METHODS FOR DECREASING OCULAR TOXICITY OF ANTIBODY DRUG

CONJUGATES

CROSS REFERENCE TO RELATED APPLICATIONS

[01] This application claims the benefit of U.S. Provisional Appl. No. 61/471 ,673, filed April 4, 201 1 which is herein incorporated by reference.

FIELD OF THE INVENTION

[02] The present invention relates to the identification that inclusion of at least one charged group on a cross linker decreases ocular toxicity associated with administration of an antibody drug conjugate.

BACKGROUND OF THE INVENTION

[03] The bifunctional modification reagent N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) has been used to link two proteins together through a disulfide bond. The reagent is reacted with the first protein to introduce an active disulfide-containing group in the modification step. A second protein, which contains a free thiol group, is then added to form a disulfide bond between the two proteins in the conjugation step. Many derivatives of SPDP and imide versions of SPDP have been described (U.S. Patent 4,563,304; J. Carlsson et al. 173 Biochem. J. 723-737 (1978); Goff D. A., Carroll, S. F. 1 BioConjugate Chem. 381- 386 (1990); L. Delprino et al. 82 J Pharm. Sci. 506-512 (1993); S. Arpicco et al., 8

BioConjugate Chem 327-337 (1997)).

[04] Conjugates of cell-binding agents with highly cytotoxic drugs have been described (U.S. Patent Nos. 5,208,020, 5,416,064; 5,475,092, 5,585,499, 6,436,931 ,

6,372,738 and 6,340,701 ; R.V.J. Chari et al., 52 Cancer Res. 127-131 (1992)). In these conjugates, the cell-binding agents are first modified with a bifunctional agent such as SPDP, SPP or SMCC to introduce an active disulfide or a maleimido moiety. Reaction with a thiol- containing cytotoxic drug provides a conjugate in which the cell-binding agent, such as a monoclonal antibody, and drug are linked via disulfide bonds or thioether bonds.

[05] Heterobifunctional cross-linkers comprising a nitropyridyldithio,

dinitropyridyldithio, N.N-dialkylcarboxamidopyridyldithio or di-(N. V-dialkylcarboxamido) pyridyldithio group and a reactive carboxylic ester group such as a N-succinimidyl ester group or a N-sulfosuccinimidyl ester group have been described (U.S. Patent No. 6,913,748). The presence of a JV-sulfosuccinimidyl group was claimed to provide higher aqueous solubility to these cross-linkers. However, once the cell-binding agent has been reacted with these cross-linkers, the N-sulfosuccinimidyl group is displaced and the solubility advantage is lost, both for the modified cell-binding agent and its drug conjugate. Since cytotoxic drugs used in cell-binding agent-drug conjugates are often only sparingly soluble in aqueous solutions, it is often difficult to link a sufficient number of drug molecules to the cell-binding agent and still maintain aqueous solubility. In addition, reactions have to be conducted in dilute solutions, which are cumbersome to scale up because of the need to use large volumes of solution.

SUMMARY OF THE INVENTION

[06] The present invention provides a method of administering an antibody drug conjugate (ADC) of the following formula CB - L - DM4 or DM4 - L - CB to a mammal, wherein CB is a cell binding agent, L is a linker containing at least one charged group, and DM4 is N(2')-deacetyl-N2'-(4-mercapto-4-methyl-l-oxopentyl)-maytans ine, said method comprising administering said ADC at a dose or frequency equivalent to a dose or frequency of an ADC, which has the same CB and DM4, but the linker does not contain at least one charged group, that induces ocular toxicity when administered to a subject of the same mammalian species. In some embodiments the mammals are humans or rabbits.

[07] The invention also provides a method of inhibiting tumor growth in a subject comprising administering an ADC of the following formula CB - L - DM4 or DM4 - L - CB to said subject, wherein CB is a cell binding agent, L is a linker containing at least one charged group, and DM4 is N(2')-deacetyl-N2'-(4-mercapto-4-methyl-l-oxopentyl)- maytansine, said method comprising administering said ADC at a dose or frequency equivalent to a dose or frequency of an ADC, which has the same CB and DM4, but the linker does not contain at least one charged group, that induces ocular toxicity when administered to a subject of the same mammalian species. In some embodiments the mammals are humans or rabbits.

[08] The invention also provides a method of reducing ADC-induced side effects or toxicity arising from the use of an ADC, said method comprising administering to a subject an ADC at a dosage of 4.3 mg/kg or greater wherein said ADC comprises the formula CB - L - DM4 or DM4 - L - CB, wherein CB is a cell binding agent, L is a linker containing at least one charged group, and DM4 is N(2')-deacetyl-N2'-(4-mercapto-4-methyl- l-oxopentyi)-maytansine. In one embodiment, the dose of ADC administered is at least about 4 mg/kg. In another embodiment, the dose is between about 4 mg/kg and about 16 mg/kg. In another embodiment, the dose is between about 4 mg/kg and about 8 mg/kg. In another embodiment, the dose is between about 5 mg/kg and 6 mg/kg. In another embodiment, the dose is between about 6 mg/kg and about 8 mg/kg. In a further embodiment, the dose is between about 6 mg/kg and about 7 mg/kg. In another embodiment, the dose is between about 7 mg/kg and about 8 mg/kg. In yet another embodiment, the dose is between about 4 mg/kg and 6 mg/kg. In a further embodiment, the dose is between about 4 mg/kg and 5 mg/kg.

[09] The invention also provides a method of reducing ADC-induced side effects or toxicity arising from the use of an ADC, said method comprising administering to a subject an ADC at a frequency of at least once every 4 weeks wherein said ADC comprises the formula CB - L - DM4 or DM4 - L - CB, wherein CB is a cell binding agent, L is a linker containing at least one charged group, and DM4 is N(2')-deacetyl-N2'-(4-mercapto-4- methyl-l-oxopentyl)-maytansine. In some embodiments, the ADC is administered at a frequency of once every two weeks, once every three weeks, or once every four weeks. In one embodiment, the ADC is administered at a frequency of at least once every three weeks.

[10] In certain embodiments, administration of the ADCs of the invention comprising a charged group has a reduction in toxicity of greater than 50% compared with the equivalent dose or equivalent frequency an ADC having the same CB and DM4, but the linker does not contain at least one charged group, when administered to a subject of the same mammalian species. In one embodiment, the dose of ADC administered is at least about 4 mg/kg. In another embodiment, the dose is between about 4 mg/kg and about 16 mg/kg. In another embodiment, the dose is between about 4 mg/kg and about 8 mg/kg. In another embodiment, the dose is between about 5 mg/kg and 6 mg/kg. In another embodiment, the dose is between about 6 mg/kg and about 8 mg/kg. In a further embodiment, the dose is between about 6 mg/kg and about 7 mg/kg. In another embodiment, the dose is between about 7 mg/kg and about 8 mg/kg. In yet another embodiment, the dose is between about 4 mg/kg and 6 mg/kg. In a further embodiment, the dose is between about 4 mg/kg and 5 mg/kg.

[11] In one embodiment, the ADCs of the invention comprise a linker having a charged group selected from the group consisting of: sulfonate, phosphate, carboxyl and quaternary amine. In another embodiment, the charged group is sulfonate. In another embodiment, the linker is selected from the group consisting ofN-succinimidyl 4-(2- pyridyldithio)-2-sulfopentanoate (sulfo-SPP); N-succinimidyl 4-(2-pyridyldithio)-2- sulfobutanoate (sulfo-SPDB); and N-sulfosuccinimidyl 4-(maleimidomethyl) cyclohexanecarboxylate (sulfoSMCC).

[12] In one embodiment, the cell binding agent is an antibody, or antigen binding fragment thereof. In another embodiment, the antibody binds an antigen selected from the group consisting of: Folate receptor 1, CanAg, EpCam, CD 19, Mesothelin, CD 138, CA6 glycotope on mucl , CD33, integrin alpha 5/beta 6, CD20, PSCA1, STEAP1, TMEF2, NGEP, and PSGR. In another embodiment, the antibody binds an antigen selected from the group consisting of: CanAg, EpCam, CD 19, Mesothelin, CD 138, CA6 glycotope on mucl, CD33, integrin alpha 5/beta 6, CD20, PSCA1 , STEAP1, TMEF2, NGEP, and PSGR. In a further embodiment, the antibody is selected from the group consisting of: huC242, huB4, MF-T, DS6, and My 9-6.

