METHOD FOR SYNTHESIZING CHIRAL AMINE COMPOUND

WIPO Patent Application WO/2022/166104

A1

Provided is a method for synthesizing a chiral amine compound. An amino reaction is performed on a ketone substrate by means of a transaminase under the action of an amino donor, and the chiral amine compound is obtained. A conserved amino acid sequence region of the transaminase at least comprises region 1, MAGLWCVN, and region 2, YNTFTKT. By using a transaminase with a specific conserved amino acid sequence region to synthesize the large steric hindrance chiral amine, the enzyme catalytic reaction volume is small, the synthesis route is short, the product yield is high, catalysis by means of a high-cost noble metal is not required under the synthesis condition, the three wastes are reduced, and the production cost is saved.

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HONG HAO (US)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
MA YULEI (CN)
MOU HUIYAN (CN)
WANG ZUJIAN (CN)
SUN KAIHUA (CN)
JIA RU (CN)
LIU FANG (CN)
LIU WENJING (CN)

PCT/CN2021/104842

August 11, 2022

July 06, 2021

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ASYMCHEM LAB TIANJIN CO LTD (CN)

C12P13/00; C12N9/10; C12P17/12; C12P17/14

CN112458123A	2021-03-09
CN108384767A	2018-08-10
CN110592042A	2019-12-20
CN104328093A	2015-02-04
CN110616236A	2019-12-27
CN108048419A	2018-05-18
CN112980899A	2021-06-18

DATABASE Protein 28 July 2019 (2019-07-28), ANONYMOUS: "aspartate aminotransferase family protein [Chromobacterium violaceum]", XP055956471, retrieved from Genbank Database accession no. WP_011135573
ANGELW. CHEM. INT. ED., vol. 46, 2007, pages 4367
SCIENCE, vol. 329, 2010, pages 305

KANGXIN PARTNERS, P.C. (CN)

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