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Title:
METHODS AND COMPOSITIONS FOR THE PRODUCTION OF ADENOVIRAL VECTORS
Document Type and Number:
WIPO Patent Application WO2004104190
Kind Code:
A3
Abstract:
The present invention addresses the need to improve the yield of adenovirus when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of infection temperatures lower than 37°C in a cell culture system results in improved yields of adenovirus. In addition, it has been demonstrated that when host cells are grow in a bioreactor, initiating adenovirus infection by diluting the host cells with fresh media and adenovirus results in improved yield of adenovirus. Methods of adenoviral production and purification using infection temperatures less than 37°C are disclosed. Methods of adenoviral production and purification wherein the host cells are grown in a bioreactor and adenovirus infection is initiated by diluting the host cells with fresh media and adenovirus are also disclosed.

Inventors:
ZHANG SHUYUAN (US)
PHAM HAI (US)
Application Number:
PCT/US2004/015009
Publication Date:
May 19, 2005
Filing Date:
May 14, 2004
Export Citation:
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Assignee:
INTROGEN THERAPEUTICS INC (US)
ZHANG SHUYUAN (US)
PHAM HAI (US)
International Classes:
C12N7/01; C12N7/02; C12N15/861; (IPC1-7): C12N7/02; C12N15/861
Domestic Patent References:
WO2001077304A12001-10-18
Foreign References:
Other References:
JARDON MARIO ET AL: "pH, pCO2, and temperature effect on R-adenovirus production.", BIOTECHNOLOGY PROGRESS, vol. 19, no. 1, February 2003 (2003-02-01), pages 202 - 208, XP002296609, ISSN: 8756-7938
NADEAU I ET AL: "Production of adenovirus vector for gene therapy", BIOTECHNOLOGY ADVANCES, ELSEVIER PUBLISHING, BARKING, GB, vol. 20, no. 7-8, January 2003 (2003-01-01), pages 475 - 489, XP004400157, ISSN: 0734-9750
GREEN ANTHONY P ET AL: "A new scalable method for the purification of recombinant adenovirus vectors", HUMAN GENE THERAPY, vol. 13, no. 16, 1 November 2002 (2002-11-01), pages 1921 - 1934, XP002297007, ISSN: 1043-0342
NADEAU I ET AL: "Low-protein medium affects the 293SF central metabolism during growth and infection with adenovirus", BIOTECHNOLOGY AND BIOENGINEERING, vol. 77, no. 1, 5 January 2002 (2002-01-05), pages 91 - 104, XP002317318, ISSN: 0006-3592
GARNIER A ET AL: "SCALE-UP OF THE ADENOVIRUS EXPRESSION SYSTEM FOR THE PRODUCTION OF RECOMBINANT PROTEIN IN HUMAN 293S CELLS", CYTOTECHNOLOGY, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, NL, vol. 15, no. 1-3, 1994, pages 145 - 155, XP001088662, ISSN: 0920-9069
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