[13] In one embodiment, the invention provides methods wherein the ADC comprises a cell binding agent which is an antibody or antigen binding fragment that binds Folate receptor 1 and wherein said ADC is administered at a dose or frequency equivalent to a dose or frequency of an ADC, which has the same cell binding agent and DM4, but the linker does not contain at least one charged group, that induces ocular toxicity when administered to a subject of the same mammalian species. In one embodiment, the antibody is huMovl9 (M9346A).

[14] The invention also provides methods of decreasing ocular toxicity of ADCs using ADCs comprising the linker sulfo-SPDB.

[15] The invention also provides methods of decreasing ocular toxicity of ADCs using ADCs comprising the huDS6 antibody, a linker comprising at least one charged group, and DM4. The invention also provides methods of decreasing ocular toxicity of ADCs using ADCs comprising the huB4 antibody, a linker comprising at least one charged group, and DM4. In one embodiment, the linker is sulfo-SPDB.

[16] The invention also provides methods of decreasing ocular toxicity of ADCs using ADCs comprising the huMovl9 (M9346A) antibody, a linker comprising at least one charged group (e.g., sulfo-SPDB), and DM4 by administering the ADC at a dose or frequency that induces ocular toxicity when administered to a subject of the same mammalian species, as to an equivalent dose or frequency of an ADC having the huMovl 9 (M9346A) antibody and DM4, but the linker does not contain at least one charged group. In one embodiment, the linker is sulfo-SPDB.

BRIEF DESCRIPTION OF THE DRAWINGS

[17] Figure 1 shows the synthesis of sulfonic acid-containing cross-linking reagents that contain a nitropyridyldisulfide group and a reactive carboxylic acid ester.

Hydroxyalkanoate esters are first converted into dibromoalkanoate esters as shown, followed by conversion of the a-bromo substituent into a sulfonic acid.

[18] Figure 2 shows the synthesis of sulfonic acid-containing cross-linking reagents that contain a pyridyldisulfide group and a reactive carboxylic acid ester.

[19] Figures 3, 4 and 5 show various routes for the synthesis of charged cross- linking agents bearing a reactive carboxylic acid ester and maleimido substituent, enabling linkage via thioether bonds.

[20] Figures 6 and 7 show the synthesis of phosphate-containing cross-linking reagents that contain a pyridyldisulfide group and a reactive carboxylic acid ester.

[21] Figure 8 shows the synthesis of phosphate-containing cross-linking reagents that contain a nitropyridyldisulfide group and a reactive carboxylic acid ester

[22] Figures 9 and 10 show different routes for the synthesis of phosphate- containing charged cross-linking agents bearing a reactive carboxylic acid ester and a maleimido substituent, enabling linkage via thioether bonds.

[23] Figure 1 1 shows the synthesis of sulfonic acid-containing cross-linking reagents, where the sulfonate substituent is attached to a branched alkyl group. These reagents also bear a pyridyldisulfide group and a reactive carboxylic acid ester.

[24] Figure 12 shows the synthesis of sulfonic acid-containing cross-linking reagents, where the sulfonate substituent is attached to a branched alkyl group. These reagents also bear a reactive carboxylic acid ester and a maleimido group that allows for linkage via thioether bonds.

[25] Figure 13 shows the synthesis of quartenary amine-containing cross-linking reagents that contain a pyridyldisulfide group and a reactive carboxylic acid ester.

[26] Figure 14 shows the synthesis of quartenary amine cross-linking agents bearing a reactive carboxylic acid ester and maleimido substituent, enabling linkage via thioether bonds. [27] Figure 15 shows the synthesis of sulfonic acid-containing cross-linking reagents that contain a pyridyldisulfide group and a reactive carboxylic acid ester. In these compounds, the sulfonate substituent is on the carbon atom on the position β to the carboxyl ester.

[28] Figure 16 shows the synthesis of phosphate-containing cross-linking reagents that contain a pyridyldisulfide group and a reactive carboxylic acid ester. In these compounds, the phosphate substituent is on the β-position relative to the carboxyl ester.

[29] Figures 17, 18 and 19 show the synthesis of various sulfonic acid-containing cross-linking reagents that contain a polyethyleneglycol (PEG) chain, along with a nitropyridyldisulfide group and a reactive carboxylic acid ester.

[30] Figures 20 and 21 show the synthesis of various sulfonic acid-containing cross-linking reagents that contain a polyethyleneglycol (PEG) chain, along with a maleimido group and a reactive carboxylic acid ester.

[31] Figure 22 shows the synthesis of phosphate-containing cross-linking reagents, where the phosphate substituent is attached to an aromatic group. These reagents also bear a reactive carboxylic acid ester and a nitropyridyldithio group that allows for linkage via disulfide bonds.

[32] Figure 23 shows the synthesis of phosphate-containing cross-linking reagents, where the phosphate substituent is attached to a branched alkyl group. These reagents also bear a reactive carboxylic acid ester and a nitropyridyldithio group that allows for linkage via disulfide bonds.

[33] Figures 24 - 31 show the synthesis of sulfonate-containing cross-linking reagents that also incorporate a hydrazide moiety allowing for linkage via acid-labile bonds.

[34] Figures 32 - 36 show the synthesis of phosphate-containing cross-linking reagents that also incorporate a hydrazide moiety allowing for linkage via acid-labile bonds.

[35] Figures 37 - 38 show the synthesis of quartenary amine-containing cross- linking reagents that also incorporate a hydrazide moiety allowing for linkage via acid-labile bonds.

[36] Figures 39 - 42 show the synthesis of charged cross-linking reagents that also incorporate a polyethyleneglycol (PEG) moiety.

[37] Figures 43-44 show the synthesis of phosphate-containing cross-linking reagents, where the phosphate substituent is attached to an aromatic residue or to an alkyl group. These reagents also bear a reactive carboxylic acid ester and a nitropyridyldithio group that allows for linkage via disulfide bonds.

[38] Figures 45-49 show the synthesis of charged cross-linking agents bearing reactive carboxylic acid ester and a haloacetyl substituent, enabling linkage via thioether bonds.

[39] Figure 50 shows the synthesis of a procharged linker that would generate a negatively charged carboxylate metabolite.

[40] Figure 51 shows a conjugate of linker 158 to a drug and a monoclonal antibody and how the conjugate would be processed in the lysosome of a target cell to give a metabolite containing the drug bearing a negatively charged carboxylate.

[41] Figure 52 shows the synthesis of a procharged linker that would generate a positively charged amine-containing metabolite.

[42] Figure 53 shows a conjugate of a procharged linker to a drug and a monoclonal antibody and how the conjugate would be processed in the lysosome of a target cell to give a metabolite of the drug bearing a positively charged amine.

[43] Figure 54 shows the synthesis of a procharged linker that would generate a charged carboxylate metabolite.

[44] Figure 55 shows a conjugate of linker 172 to a drug and a moloclonal antibody and how the conjugate would be processed in the lysosome of a target cell to give a metabolite containing the drug bearing a carboxylic acid and a lysine residue.

[45] Figure 56 shows the use of charged linker in modifying a cell-binding agent and producing a cell-binding agent-drug conjugate bearing a charged linker.

[46] Figure 57 shows the in vitro potency of cell-binding agent-drug conjugates in which a charged crosslinker is incorporated.

[47] Figure 58 shows the in vitro potency and target selectivity of cell-binding agent-drug conjugates bearing a charged crosslinker.

[48] Figure 59 shows the mass spectrum of cell-binding agent-drug conjugates bearing a charged crosslinker.

[49] Figure 60 shows the cytotoxicity of Anti-CanAg (huC242) -sulfonate linker- maytansinoid conjugates with increasing maytansinoids load (E:A) toward COLO205 cells. [50] Figure 61 shows the cytotoxicity of Anti-CanAg (huC242) -sulfonate linker- maytansinoid conjugates with increasing maytansinoids load (E:A) toward multi-drug resistant COLO205-MDR cells.

