890A | 2013-04-03 |
Attorney, Agent or Firm:
HANGZHOU ZHEKE PATENT AGENCY (GENERAL PARTNERSHIP) (CN)
Download PDF: