Title:
METHODS AND COMPOSITIONS FOR PURIFYING DNA
Document Type and Number:
WIPO Patent Application WO/2012/083198
Kind Code:
A3
Abstract:
Provided are methods and compositions for extracting or purifying a polynucleotide. Further provided are methods and compositions for extracting a lipopolysaccharide from a solution containing a polynucleotide. The methods may comprise contacting a cell or cell supsension with a composition comprising a non-ionic detergent (NID). The methods may comprise contacting a solution containing a polynucleotide with a composition comprising a Hofmeister series salt (HSS), and contacting the resulting solution with a composition comprising a non-ionic detergent (NID).
Inventors:
LEZIN GEORGE (US)
BRUNELLI LUCA (US)
KUEHN MICHAEL R (US)
BRUNELLI LUCA (US)
KUEHN MICHAEL R (US)
Application Number:
PCT/US2011/065547
Publication Date:
April 10, 2014
Filing Date:
December 16, 2011
Export Citation:
Assignee:
UNIV UTAH RES FOUND (US)
LEZIN GEORGE (US)
BRUNELLI LUCA (US)
KUEHN MICHAEL R (US)
LEZIN GEORGE (US)
BRUNELLI LUCA (US)
KUEHN MICHAEL R (US)
International Classes:
C07H21/00; C12Q1/68
Foreign References:
| US20100286380A1 | 2010-11-11 |
Other References:
WARD ET AL.: "Single-step purification of shuttle vectors from yeast for high frequency back- transformation into E. coli.", NUCLEIC ACIDS RES., vol. 18, no. 17, 11 September 1990 (1990-09-11), pages 5319
Attorney, Agent or Firm:
SINGER, Christopher, P. (100 East Wisconsin Avenue Suite 330, Milwaukee WI, US)
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