[51] Figure 62 compares cytotoxicity of Anti-CanAg (huC242) - maytansinoid conjugates with or without sulfonate group in the linker toward multi-drug resistant

COLO205-MDR cells.

[52] Figure 63 compares the cytotoxicity of Anti-EpCAM (B38.1) - maytansinoid conjugates with or without sulfonate group in linker toward multi-drug resistant COLO205- MDR cells.

[53] Figure 64 compares the cytotoxicity of Anti-EpCAM (B38.1)- maytansinoid conjugates with or without sulfonate group in linker toward multi-drug resistant HCT15 cells.

[54] Figure 65 compares the cytotoxicity of Anti-EpCAM (B38.1) - maytansinoid conjugates with or without sulfonate group in linker toward multi-drug resistant COLO205- MDR cells.

[55] Figure 66 shows the in vivo anti-tumor activity of anti-EpCAM antibody- maytansinoid conjugates on COLO205 mdr xenografts (individual tumors).

[56] Figure 67 shows the in vivo anti-tumor activity of anti-EpCAM antibody- maytansinoid conjugates on COLO205 xenografts (individual tumors).

[57] Figures 68 - 70 show the methods of synthesis of sulfonic acid-containing cross-linking reagents. These reagents bear a reactive carboxylic acid ester and a maleimido group that allows for linkage via thioether bonds.

[58] Figure 71 shows the methods of synthesis of quartenary amine -containing cross-linking reagents. These reagents also bear a reactive carboxylic acid ester and a pyridyldithio group that allows for linkage via disulfide bonds.

[59] In Figures 1-71 , wherein applicable, n reperesents 0 or an integer from 1 to 10, and m represents 0 or an integer from 1 to 2000.

[60] Figure 72 shows the pharmacokinetic parameters and plasma CanAg levels of patients with ocular toxicity.

[61] Figure 73 shows the relationship between reported ocular toxicity, plasma

CanAg levels, and IMGN242 exposure.

[62] Figure 74 shows pharmacokinetic profiles for SAR3419 at both a 160 mg/m ² and 208 mg/m dose. [63] Figure 75 shows occurrence of ocular toxicity of patients receiving SAR3419 at 160 mg/m ² , 208 mg/m ² , or 270 mg/m ² doses.

DETAILED DESCRIPTION OF THE INVENTION

[64] The conjugates disclosed herein use charged or pro-charged cross-linkers.

Examples of some suitable cross-linkers and their synthesis are shown in Figures 1 to 10. Preferably, the charged or pro-charged cross-linkers are those containing sulfonate, phosphate, carboxyl or quaternary amine substituents that significantly increase the solubility of the modified cell-binding agent and the cell-binding agent-drug conjugates, especially for monoclonal antibody-drug conjugates with 2 to 20 drugs/antibody linked. Conjugates prepared from linkers containing a pro-charged moiety would produce one or more charged moieties after the conjugate is metabolized in a cell.

[65] As disclosed herein, inclusion of these charged or pro-charged cross-linkers in antibody drug conjugates (ADCs) decreases the ocular toxicity associated with administration of the conjugates. The decrease in toxicity is important because it allows for higher exposure to the ADCs, by either higher administration dose (e.g., higher area under the curve doses), higher frequency of administration, or both.

Cross-linkers

[66] The synthetic routes to produce charged crosslinkers of the present invention are shown in Figures 1-49. Synthetic routes to produce linkers with pro-charged moieties are shown in figures 50, 52, and 54. Figures 51 , 53 and 55 show a conjugate of each of the respective pro-charged linkers with a drug and a monoclonal antibody and how these conjugates would be metabolized in a target cell to give charged metabolites. The crosslinkers possess three elements: a) a substituent that is either charged or will become charged when conjugates employing these linkers are metabolized in cells. The charge will be either anionic, such as but not limited to, carboxylate, sulfonate or phosphate, or cationic, such as but not limited to, a tertiary, quaternary, or primary amine or a nitrogen-containing heterocycle, b) a group, such as a N-hydroxysuccimimide ester, maleimido group, haloacetyl group, and hydrazide, capable of reaction with a cell-binding agent, and c) a group, such as but not limited to, a disulfide, maleimide, haloacetyl, and hydrazide, capable of reaction with a drug. The charged or pro-charged substituent can be introduced by methods described herein. For example, a sulfonate charge can be introduced by first treating a commercially available haloester compound with thioacetate to produce a thioacetyl compound, followed by oxidation of the thioacetyl group, using hydrogen peroxide, to a sulfonate group.

Phosphate containing crosslinkers can be synthesized by methods described herein. First the desired reactive group, such as but not limited to, thiol, maleimide, haloacetyl and hydrazide, is introduced by the reactions shown in Figures 6-10, followed by hydrolysis of the phosphate ester to give the charged crosslinker bearing a phosphate. A positively charged quaternary amine substituent can be introduced in the crosslinker by reaction of an amine with an α,β- unsaturated ketone (see, for example, Figures 13 and 37). Alternatively a charged amine substituent can be introduced by displacement of a halogen with the amine or nitrogen containing heterocycle of choice.

[67] Preferably, the cross-linkers are compounds of the formula (I) below:

(I)

wherein Y' represents a functional group that enables reaction with a cell- binding agent;

Q represents a functional group that enables linkage of a drug via a disulfide, thioether , thioester, peptide, hydrazone, ester, ether, carbamate or amide bond;

Ri, R ₂ , R ₃ , R ₄ , R ₅ , R ₆ , R ₇ , R ₈ , R ₉ , and Rio are the same or different and are H, linear alkyl having from 1 -6 carbon atoms, branched or cyclic alkyl having from 3 to 6 carbon atoms, linear, branched or cyclic alkenyl or alkynyl having from 2 to 6 carbon atoms, anions, such as but not limited to, S0 ₃ ^" , X-S0 ₃ ^" , OP0 ₃ ^2" , X-OP0 ₃ ^2" , P0 ₃ ^2" , X-P0 ₃ ^2" , C0 ₂ -, cations, such as but not limited to, a nitrogen containing heterocycle, N ⁺ RnRj ₂ R ₁₃ or X- N ⁺ RiiR]2Ri ₃ or a phenyl, wherein:

Rn, Ri2 and Rj ₃ are the same or different and are H, linear alkyl having from 1 to 6 carbon atoms, or branched or cyclic alkyl having from 3 to 6 carbon atoms and X represents phenyl or a linear alkyl having from 1 to 6 carbon atoms, or a branched or cyclic alkyl having from 3 to 6 carbon atoms;

1, m and n are 0 or an integer from 1 to 4; A is a phenyl or substituted phenyl, wherein the substituent is a linear alkyl having from 1 to 6 carbon atoms, or a branched or cyclic alkyl having from 3 to 6 carbon atoms, or a charged substituent selected from anions, such as but not limited to, S0 ₃ ^" . X-S0 ₃ ^" , OPO3 ^2" , X-OP0 ₃ ² \ P0 ₃ ^2" , X-PO3 ^2" , CO2-, and cations, such as but not limited to, a nitrogen containing heterocycle, N ⁺ RnRi ₂ Ri ₃ or X-N ⁺ RnRi2Ri ₃ , wherein X has the same definition as above, and wherein g is 0 or 1 ;

Z is an optional polyethyleneoxy unit of formula (OCH2CH ₂ )p, wherein p is 0 or an integer from 2 to about 1000, or F 1-E1-P-E2-F2 unit in which El and E2 are the same or different and are C=0, O, or NR14, wherein Rj ₄ is H, a linear alkyl having from 1 -6 carbon atoms, a branched or cyclic alkyl having from 3 to 6 carbon atoms, a linear, branched or cyclic alkenyl or alkynyl having from 2 to 6 carbon atoms; P is a peptide unit between 2 and 20 amino acids in length, wherein El or E2 can be linked to the peptide through the terminal nitrogen, terminal carbon or through a side chain of one of the amino acids of the peptide; and Fl and F2 are the same or different and are an optional polyethyleneoxy unit of formula (OCH ₂ CH2) _p , wherein p is 0 or an integer from 2 to about 1000, provided that when Z is not F1-E1 -P-E2-F2, at least one of R _{1 }} R2, R3, R , R ₅ , R^, R7, R ₈ , R9, and R ₁₀ is a charged substituent or when g is 1 , at least one of A, Ri, R2, R3, R4, R ₅ , ¾, R ₇ , R ₈ , R9, and Rio is a charged substituent.

