METHOD OF TESTING ACTIVITY OF DOUBLE STRAND BREAK-GENERATING REAGENT

Title:

METHOD OF TESTING ACTIVITY OF DOUBLE STRAND BREAK-GENERATING REAGENT

Document Type and Number:

WIPO Patent Application WO/2020/228844

Kind Code:

A2

Abstract:

The present invention relates to a method of testing the activity of a double strand break-generating reagent. By means of the design of an extended primer and a random-sequence molecular beacon, the invention is able to achieve dual testing of the editing efficiency and the specificity of the reagent.

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Inventors:

HU JIAZHI (CN)
YIN JIANHANG (CN)
LIU MENGZHU (CN)
LIU YANG (CN)

Application Number:

PCT/CN2020/098360

Publication Date:

November 19, 2020

Filing Date:

June 28, 2020

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Assignee:

UNIV BEIJING (CN)

International Classes:

C12N15/85; C12Q1/66; C12Q1/68

Attorney, Agent or Firm:

UNITALEN ATTORNEYS AT LAW (CN)

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