Title:
METHOD AND SYSTEM FOR ANALYZING METABOLITES
Document Type and Number:
WIPO Patent Application WO/2021/152936
Kind Code:
A1
Abstract:
Provided is a method for analyzing metabolites with which it is possible to analyze the metabolites of a cell without killing the cell. Provided is a method for analyzing metabolites that includes a step for culturing cells in culture broth while bringing the culture broth into contact with a liquid which is immiscible with the culture broth, a step for recovering at least part of the liquid, and a step for analyzing the cell metabolites extracted in the liquid.
Inventors:
MORINAGA HIROKO (JP)
YONEDA YASUKO (JP)
OZEKI EIICHI (JP)
YONEDA YASUKO (JP)
OZEKI EIICHI (JP)
Application Number:
PCT/JP2020/040082
Publication Date:
August 05, 2021
Filing Date:
October 26, 2020
Export Citation:
Assignee:
SHIMADZU CORP (JP)
International Classes:
C12M1/02; C12M1/26; C12M1/34; C12Q1/02; G01N27/62
Foreign References:
| JP2016096764A | 2016-05-30 |
Other References:
JANG HUI-JEONG, HA BO-KYUNG, KIM JIN-WOONG, JUNG KYUNG-HWA, AHN JIYOON, YOON SANG-HWAL, KIM SEON-WON: "Comparison of extraction phases for a two-phase culture of a recombinant E. coli producing retinoids", BIOTECHNOLOGY LETTERS, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, vol. 36, no. 3, 1 March 2014 (2014-03-01), Dordrecht, pages 497 - 505, XP055844206, ISSN: 0141-5492, DOI: 10.1007/s10529-013-1385-1
SHINDO KAZUTOSHI: "A modern purification method for volatile sesquiterpenes produced by recombinant Escherichia coli carrying terpene synthase genes", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, vol. 82, no. 6, 3 June 2018 (2018-06-03), pages 935 - 939, XP055844209, ISSN: 0916-8451, DOI: 10.1080/09168451.2017.1403882
MUNOZ, R. VILLAVERDE, S. GUIEYSSE, B. REVAH, S.: "Two-phase partitioning bioreactors for treatment of volatile organic compounds", BIOTECHNOLOGY ADVANCES., ELSEVIER PUBLISHING, BARKING., GB, vol. 25, no. 4, 24 May 2007 (2007-05-24), GB, pages 410 - 422, XP022094830, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2007.03.005
BENJAMIN L WANG, ADEL GHADERI, HANG ZHOU, JEREMY AGRESTI, DAVID A WEITZ, GERALD R FINK, GREGORY STEPHANOPOULOS: "Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption", NATURE BIOTECHNOLOGY, GALE GROUP INC., NEW YORK, vol. 32, no. 5, New York, pages 473 - 478, XP055235852, ISSN: 1087-0156, DOI: 10.1038/nbt.2857
KATO AKIHIRO, TAKATANI NOBUYUKI, IKEDA KAZUTAKA, MAEDA SHIN-ICHI, OMATA TATSUO: "Removal of the product from the culture medium strongly enhances free fatty acid production by genetically engineered Synechococcus elongatus", BIOTECHNOLOGY FOR BIOFUELS, vol. 10, no. 1, 1 December 2017 (2017-12-01), pages 141, XP055844211, DOI: 10.1186/s13068-017-0831-z
MUÑOZ RAÚL; CHAMBAUD MARTIN; BORDEL SERGIO; VILLAVERDE SANTIAGO: "A systematic selection of the non-aqueous phase in a bacterial two liquid phase bioreactor treating α-pinene", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 79, no. 1, 1 May 2008 (2008-05-01), Berlin/Heidelberg, pages 33 - 41, XP037016121, ISSN: 0175-7598, DOI: 10.1007/s00253-008-1400-y
SHINDO KAZUTOSHI: "A modern purification method for volatile sesquiterpenes produced by recombinant Escherichia coli carrying terpene synthase genes", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, vol. 82, no. 6, 3 June 2018 (2018-06-03), pages 935 - 939, XP055844209, ISSN: 0916-8451, DOI: 10.1080/09168451.2017.1403882
MUNOZ, R. VILLAVERDE, S. GUIEYSSE, B. REVAH, S.: "Two-phase partitioning bioreactors for treatment of volatile organic compounds", BIOTECHNOLOGY ADVANCES., ELSEVIER PUBLISHING, BARKING., GB, vol. 25, no. 4, 24 May 2007 (2007-05-24), GB, pages 410 - 422, XP022094830, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2007.03.005
BENJAMIN L WANG, ADEL GHADERI, HANG ZHOU, JEREMY AGRESTI, DAVID A WEITZ, GERALD R FINK, GREGORY STEPHANOPOULOS: "Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption", NATURE BIOTECHNOLOGY, GALE GROUP INC., NEW YORK, vol. 32, no. 5, New York, pages 473 - 478, XP055235852, ISSN: 1087-0156, DOI: 10.1038/nbt.2857
KATO AKIHIRO, TAKATANI NOBUYUKI, IKEDA KAZUTAKA, MAEDA SHIN-ICHI, OMATA TATSUO: "Removal of the product from the culture medium strongly enhances free fatty acid production by genetically engineered Synechococcus elongatus", BIOTECHNOLOGY FOR BIOFUELS, vol. 10, no. 1, 1 December 2017 (2017-12-01), pages 141, XP055844211, DOI: 10.1186/s13068-017-0831-z
MUÑOZ RAÚL; CHAMBAUD MARTIN; BORDEL SERGIO; VILLAVERDE SANTIAGO: "A systematic selection of the non-aqueous phase in a bacterial two liquid phase bioreactor treating α-pinene", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 79, no. 1, 1 May 2008 (2008-05-01), Berlin/Heidelberg, pages 33 - 41, XP037016121, ISSN: 0175-7598, DOI: 10.1007/s00253-008-1400-y
Attorney, Agent or Firm:
FUKAMI PATENT OFFICE, P.C. (JP)
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