[68] Examples of the functional group, Y', that enables reaction with a cell-binding agent include amine reacting agents such as but not limited to N-hydroxysuccinmide esters, p-nitrophenyl esters, dinitrophenyl esters, pentafluorophenyl esters; thiol reactive agents such as but not limited to pyridyldisulfides, nitropyridyldisulfides, maleimides, haloacetates and carboxylic acid chlorides.

[69] Examples of the functional group, Q, which enables linkage of a cytotoxic drug, include groups that enable linkage via a disulfide, thioether, thioester, peptide, hydrazone, ester, carbamate, or amide bond. Such functional groups include, but are not limited to, thiol, disulfide, amino, carboxy, aldehydes, maleimido, haloacetyl, hydrazines, and hydroxy.

[70] Examples of linear alkyls include methyl, ethyl, propyl, butyl, pentyl and hexyl. Examples of branched or cyclic alkyls having 3 to 6 carbon atoms include isopropyl, sec-butyl, isobutyl, tert-butyl, pentyl, hexyl, cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl. [71] Examples of linear alkenyls having 2 to 6 carbon atoms include_ethenyl, propenyl, butenyl, pentenyl, hexenyl. Examples of branched or cyclic alkenyls having 2 to 6 carbon atoms include isobutenyl, isopentenyl, 2-methyl-l -pentenyl, 2-methyl-2-pentenyl.

[72] Examples of linear alkynyls having 2 to 6 carbon atoms include ethynyl, propynyl, butynyl, pentynyl, hexynyl. Examples of branched or cyclic alkynyls having up to 6 carbon atoms include 3 -methyl- 1 -butynyl, 3 -methyl- 1 -penynyl, 4-methyl-2-hexynyl.

[73] In preferred embodiments, one of R _{l s} R ₂ , R ₃ , R ₄ , R ₉ , Rio is a charged substituent selected from sulfonate, phosphate or trialkylammonium, and the rest are H, 1, g and m are each 0, n = 1 , Q and Y' are each independently, a disulfide substituent, a maleimido, a haloacetyl group, or a N-hydroxy succinimide ester. In another more preferred embodiment, one of R _{1 }} R ₂ , R ₃ , R ₄ , R ₉ , Rio is a sulfonate, and the rest are H, 1, g and m are each 0, n = 1 , Q is a disulfide, maleimido or haloacetyl moiety, and Y' is a maleimido moiety or a N-hydroxy succinimide ester. In a farther more preferred embodiment, one of R _{l s} R ₂ , R ₃ , R ₄ , R ₉ , Rio is a sulfonate, and the rest are H, 1, g and m are each 0, n = 1 , Q is a pyridyldithio or nitropyridyldithio group, maleimido or haloacetyl moiety, and Y' is a N-hydroxy succinimide ester.

[74] The synthesis of 2-dithionitropyridyl and 2-dithio-dinitropyridyl containing cross-linkers of formulae (I) is shown, for example, in Figures 1 , 2 and the synthesis of the corresponding 4-dithionitropyridyl and 4-dithio-dinitropyridyl containing cross-linkers of the formula (I) is shown, for example, in Figure 6. The synthesis of maleimido-containing charged cross linkers of the formula (I) with a sulfonate group is shown, for example, in Figures 3, 4 and 5. The synthesis of maleimido-containing charged cross linkers of the formula (I) with a phosphate group is shown, for example, in Figures 9 and 10. The synthesis of quaternary amine-containing charged crosslinkers of formula (I) is shown, for example, in Figures 13 and 14. The synthesis of polyethylene glycol-containing charged cross linkers of formula (I) are shown, for example, in Figures 17 -21. The synthesis of charged cross linkers of formula (I) bearing a hydrazide moiety enabling linkage via acid-labile bonds is shown, for example, in Figures 24-36.

Cell-binding agent drug _^

[75] Using the charged or pro-charged crosslinkers a high number (>6) of drug molecules can be introduced. In non limiting examples, Figure 57 exemplifies that cell- binding agent-drug conjugates prepared using a charged crosslinker of the present invention display high potency. In addition, the potency is target selective (see, for example, Figure 58), since, even after linkage of a high number of drug molecules, the conjugate is highly potent towards target cells, but much less potent towards non-target cells. As exemplified in Figure 59, mass spectral analysis demonstrates that the drugs are linked covalently to the cell- binding agent via the charged crosslinker.

[76] The conjugates of the present invention can be represented by the following formula, CB-(-L ^c -D) _q , wherein CB is a cell-binding agent, L ^c is a charged or pro-charged linker, D is a drug molecule, and q is an integer from 1 to 20. In certain embodiments, the cell binding agent is an antibody. In embodiments where the the cell binding agent is an antibody, the terms "antibody drug conjugate" and "drug-charged linker cell-binding agent conjugate" are used interchangeably.

[77] Preferably, the conjugates have the following formula (II):

(Π)

wherein CB represents a cell-binding agent,

D represents a drug linked to the cell-binding agent by a disulfide, thioether, thioester, peptide, hydrazone, ester, carbamate or amide bond;

Ri, R ₂ , R ₃ , Rt, R ₅ , R6, R7, R ₈ , R , and Rio are the same or different and are H, linear alkyi having from 1 -6 carbon atoms, branched or cyclic alkyl having from 3 to 6 carbon atoms, linear, branched or cyclic alkenyl or alkynyl having from 2 to 6 carbon atoms, anions, such as but not limited to, S0 ₃ \ X-S0 ₃ ^" . OPO3 ^2" , X-OP0 ₃ ^2" , P0 ₃ ^2" , X-P0 ₃ ^2' , C0 ₂ ^" , cations, such as but not limited to, a nitrogen containing heterocycle, N ⁺ RnRi2Ri3 or X- N ⁺ RnRi ₂ Ri3, or a phenyl, wherein:

R11 , Ri ₂ and R13 are same or different and are H, linear alkyl having from 1 to 6 carbon atoms, branched or cyclic alkyl having from 3 to 6 carbon atoms and X represents phenyl or a linear alkyl having from 1 to 6 carbon atoms, or a branched or cyclic alkyl having from 3 to 6 carbon atoms;

1, m and n are 0 or an integer from 1 to 4;

A is a phenyl or substituted phenyl, wherein the substituent is a linear alkyl having from 1 to 6 carbon atoms, or a branched or cyclic alkyl having from 3 to 6 carbon atoms, or a charged substituent selected from anions, such as but not limited to, S0 ₃ \ X-S0 ₃ ^* . OP0 ₃ ^2" , X-OP0 ₃ ^2" , P0 ₃ ^2" , X-PO3 ^2" , C0 ₂ ^" , cations, such as but not limited to, a nitrogen containing heterocycle, N ⁺ RnRi2Ri3 or X-N ⁺ RnRi ₂ Ri ₃ , wherein X has the same definition as above, and wherein g is 0 or 1 ;

Z is an optional polyethyleneoxy unit of formula (OCH ₂ CH2) _p , wherein p is 0 or an integer from 2 to about 1000, or F1-E1-P-E2-F2 unit in which El and E2 are the same or different and are C=0, O, or NR14, wherein Ri ₄ is H, a linear alkyl having from 1-6 carbon atoms, a branched or cyclic alkyl having from 3 to 6 carbon atoms, a linear, branched or cyclic alkenyl or alkynyl having from 2 to 6 carbon atoms; P is a peptide unit between 2 and 20 amino acids in length, wherein El or E2 can be linked to the peptide through the terminal nitrogen, terminal carbon or through a side chain of one of the amino acids of the peptide; and Fl and F2 are the same or different and are an optional polyethyleneoxy unit of formula (OCH ₂ CH ₂ ) _p , wherein p is 0 or an integer from 2 to about 1000, provided that when Z is not F1-E1-P-E2-I 2, at least one of R R ₂ , R ₃ , R4, R ₅ , R7, Rs, R9, and Rio is a charged substituent or when g is 1, at least one of A, Ri, R ₂ , R ₃ , R ₄ , R ₅ , R^, R7, R ₈ , R9, and Rio is a charged substituent;

Y represents a carbonyl, thioether, amide, disulfide, or hydrazone group; and q is an integer from 1 to 20.

