Title:
METHOD FOR SYNTHESIZING CURCUMIN BY CONVERTING LIGNIN-CONTAINING BIOMASS
Document Type and Number:
WIPO Patent Application WO/2022/062087
Kind Code:
A1
Abstract:
The present invention relates to the technical field of biology, and provides a method for synthesizing curcumin by converting a lignin-containing biomass. First, a laccase gene of bacillus subtilis is knocked out, and a curcumin synthesis related gene is transferred into bacillus subtilis for genetic modification, so that a bacillus subtilis engineering bacterium having the curcumin synthesis metabolic capability is made. Also provided are a construction method for the genetically engineered bacterium, and an application of the lignin-containing biomass subjected to a catalytic treatment by a physical, chemical, or biological method, a hydrolysis product thereof, various industrial lignin, industrial lignin depolymerization products, etc. in producing curcumin. According to the method, agricultural and forest waste biomass resources such as sawdust and straw, industrial lignin, papermaking black liquor, and other cheap substances can be converted into high added-value bioactive substance curcumin, so that the high-value utilization bottleneck of agricultural and forest wastes is solved, and the production cost of curcumin is reduced, and the method has important industrial value.
Inventors:
ZHU DAOCHEN (CN)
CHEN YAN (CN)
SUN JIANZHONG (CN)
LI YIXUAN (CN)
CHEN YAN (CN)
SUN JIANZHONG (CN)
LI YIXUAN (CN)
Application Number:
PCT/CN2020/126907
Publication Date:
March 31, 2022
Filing Date:
November 06, 2020
Export Citation:
Assignee:
UNIV JIANGSU (CN)
International Classes:
C12N1/21; C12N15/52; C12N15/53; C12N15/75; C12P7/26; C12R1/125
Foreign References:
| CN103321074A | 2013-09-25 |
Other References:
LIU BIN;ZHAO YING;YU SI-LI;ZHAO GUANG-RONG: "Advances on Curcuminoids Production via Metabolic Engineering", FOOD AND FERMENTATION INDUSTRIES, vol. 42, no. 7, 25 June 2016 (2016-06-25), pages 254 - 260+268, XP055916610, DOI: 10.13995/j.cnki.11-1802/ts.201607042
HORINOUCHI SUEHARU: "Combinatorial Biosynthesis of Non-bacterial and Unnatural Flavonoids, Stilbenoids and Curcuminoids by Microorganisms", THE JOURNAL OF ANTIBIOTICS, vol. 61, no. 12, 1 December 2008 (2008-12-01), London, pages 709 - 728, XP055916611, ISSN: 0021-8820, DOI: 10.1038/ja.2008.85
DATABASE Nucleotide 7 April 2019 (2019-04-07), ANONYMOUS: "Synthetic construct clone 4CL2 4-coumarate CoA ligase gene, complete cds", XP055916615, retrieved from Genbank Database accession no. MK035998
DATABASE Nucleotide 11 May 2015 (2015-05-11), ANONYMOUS: "Synthetic construct curcuminoid synthase (cus) gene, complete cds", XP055916617, retrieved from Genbank Database accession no. KP284565
ESPARAN VIDA, KRINGS ULRICH, STRUCH MARLENE, BERGER RALF: "A Three-Enzyme-System to Degrade Curcumin to Natural Vanillin", MOLECULES, vol. 20, no. 4, 1 January 2015 (2015-01-01), pages 6640 - 6653, XP055916619, DOI: 10.3390/molecules20046640
RODRIGUES J. L., PRATHER K. L. J., KLUSKENS L. D., RODRIGUES L. R.: "Heterologous Production of Curcuminoids", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 79, no. 1, 1 March 2015 (2015-03-01), US , pages 39 - 60, XP055916621, ISSN: 1092-2172, DOI: 10.1128/MMBR.00031-14
HORINOUCHI SUEHARU: "Combinatorial Biosynthesis of Non-bacterial and Unnatural Flavonoids, Stilbenoids and Curcuminoids by Microorganisms", THE JOURNAL OF ANTIBIOTICS, vol. 61, no. 12, 1 December 2008 (2008-12-01), London, pages 709 - 728, XP055916611, ISSN: 0021-8820, DOI: 10.1038/ja.2008.85
DATABASE Nucleotide 7 April 2019 (2019-04-07), ANONYMOUS: "Synthetic construct clone 4CL2 4-coumarate CoA ligase gene, complete cds", XP055916615, retrieved from Genbank Database accession no. MK035998
DATABASE Nucleotide 11 May 2015 (2015-05-11), ANONYMOUS: "Synthetic construct curcuminoid synthase (cus) gene, complete cds", XP055916617, retrieved from Genbank Database accession no. KP284565
ESPARAN VIDA, KRINGS ULRICH, STRUCH MARLENE, BERGER RALF: "A Three-Enzyme-System to Degrade Curcumin to Natural Vanillin", MOLECULES, vol. 20, no. 4, 1 January 2015 (2015-01-01), pages 6640 - 6653, XP055916619, DOI: 10.3390/molecules20046640
RODRIGUES J. L., PRATHER K. L. J., KLUSKENS L. D., RODRIGUES L. R.: "Heterologous Production of Curcuminoids", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 79, no. 1, 1 March 2015 (2015-03-01), US , pages 39 - 60, XP055916621, ISSN: 1092-2172, DOI: 10.1128/MMBR.00031-14
Attorney, Agent or Firm:
NANJING IPZHI INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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