Title:
METHOD FOR TREATING BLOOD PLASMA SAMPLE CONTAINING CLOPIDOGREL OXIDE, AND MEASUREMENT METHOD
Document Type and Number:
WIPO Patent Application WO/2023/024727
Kind Code:
A1
Abstract:
Disclosed is a method for treating a blood plasma sample containing (7aS,2'S)-2-oxo-clopidogrel, and a measurement method, which belong to the technical field of pharmaceutical analysis, and solve the problem in the prior art whereby there is not yet a method for measuring the concentration of (7aS,2'S)-2-oxo-clopidogrel in blood plasma. The method for treating a blood plasma sample containing (7aS,2'S)-2-oxo-clopidogrel comprises: taking whole blood containing (7aS,2'S)-2-oxo-clopidogrel, adding a reducing agent solution to same, and collecting blood plasma to obtain a blood plasma sample. The method for measuring (7aS,2'S)-2-oxo-clopidogrel in blood plasma involves treating, by using the treatment method, a sample to be measured, and comprises the following steps: S1, treatment of whole blood, involving: taking whole blood, adding a reducing agent solution to same, centrifuging same, and collecting blood plasma; S2, pretreatment of a blood plasma sample, involving: adding a protein precipitant and an internal standard working solution to the blood plasma, swirling and then centrifuging same, and taking the supernatant; and S3, measurement using LC/MS/MS. The method of the present invention is simple, easy to operate, has good stability, specificity and sensitivity, and provides accurate measurement results.
Inventors:
ZHANG HAI (CN)
MOU XIA (CN)
MOU XIA (CN)
Application Number:
PCT/CN2022/104622
Publication Date:
March 02, 2023
Filing Date:
July 08, 2022
Export Citation:
Assignee:
CHENGDU SHIBEIKANG BIOMEDICAL TECH CO LTD (CN)
International Classes:
G01N30/06; G01N30/02; G01N30/72
Foreign References:
| CN110361491A | 2019-10-22 | |||
| CN111208219A | 2020-05-29 | |||
| CN110567780A | 2019-12-13 | |||
| CN111812223A | 2020-10-23 | |||
| CN105636937A | 2016-06-01 | |||
| CN112136046A | 2020-12-25 | |||
| CN111665303A | 2020-09-15 | |||
| CN107773761A | 2018-03-09 | |||
| ES2387294A1 | 2012-09-19 | |||
| US20210093624A1 | 2021-04-01 |
Other References:
LI YU-HAN, SONG MIN, HANG TAI-JUN: "Development of an LC−MS/MS method for determination of 2-oxo-clopidogrel in human plasma", JOURNAL OF PHARMACEUTICAL ANALYSIS, vol. 5, no. 1, 17 July 2014 (2014-07-17), pages 12 - 17, XP093039562, ISSN: 2095-1779, DOI: 10.1016/j.jpha.2014.07.004
PARK JUNG BAE, BAE SOO HYEON, JANG SU-MIN, NOH WON JUN, HONG JANG-HEE, YOON KEE DONG, KANG HAN CHANG: "Direct measurement of active thiol metabolite levels of clopidogrel in human plasma using tris(2-carboxyethyl)phosphine as a reducing agent by LC-MS/MS ", JOURNAL OF SEPARATION SCIENCE, WILEY, DE, vol. 36, no. 14, 1 July 2013 (2013-07-01), DE , pages 2306 - 2314, XP093039564, ISSN: 1615-9306, DOI: 10.1002/jssc.201300332
PARK JUNG BAE, BAE SOO HYEON, JANG SU-MIN, NOH WON JUN, HONG JANG-HEE, YOON KEE DONG, KANG HAN CHANG: "Direct measurement of active thiol metabolite levels of clopidogrel in human plasma using tris(2-carboxyethyl)phosphine as a reducing agent by LC-MS/MS ", JOURNAL OF SEPARATION SCIENCE, WILEY, DE, vol. 36, no. 14, 1 July 2013 (2013-07-01), DE , pages 2306 - 2314, XP093039564, ISSN: 1615-9306, DOI: 10.1002/jssc.201300332
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