[78] As described in more detail below, the drug can be any of many small molecule drugs, including, but not limited to, maytansinoids, CC-1065 analogs, morpholinos, doxorubicins, taxanes, cryptophycins, epothilones, calicheamicins, auristatins, and pyrrolobenzodiazepine dimers.

[79] In preferred embodiments, one of R _{l s} R ₂ , R3, ^, R9, Rio is a charged substituent selected from sulfonate, phosphate, carboxylate or trialkylammonium, and the rest are H, 1, g and m are each 0, n = 1 , D is a maytansinoid, a CC-1065 analog or a

pyrrolobenzodiazepine dimer. In another more preferred embodiment, one of Rj, R ₂ , R ₃ , R4, R9, Rio is a sulfonate, and the rest are H, 1, g and m are each 0, n = 1, D is a maytansinoid, CC-1065 analog or a pyrrolobenzodiazepine dimer linked via a disulfide, thioester, or thioether bond. In a further more preferred embodiment, one of Rj, R ₂ , R ₃ , R4, R9, Rio is a sulfonate, and the rest are H, 1, g and m are each 0, n ^~ 1, and Q is a maytansinoid, a CC-1065 analog, or a taxane.

[80] In a preferred embodiment, when Z is an F1-E1-P-E2-F2 unit, El and E2 are the same or different and are C=0 or NR14, wherein R[ ₄ is H, a linear alkyl having from 1-6 carbon atoms, a branched or cyclic alkyl having from 3 to 6 carbon atoms, P is a peptide unit between 2 and 8 amino acids in length, wherein El or E2 can be linked to the peptide through the terminal nitrogen, terminal carbon or through a side chain of one of the amino acids of the peptide, preferred amino acid residues are glycine (gly), alanine (ala), leucine (leu), glutamic acid (glu), or lysine (lys), which can be used in any combination or any order (e.g., gly-gly- gly or ala-leu-ala-leu, etc.); and Fl and F2 are the same or different and are an optional polyethyleneoxy unit of formula (OCH ₂ CH ₂ ) _p , wherein p is 0 or an integer from 2 to about 1000.

[81] In a more preferred embodiment, when Z is an F1-E1-P-E2-F2 unit, El and E2 are the same or different and are C=0 or NR14, wherein R ₁₄ is H or a linear alkyl having from 1-6 carbon atoms, P is a peptide unit between 2 and 5 amino acids in length, wherein El or E2 can be linked to the peptide through the terminal nitrogen, terminal carbon or through a side chain of one of the amino acids of the peptide; and Fl and F2 are the same or different and are an optional polyethyleneoxy unit of formula (OCH ₂ CH ₂ ) _p , wherein p is 0 or an integer from 2 to 24.

[82] Preferably, q, the number of drugs bound to each cell-binding agent is 1 -20, more preferably 2-18, and even more preferably 2-16, and most preferably 2-10.

[83] To synthesize the conjugate, the cell-binding agent can be modified with the crosslinkers of the present invention to introduce reactive disulfide groups, maleimido, haloacetyl or hydrazide groups. Synthesis of the cell-binding agent-drug conjugates linked via disulfide bonds is achieved by a disulfide exchange between the disulfide bond in the modified cell-binding agent and a drug containing a free thiol group. Synthesis of the cell- binding agent-drug conjugates linked via thioether is achieved by reaction of the maleimido or haloacetyl modified cell-binding agent and a drug containing a free thiol group. Synthesis of conjugates bearing an acid labile hydrazone link can be achieved by reaction of a carbonyl group with the hydrazide moiety in the linker, by methods known in the art (see, for example, P. Hamann et al, BioConjugate Chem., 13; 40-46, 2002; B. Laguzza et al, J.Med, Chem., 32; 548-555, 1959; P. Trail et al., Cancer Res., 57; 100-105, 1997).

[84] Alternatively, the drug can be modified with the crosslinkers of the present invention to give a modified drug of formula (IV) bearing a functionality capable of reacting with a cell binding agent. For example a thiol-containing drug can be reacted with the charged or pro-charged crosslinker of formula (I) bearing a maleimdo substituent at neutral pH in aqueous buffer to give a drug connected to the charged linker via a thioether link. A thiol-containg drug can undergo disulfide exchange with a charged linker bearing a pyrdiyldithio moiety to give a modified drug attached via a disulfide bond to the charged crosslinker. A drug bearing a hydroxyl group can be reacted with a charged or pro-charged crosslinker bearing a halogen, in the presence of a mild base, to give a modified drug bearing an ether link. A hydroxyl group containing drug can be condensed with a charged crosslinker of formula (I) bearing a carboxyl group, in the presence of a dehydrating agent, such as dicyclohexylcarbodimide, to give an ester link. An amino group containing drug can similarly undergo condensation with a carboxyl group on the charged or pro-charged crosslinker of formula (I) to give an amide bond.

[85] The conjugate may be purified by standard biochemical means, such as gel filtration on a Sephadex G25 or Sephacryl S300 column, adsorption chromatography, and ion exchange or by dialysis as previously described. In some cases (e.g. folic acid, melanocyte stimulating hormone, EGF etc) the cell-binding agent-drug conjugates can be purified by chromatography such as by HPLC, medium pressure column chromatography or ion exchange.

Modified cell-binding a&ents

[86] The cell-binding agent modified by reaction with crosslinkers of the present invention are preferably represented by the formula (III)

(III)

wherein the substituents are as described above for the charged or pro-charged linker and the cell-binding agent drug conjugate.

[87] In preferred embodiments, one of R _{l s} R ₂ , R ₃ , Rj, R ₉ , Rio is a charged substituent selected from sulfonate, phosphate, carboxyl or trialkylammonium, and the rest are H, 1, g and m are each 0, n = 1, Q is a disulfide substituent, a maleimido, haloacetyl group, or a N-hydroxy succinimide ester, and Y is thioether, amide, or disulfide. In another more preferred embodiment, one of R _1; R ₂ , R ₃ , R4, R ₉ , Rio is a sulfonate, and the rest are H, 1, g and m are each 0, n = 1 , Q is a disulfide, maleimido or haloacetyl moiety, and Y is thioether, amide, or disulfide. In a further more preferred embodiment, one of Ri, R ₂ , R ₃ , R4, R ₉ , Rio is a sulfonate, and the rest are H, 1, g and m are each 0, n = 1, Q is a pyridyldithio or nitropyridyldithio group, and Y is thioether, amide, or disulfide.

[88] In a preferred embodiment, when Z is an F1-E1-P-E2-F2 unit, El and E2 are the same or different and are C=0 or NR14, wherein R] ₄ is H, a linear alkyl having from 1-6 carbon atoms, a branched or cyclic alkyl having from 3 to 6 carbon atoms, P is a peptide unit between 2 and 8 amino acids in length, wherein El or E2 can be linked to the peptide through the terminal nitrogen, terminal carbon or through a side chain of one of the amino acids of the peptide, preferred amino acid residues are glycine (gly), alanine (ala), leucine (leu), glutamic acid (glu), or lysine (lys), which can be used in any combination or any order (e.g., gly-gly- gly or ala-leu-ala-leu, etc.); and Fl and F2 are the same or different and are an optional polyethyleneoxy unit of formula (OCH ₂ CH ₂ ) _p , wherein p is 0 or an integer from 2 to about 1000.

[89] In a more preferred embodiment, when Z is an F1-E1-P-E2-F2 unit, El and E2 are the same or different and are C=0 or NR14, wherein Rj ₄ is H or a linear alkyl having from 1-6 carbon atoms, P is a peptide unit between 2 and 5 amino acids in length, wherein El or E2 can be linked to the peptide through the terminal nitrogen, terminal carbon or through a side chain of one of the amino acids of the peptide; and Fl and F2 are the same or different and are an optional polyethyleneoxy unit of formula (OCH2CH ₂ ) _p , wherein p is 0 or an integer from 2 to 24.

