Title:
A METHOD OF SORTING CELLS
Document Type and Number:
WIPO Patent Application WO2001068226
Kind Code:
A3
Abstract:
A method of sorting cells, particularly, but not exclusively, sperm cells, is described. The method comprises the use of a surface decontamination medium to strip the cells of any extra-cellular surface contamination and to then subject to stripped cells to a charge based separation.
Inventors:
CUMMING IAN (GB)
Application Number:
PCT/GB2001/000982
Publication Date:
March 14, 2002
Filing Date:
March 08, 2001
Export Citation:
Assignee:
UNIV BRISTOL (GB)
CUMMING IAN (GB)
CUMMING IAN (GB)
International Classes:
C12N5/00; C12N5/071; A61K35/12; (IPC1-7): B01D57/02; C12Q1/24; C12N5/00; A01K67/02
Foreign References:
| US4225405A | 1980-09-30 | |||
| US4092229A | 1978-05-30 |
Other References:
MANGER M ET AL: "Effect of sperm motility on separation of bovine X- and Y-bearing spermatozoa by means of free-flow electrophoresis.", ANDROLOGIA, vol. 29, no. 1, January 1997 (1997-01-01) - February 1997 (1997-02-01), pages 9 - 15, XP001030548
ISHIJIMA S A ET AL: "Zeta potential of human X- and Y-bearing sperm", INTERNATIONAL JOURNAL OF ANDROLOGY, vol. 14, no. 5, October 1991 (1991-10-01), pages 340 - 347, XP001030547
ENGELMANN U ET AL: "Separation of human X and Y spermatozoa by free flow electrophoresis", GAMETE RESEARCH, vol. 19, no. 2, 1988, pages 151 - 160, XP001030546
BLOTTNER S ET AL: "Enrichment of bovine X and Y spermatozoa by free-flow electrophoresis.", JOURNAL OF VETERINARY MEDICINE SERIES A, vol. 41, no. 6, August 1994 (1994-08-01), pages 466 - 474, XP001030544
ISHIJIMA S A ET AL: "Zeta potential of human X- and Y-bearing sperm", INTERNATIONAL JOURNAL OF ANDROLOGY, vol. 14, no. 5, October 1991 (1991-10-01), pages 340 - 347, XP001030547
ENGELMANN U ET AL: "Separation of human X and Y spermatozoa by free flow electrophoresis", GAMETE RESEARCH, vol. 19, no. 2, 1988, pages 151 - 160, XP001030546
BLOTTNER S ET AL: "Enrichment of bovine X and Y spermatozoa by free-flow electrophoresis.", JOURNAL OF VETERINARY MEDICINE SERIES A, vol. 41, no. 6, August 1994 (1994-08-01), pages 466 - 474, XP001030544
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