Title:
METHOD FOR TRANS-DIFFERENTIATING NON-NEURONAL CELLS INTO NEURONS AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/046393
Kind Code:
A1
Abstract:
Provided is a method for trans-differentiating non-neuronal cells of a mammal into neurons. The method comprises: providing a negative regulator capable of reducing the expression of negative regulatory genes, the negative regulatory gene including RCOR1, RCOR2, RCOR3, Sin3a, Sin3b, HDAC1, HDAC2, KDM1A, PHF21A, BAF53a, G9a, USP14, HuR, BrG1, EZH2, CDYL, or HMG20B; and/or providing a positive regulator capable of improving the expression of positive regulatory genes, the positive regulatory genes including DPYSL2, BAF45b, SCF, HuB, HuC, HuD, CYP1B1, or BTRC. An effective amount of the negative regulator or the positive regulator contacting the non-neuronal cells can induce trans-differentiation of the non-neuronal animal cells into neurons.
Inventors:
ZHOU HAIBO (CN)
HU XINDE (CN)
SU JINLIN (CN)
HU XINDE (CN)
SU JINLIN (CN)
Application Number:
PCT/CN2023/115930
Publication Date:
March 07, 2024
Filing Date:
August 30, 2023
Export Citation:
Assignee:
SHANGHAI GENEMAGIC BIOSCIENCES CO LTD (CN)
International Classes:
C12N5/0793; A61P25/00; C12N5/079; C12N15/113
Domestic Patent References:
| WO2022171167A1 | 2022-08-18 | |||
| WO2021222831A2 | 2021-11-04 | |||
| WO2014071157A1 | 2014-05-08 |
Foreign References:
| CN106029880A | 2016-10-12 |
Other References:
DAS, E. ET AL.: "MicroRNA-432 Contributes to Dopamine Cocktail and Retinoic Acid Induced Differentiation of Human Neuroblastoma Cells by Targeting NESTIN and RCOR1 Genes", FEBS LETTERS, vol. 5898, 18 March 2014 (2014-03-18), pages 1706 - 1714, XP028646843, DOI: 10.1016/j.febslet.2014.03.015
QUKE XI: "Neurons Converted from Glial Cells may Treat Parkinson's Disease", WORLD SCIENCE, 10 August 2020 (2020-08-10), pages 25 - 26, XP093143666
WANG, CHUNMEI; YANG, CHUNQING; PAN, YANYOU: "The Research Progress of miRNA in Neurodegeneration Disorders", JOURNAL OF ZUNYI MEDICAL UNIVERSITY, vol. 38, no. 6, 31 December 2015 (2015-12-31), pages 555 - 559, XP009552901, ISSN: 1000-2715, DOI: 10.14169/j.cnki.zunyixuebao.2015.0132
SÁEZ JULIÁN ESTEBAN, GÓMEZ ANDREA, BARRIOS ÁLVARO, PARADA GUILLERMO, GALDAMES LEOPOLDO, GONZÁLEZ MARCELA, ANDRÉS MARÍA, PANT ADITY: "Decreased Expression of CoREST1 and CoREST2 Together with LSD1 and HDAC1/2 during Neuronal Differentiation", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 10, no. 6, 25 June 2015 (2015-06-25), US , pages e0131760, XP093143546, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0131760
QUKE XI: "Neurons Converted from Glial Cells may Treat Parkinson's Disease", WORLD SCIENCE, 10 August 2020 (2020-08-10), pages 25 - 26, XP093143666
WANG, CHUNMEI; YANG, CHUNQING; PAN, YANYOU: "The Research Progress of miRNA in Neurodegeneration Disorders", JOURNAL OF ZUNYI MEDICAL UNIVERSITY, vol. 38, no. 6, 31 December 2015 (2015-12-31), pages 555 - 559, XP009552901, ISSN: 1000-2715, DOI: 10.14169/j.cnki.zunyixuebao.2015.0132
SÁEZ JULIÁN ESTEBAN, GÓMEZ ANDREA, BARRIOS ÁLVARO, PARADA GUILLERMO, GALDAMES LEOPOLDO, GONZÁLEZ MARCELA, ANDRÉS MARÍA, PANT ADITY: "Decreased Expression of CoREST1 and CoREST2 Together with LSD1 and HDAC1/2 during Neuronal Differentiation", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 10, no. 6, 25 June 2015 (2015-06-25), US , pages e0131760, XP093143546, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0131760
Attorney, Agent or Firm:
BEIJING TANGSONG IP FIRM (CN)
Download PDF:
Previous Patent: OCCLUSION APPARATUS
Next Patent: STRENGTHENED RECEPTOR, AND IMMUNE CELL EXPRESSING STRENGTHENED RECEPTOR AND USE THEREOF
Next Patent: STRENGTHENED RECEPTOR, AND IMMUNE CELL EXPRESSING STRENGTHENED RECEPTOR AND USE THEREOF