[90] The modified cell-binding agent can be prepared by reacting the cell-binding agent with the charged crosslinkers by methods known in the art for other crosslinkers (U.S. Patent Nos. 6,340,701 Bl, 5,846,545, 5,585,499, 5,475,092, 5,414,064, 5,208,020, and 4,563,304; R.V.J. Chari et al. Cancer Research 52, 127-131 , 1992; R.V.J. Chari et al. Cancer Research 55, 4079-4084, 1995; J. Carlsson et al. 173 Biochem. J. (1978) 723-737(1978); Goff, D. A., Carroll, S. F. 1 BioConjugate Chem. 381-386 (1990); L. Delprino et al. 82 J Pharm. Sci. 506-512 (1993); S. Arpicco et al., 8 BioConjugate Chem 527-337 (1997)).

Advantageously, because the cross-linker groups are soluble in water or require only a small percentage of organic solvent to maintain solubility in aqueous solution, the reaction between the cell-binding agent and the cross-linker can be conducted in aqueous solution. The cross- linking reagent is dissolved in aqueous buffer, optionally containing a small amount

(typically <10% by volume) of a polar organic solvent that is miscible with water, for example different alcohols, such as methanol, ethanol, and propanol, dimethyl formamide, dimethyl acetamide, or dimethylsulfoxide at a high concentration, for example 1-100 mM, and then an appropriate aliquot is added to the buffered aqueous solution of the cell-binding agent. An appropriate aliquot is an amount of solution that introduces 1-10 cross-linking groups per cell-binding agent, preferably 1-5 groups, and the volume to be added should not exceed 10 %, preferably 5 %, and most preferably 0-3 % of the volume of the cell-binding agent solution. The aqueous solutions for the cell-binding agents are buffered between pH 6 and 9, preferably between 6.5 and 7.5 and can contain any non-nucleophilic buffer salts useful for these pH ranges. Typical buffers include phosphate, triethanolamine.HCl, HEPES, and MOPS buffers, which can contain additional components, such as sucrose and salts, for example, NaCl. After the addition the reaction is incubated at a temperature of from 4 °C to 40 °C, preferably at ambient temperature. The progress of the reaction can be monitored by measuring the increase in the absorption at 495 nm or another appropriate wavelength. After the reaction is complete, isolation of the modified cell-binding agent can be performed in a routine way, using for example gel filtration chromatography, or adsorptive chromatography.

[91] The extent of modification can be assessed by measuring the absorbance of the nitropyridine thione, dinitropyridine dithione, carboxamidopyridine dithione or

dicarboxamidopyridine dithione group released. In a non limiting example, Figure 56 shows the results from the modification of the cell-binding agent, the C242 antibody, with a sulfonate crosslinker of the present invention. The time course of linker/antibody (L/A) incorporation is shown, for example, along with the drugs/antibody (D/A) linked. The charged or pro-charged crosslinkers described herein have diverse functional groups that can react with any cell-binding agent that possesses a suitable substituent. For example cell- binding agents bearing an amino or hydroxyl substituent can react with crosslinkers bearing an N-hydroxysuccinimide ester, cell-binding agents bearing a thiol substituent can react with crosslinkers bearing a maleimido or haloacetyl group. Additionally, cell-binding agents bearing a carbonyl substituent can react with crosslinkers bearing a hydrazide. One skilled in the art can readily determine which crosslinker to use based on the known reactivity of the available functional group on the cell-binding agent.

[92] Crosslinkers bearing a positive charge (for example compound 214, Figure 71) can be directly reacted with a cell binding agent in aqueous buffer at a pH between 5 and 9, optionally containing an organic cosolvent (such as 1 to 20% dimethylaceatmide or ethanol) to provide a modified cell binding agent bearing a positive charge and a thiol group. The thiol group of the cell binding agent can be reacted with a cytotoxic drug bearing either a maleimido, haloacetamido or an active disulfide (example pyridyldithio, nitropyridyldithio group) to provide a conjugate. The conjugate can be purified by the methods described above.

[93] Alternatively, crosslinkers bearing a positive charge and a reactive ester

(example compound 216, Figure 71) can be directly reacted with a cell binding agent (through its lysine amino group) to introduce a positive charge and an active disulfide.

Reaction with a thiol-containing cytotoxic drug as described above can provide a conijugate bearing a positive charge.

Modified Cytotoxic Dni£s

[94] The cytotoxic drugs modified by reaction with crosslinkers of the present invention are preferably represented by the formula (IV):

wherein the substituents are as described above for the charged or pro-charg linker and the cell-binding agent drug conjugate. [95] In preferred embodiments, one of Ri, R ₂ , R ₃ , R4, R9, Rio is a charged substituent selected from sulfonate, phosphate, carboxyl or trialkylammonium, and the rest are H, 1, g and m are each 0, n™ 1 , and Y' is a disulfide substituent, a maleimido, haloacetyl group, or a N-hydroxy succinimide ester. In another more preferred embodiment, one of Ri, R ₂ , R3, R ₄ , R9, Rio is a sulfonate, and the rest are H, 1, g and m are each 0, n = 1 , and Y' is a maleimido moiety or a N-hydroxy succinimide ester. In a further more preferred

embodiment, one of R _ls R ₂ , R3, R4, R ₉ , R ₁₀ is a sulfonate, and the rest are H, 1, g and m are each 0, n = 1 , and Y' is a N-hydroxy succinimide ester.

[96] In a preferred embodiment, when Z is an F1-E1-P-E2-F2 unit, El and E2 are the same or different and are C=0 or NR14, wherein Ri ₄ is H, a linear alkyl having from 1-6 carbon atoms, a branched or cyclic alkyl having from 3 to 6 carbon atoms, P is a peptide unit between 2 and 8 amino acids in length, wherein El or E2 can be linked to the peptide through the terminal nitrogen, terminal carbon or through a side chain of one of the amino acids of the peptide, preferred amino acid residues are glycine (gly), alanine (ala), leucine (leu), glutamic acid (glu), or lysine (lys), which can be used in any combination or any order (e.g., gly-gly- gly or ala-leu-ala-leu, etc.); and Fl and F2 are the same or different and are an optional polyethyleneoxy unit of formula (OCH ₂ CH ₂ ) _p , wherein p is 0 or an integer from 2 to about 1000.

[97] In a more preferred embodiment, when Z is an F1-E1-P-E2-F2 unit, El and E2 are the same or different and are C=0 or NR14, wherein Ri ₄ is H or a linear alkyl having from 1-6 carbon atoms, P is a peptide unit between 2 and 5 amino acids in length, wherein El or E2 can be linked to the peptide through the terminal nitrogen, terminal carbon or through a side chain of one of the amino acids of the peptide; and Fl and F2 are the same or different and are an optional polyethyleneoxy unit of formula (OCH ₂ CH ₂ ) _p , wherein p is 0 or an integer from 2 to 24.

[98] The modified drugs can be prepared by reacting the drug with the crosslinkers of the present invention to give a modified drug of formula (IV) bearing a functionality capable of reacting with a cell binding agent. For example a thiol-containing drug can be reacted with the charged or pro-charged crosslinker of formula (I) bearing a maleimdo substituent at neutral pH in aqueous buffer to give a drug connected to the charged or pro- charged linker via a thioether link. A thiol-containg drug can undergo disulfide exchange with a charged or pro-charged linker bearing a pyrdiyldithio moiety to give a modified drug attached via a disulfide bond to the charged or pro-charged crosslinker. A drug bearing a hydroxyl group can be reacted with a charged crosslinker bearing a halogen, in the presence of a mild base, to give a modified drug bearing an ether link. A hydroxyl group containing drug can be condensed with a charged crosslinker of formula (I) bearing a carboxyl group, in the presence of a dehydrating agent, such as dicyclohexylcarbodimide, to give an ester link. An amino group containing drug can similarly undergo condensation with a carboxyl group on the charged or pro-charged crosslinker of formula (I) to give an amide bond. The modified drug can be purified by standard methods such as column chromatography over silica gel or alumina, crystallization, preparatory thin layer chromatography, ion exchange chromatography or HPLC.

