Title:
METHOD FOR USING IMPROVED GEL ELECTROPHORESIS TO DETECT PREβ1 HIGH DENSITY LIPOPROTEIN IN SERUM, AND APPLICATION
Document Type and Number:
WIPO Patent Application WO/2017/020830
Kind Code:
A1
Abstract:
A method for separation and quantitative analysis of pre-β1-HDL in serum by nonlinear gradient gel electrophoresis. The invention has the advantages that the preparation process of the nonlinear concentration gradient independent layering is simple and easy, wherein no special mixing device is needed; the electrophoresis is implemented for less than or equal to 3.5 hours, and the quantitative method does not require any radioactive substance. The nonlinear gradient gel electrophoresis can accurately detect the preβ1-HDL, demonstrating obvious superiority in performance.
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Inventors:
CHEN YUNQIN (CN)
ZHANG XIAOJIN (CN)
ZHANG XIAOJIN (CN)
Application Number:
PCT/CN2016/093019
Publication Date:
February 09, 2017
Filing Date:
August 03, 2016
Export Citation:
Assignee:
ZHONGSHAN HOSPITAL OF FUDAN UNIV (CN)
International Classes:
G01N27/447; C07K1/26; C09B67/10
Domestic Patent References:
| WO1992010277A1 | 1992-06-25 |
Foreign References:
| CN105137070A | 2015-12-09 | |||
| CN1699406A | 2005-11-23 | |||
| CN102181173A | 2011-09-14 | |||
| CN104297327A | 2015-01-21 | |||
| US20140243233A1 | 2014-08-28 |
Other References:
OSAMU, M. ET AL.: "Pre beta 1-HDL, a Key Element of Reverse Cholesterol Transport: its Potential as A Biomarker", CLINICAL LIPIDOLOGY, vol. 5, no. 3, 30 June 2010 (2010-06-30), pages 358, ISSN: 1758-4299
CHEN, YUNQIN ET AL.: "A New Method for Detecting Serum beta-Lipoprotein Electrophoretogram", JOURNAL OF FIRST MILITARY MEDICAL UNIVERSITY, vol. 23, no. 2, 31 December 2003 (2003-12-31), pages 153 - 155
TAKASHI, M. ET AL.: "Serum Amyloid a Protein Generates Preâl High-Density Lipoprotein from a-Migrating High-Density Lipoprotein", BIOCHEMISTRY, vol. 38, 2 December 1999 (1999-12-02), pages 16958 - 16962, XP055362757
CHEN, YUNQIN ET AL.: "A New Method for Detecting Serum beta-Lipoprotein Electrophoretogram", JOURNAL OF FIRST MILITARY MEDICAL UNIVERSITY, vol. 23, no. 2, 31 December 2003 (2003-12-31), pages 153 - 155
TAKASHI, M. ET AL.: "Serum Amyloid a Protein Generates Preâl High-Density Lipoprotein from a-Migrating High-Density Lipoprotein", BIOCHEMISTRY, vol. 38, 2 December 1999 (1999-12-02), pages 16958 - 16962, XP055362757
Attorney, Agent or Firm:
INSIGHT INTELLECTUAL PROPERTY LIMITED (CN)
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