Cell-binding Agents

[99] The cell-binding agent that comprises the conjugates and the modified cell- binding agents of the present invention may be of any kind presently known, or that become known, and includes peptides and non-peptides. The cell-binding agent may be any compound that can bind a cell, either in a specific or non-specific manner. Generally, these can be antibodies (especially monoclonal antibodies and antibody fragments), interferons, lymphokines, hormones, growth factors, vitamins, nutrient-transport molecules (such as transferrin), or any other cell-binding molecule or substance.

[100] Where the cell-binding agent is an antibody, it binds to an antigen that is a polypeptide and may be a transmembrane molecule (e.g. receptor) or a ligand such as a growth factor. Exemplary antigens include molecules such as renin; a growth hormone, including human growth hormone and bovine growth hormone; growth hormone releasing factor; parathyroid hormone; thyroid stimulating hormone; lipoproteins; alpha- 1 -antitrypsin; insulin A-chain; insulin B-chain; proinsulin; follicle stimulating hormone; calcitonin; luteinizing hormone; glucagon; clotting factors such as factor vmc, factor IX, tissue factor (TF), and von Willebrands factor; anti-clotting factors such as Protein C; atrial natriuretic factor; lung surfactant; a plasminogen activator, such as urokinase or human urine or tissue- type plasminogen activator (t-PA); bombesin; thrombin; hemopoietic growth factor; tumor necrosis factor-alpha and -beta; enkephalinase; RANTES (regulated on activation normally T-cell expressed and secreted); human macrophage inflammatory protein (MIP-1 -alpha); a serum albumin, such as human serum albumin; Muellerian-inhibiting substance; relaxin A- chain; relaxin B-chain; prorelaxin; mouse gonadotropin-associated peptide; a microbial protein, such as beta-lactamase; DNase; IgE; a cytotoxic T-lymphocyte associated antigen (CTLA), such as CTLA-4; inhibin; activin; vascular endothelial growth factor (VEGF); receptors for hormones or growth factors; protein A or D; rheumatoid factors; a neurotrophic factor such as bone-derived neurotrophic factor (BDNF), neurotrophin-3, -4, -5, or -6 (NT-3, NT4, NT-5, or NT-6), or a nerve growth factor such as NGF-β; platelet-derived growth factor (PDGF); fibroblast growth factor such as aFGF and PFGF; epidermal growth factor (EGF); transforming growth factor (TGF) such as TGF-alpha and TGF-beta, including TGF-βΙ , TGF-p2, TGF- β3, TGF-P4, or TGF- β5; insulin-like growth factor-I and -II (IGF-I and IGF- II); des(l-3)-IGF-I (brain IGF-I), insulin-like growth factor binding proteins, EpCAM, GD3, FLT3, PSMA, PSCA, MUC1 , MUC16, STEAP, CEA, TENB2, EphA receptors, EphB receptors, folate receptor, mesothelin, cripto, alpha _v beta<5, integrins, VEGF, VEGFR, tarnsferrin receptor, IRTA1, 1RTA2, IRTA3, IRTA4, IRTA5; CD proteins such as CD2, CD3, CD4, CD5, CD6, CD8, CD1 1 , CD14, CD19, CD20, CD21 , CD22, CD25, CD26, CD28, CD30, CD33, CD36, CD37, CD38, CD40, CD44, CD52, CD55, CD56, CD59, CD70, CD79, CD80, CD81 , CD103, CD105, CD134, CD137, CD138, CD152 or an antibody which binds to one or more tumor-associated antigens or cell-surface receptors disclosed in US Publication No. 20080171040 or US Publication No. 20080305044 and are incorporated in their entirety by reference; erythropoietin; osteoinductive factors; immunotoxins; a bone morphogenetic protein (BMP); an interferon, such as interferon-alpha, -beta, and -gamma; colony stimulating factors (CSFs), e.g., M-CSF, GM-CSF, and G-CSF; interleukins (ILs), e.g., IL-1 to IL-10; superoxide dismutase; T-cell receptors; surface membrane proteins; decay accelerating factor; viral antigen such as, for example, a portion of the HIV envelope; transport proteins; homing receptors; addressins; regulatory proteins; integrins, such as CD 1 1 a, CD l ib, CD 1 1 c, CD 18, an ICAM, VLA-4 and VCAM; a tumor associated antigen such as HER2, HER3 or HER4 receptor; and fragments of any of the above-listed polypeptides.

[101] Preferred antigens for antibodies encompassed by the present invention include CD proteins, such as CD3, CD4, CD8, CD19, CD20, CD34, and CD46; prostate antigens, including but not limited to PSCA2, STEAP 1 (STAMP), TMEF2, NGEP, and PSGR; CanAg; members of the ErbB receptor family, such as the EGF receptor, HER2, HER3 or HER4 receptor; cell adhesion molecules, such as EpCAM, LFA-1, Macl, pi 50.95, VLA-4, ICAM-1, VCAM, alpha4/beta7 mtegrin, alpha 5/beta6 integrin, and alpha v/beta3 integrin including either alpha or beta subunits thereof (e.g. anti-CD 1 la, anti-CD 18 or anti- CD1 lb antibodies); growth factors, such as VEGF; mesothelin; Folate receptor 1 ; tissue factor (TF); TGF-β.; alpha interferon (alpha-IFN); an interleukin, such as IL-8; IgE; blood group antigens Apo2, death receptor; flk2/flt3 receptor; obesity (OB) receptor; mpl receptor; CTLA-4; protein C etc. The most preferred targets herein are IGF-IR, CanAg, VEGF, TF, CD19, CD20, CD33, CD40, CD56, CD138, CA6, Her2/neu, TGF- β, CDl la, CD18, Apo2 and C24. In some embodiments, Folate receptor 1 is not a preferred target.

[102] Additional examples of cell-binding agents that can be used include:

-resurfaced antibodies (U.S. patent no. 5,639,641);-humanized or fully human antibodies, selected from but not limited to, huMy9-6, huB4, huC242, huN901, DS6, CD38, IGF-IR, CNTO 95, B-B4, anti-FOLRl antibodies (e.g., huMovl9 (M9346A)), pertuzumab, trastuzumab, bivatuzumab, sibrotuzumab, and rituximab (see, e.g., U.S. Patent Nos. 5,639,641 , 5,665,357; 7,342,1 10, 7,557,189; 7,342,1 10; and 8,119,787; PCT pubs WO 02/16,401 ; WO2004/103272; WO 2005/009369; WO 2007/024222; WO201 1/106528; and , U.S. Patent Publication Numbers 20120009181 ; 20060045877; 20060127407; and 20050118183; Pedersen et al., (1994) J. Mol. Biol. 235, 959-973, Roguska et al., (1994) Proceedings of the National Academy of Sciences, Vol 91, 969-973, supra, Roguska, M.A., et al., (1996) Protein Eng, 1996. 9(10): p. 895-904; Colomer et al., Cancer Invest., 19: 49-56 (2001), Heider et al., Eur. J. Cancer, 31A: 2385-2391 (1995), Welt et al., J Clin. Oncol. , 12: 1 193-1203 (1994), and Maloney et al., Blood, 90: 2188-2195 (1997)); and

-epitope-binding fragments of antibodies such as sFv, Fab, Fab', and F(ab') ₂ (Parham, J Immunol. 131 :2895-2902 (1983); Spring et al, J Immunol. 1 13:470-478 (1974); Nisonoff et al, Arch. Biochem. Biophys. 89:230-244 (I960)).

Additional cell-binding agents include other cell-binding proteins and polypeptides exemplified by, but not limited to:

- Ankyrin repeat proteins (DARPins; Zahnd et al., J Biol. Chem., 281, 46, 35167-35175, (2006); Binz, H.K., Amstutz, P. & Pluckthun, A. (2005) Nature Biotechnology, 23, 1257-1268) or ankyrin-like repeats proteins or synthetic peptides described, for example, in U.S. Patent Publication Number 20070238667; U.S. Patent No. 7,101,675; and

WO/2007/147213; WO/2007/062466);

-interferons (e.g. α, β, γ);

-lymphokines such as IL-2, IL-3, IL-4, 1L-6; -hormones such as insulin, TRH (thyrotropin releasing hormones), MSH (melanocyte-stimulating hormone), steroid hormones, such as androgens and estrogens;

-vitamins such as folic acid;

-growth factors and colony-stimulating factors such as EGF, TGF-a, G-CSF, M-CSF and GM-CSF (Burgess, Immunology Today 5: 155-158 (1984)); and

-transferrin (O'Keefe et al, J Biol. Chem. 260:932-937 (1985)).

[103] Particularly useful antibodies for use in any of the embodiments of the present invention include huC242, which binds CanAg; huB4, which binds CD 19; MF-T, which binds mesothelin; huDS6, which binds CA6; and huMy 9-6, which binds CD33. In some embodiments, the antibody is not an anti-FOLRl antibody (e.g., huMovl9 (M9346A)).

[104] Monoclonal antibody techniques allow for the production of specific cell- binding agents in the form of monoclonal antibodies. Particularly well known in the art are techniques for creating monoclonal antibodies produced by immunizing mice, rats, hamsters or any other mammal with the antigen of interest such as the intact target cell, antigens isolated from the target cell, whole virus, attenuated whole virus, and viral proteins such as viral coat proteins. Sensitized human cells can also be used. Another method of creating monoclonal antibodies is the use of phage libraries of sFv (single chain variable region), specifically human sFv {see, e.g., Griffiths et al, U.S. Patent No. 5,885,793; McCafferty et al, WO 92/01047; and Liming et al, WO 99/06587.)

[105] Selection of the appropriate cell-binding agent is a matter of choice that depends upon the particular cell population that is to be targeted, but in general monoclonal antibodies and epitope binding fragments thereof are preferred, if an appropriate one is available.

[106] For example, the monoclonal antibody My9 is a murine IgG _2a antibody that is specific for the CD33 antigen found on Acute Myeloid Leukemia (AML) cells (Roy et al. Blood 77:2404-2412 (1991)) and can be used to treat AML patients. Similarly, the monoclonal antibody anti-B4 is a murine IgGi, which binds to the CD 19 antigen on B cells (Nadler et al, J Immunol. 131 :244-250 (1983)) and can be used if the target cells are B cells or diseased cells that express this antigen such as in non-Hodgkin's lymphoma or chronic lymphoblastic leukemia. Similarly, the antibody N901 is a murine monoclonal IgGi antibody that binds to CD56 found on small cell lung carcinoma cells and on cells of other tumors of the neuroendocrine origin (Roy et al. J Nat. Cancer Inst. 88: 1136-1 145 (1996)), C242 antibody that binds to the CanAg antigen, Trastuzumab that binds to HER2/neu and anti-EGF receptor antibody.

[107] Additionally, GM-CSF, which binds to myeloid cells, can be used as a cell- binding agent to diseased cells from acute myelogenous leukemia. IL-2, which binds to activated T-cells, can be used for prevention of transplant graft rejection, for therapy and prevention of graft- versus-host disease, and for treatment of acute T-cell leukemia. MSH, which binds to melanocytes, can be used for the treatment of melanoma. Folic acid, which targets the folate receptor expressed on ovarian and other cancers is also a suitable cell- binding agent.

[108] Cancers of the breast and testes can be successfully targeted with estrogen (or estrogen analogues) or androgen (or androgen analogues), respectively, as cell-binding agents.

Drugs

[109] Drugs that can be used in the present invention include chemotherapeutic agents. "Chemotherapeutic agent" is a chemical compound useful in the treatment of cancer. Examples of chemotherapeutic agents include alkylating agents, such as thiotepa and cyclophosphamide (CYTOXAN™); alkyl sulfonates such as busulfan, improsulfan and piposulfan; aziridines, such as benzodopa, carboquone, meturedopa, and uredopa;

ethylenimines and methylamelamines including altretamine, triethylenemelamine, trietylenephosphoramide, triethylenethiophosphaoramide and trimethylolomelamine;

acetogenins (especially bullatacin and buUatacinone); a camptothecin (including the synthetic analogue topotecan); bryostatin; callystatin; CC-1065 (including its adozelesin, carzelesin and bizelesin synthetic analogues); cryptophycins (particularly cryptophycin 1 and cryptophycin 8); dolastatin; duocarmycin (including the synthetic analogues, KW-2189 and CBI-TMI); eleutherobin; pancratistatin; a sarcodictyin; spongistatin; nitrogen mustards such as chlorambucil, chlornaphazine, cholophosphamide, estramustine, ifosfamide,

mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, novembichin, phenesterine, prednimustine, trofosfamide, uracil mustard; nitrosureas such as carmustine, chlorozotocin, fotemustine, lomustine, nimustine, ranimustine; antibiotics, such as the enediyne antibiotics (e.g. calicheamicin, especially calicheamicin .gammal and calicheamicin theta I, see, e.g., Angew Chem Intl. Ed. Engl. 33: 183-186 (1994); dynemicin, including dynemicin A; an esperamicin; as well as neocarzino statin chromophore and related chromoprotein enediyne antiobiotic chromomophores), aclacinomysins, actinomycin, authramycin, azaserine, bleomycins, cactinomycin, carabicin, carminomycin, carzinophilin; chromomycins, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, doxorubicin (including morpholino-doxorubicin, cyanomorpholino-doxorubicin, 2-pyrroIino- doxorubicin and deoxydoxorubicin), epirubicin, esorubicin, idarubicin, marcellomycin, nitomycins, mycophenolic acid, nogalamycin, olivomycins, peplomycin, potfiromycin, puromycin, quelamycin, rodorubicin, streptonigrin, streptozocin, tubercidin, ubenimex, zinostatin, zorubicin; anti-metabolites, such as methotrexate and 5-fluorouracil (5-FU); folic acid analogues, such as denopterin, methotrexate, pteropterin, trimetrexate; purine analogs, such as fludarabine, 6-mercaptopurine, thiamiprine, thioguanine; pyrimnidine analogs such as, ancitabine, azacitidine, 6-azauridine, carmofur, cytarabine, dideoxyuridine, doxifluridine, enocitabine, floxuridine, 5-FU; androgens, such as calusterone, dromostanolone propionate, epitiostanol, mepitiostane, testolactone; anti-adrenals, such as aminoglutethimide, mitotane, trilostane; folic acid replenisher, such as frolinic acid; aceglatone; aldophosphamide glycoside; aminolevulinic acid; amsacrine; bestrabucil; bisantrene; edatraxate; defofamine; demecolcine; diaziquone; elfomithine; elliptinium acetate; an epothilone; etoglucid; gallium nitrate; hydroxyurea; lentinan; lonidamine; maytansinoids, such as maytansine and ansamitocins; mitoguazone; mitoxantrone; mopidamol; nitracrine; pentostatin; phenamet; pirarubicin; podophyllinic acid; 2-ethylhydrazide; procarbazine; PSK ^® ; razoxane; rhizoxin; sizofiran; spirogermanium; tenuazonic acid; triaziquone; 2, 2',2"-trichlorotriethylamine; trichothecenes (especially T-2 toxin, verracurin A, roridin A and anguidine); urethan;

vindesine; dacarbazine; mannomustine; mitobronitol; mitolactol; pipobroman; gacytosine; arabinoside ("Ara-C"); cyclophosphamide; thiotepa; taxoids, e.g. paclitaxel (TAXOL ^® , Bristol-Myers Squibb Oncology, Princeton, N.J.) and doxetaxel (TAXOTERE ^® , Rhone- Poulenc Rorer, Antony, France); chlorambucil; gemcitabine; 6-thioguanine; mercaptopurine; methotrexate; platinum analogs such as cisplatin and carboplatin; vinblastine; platinum; etoposide (VP- 16); ifosfamide; mitomycin C; mitoxantrone; vincristine; vinorelbine;

navelbine; novantrone; teniposide; daunomycin; aminopterin; xeloda; ibandronate; CPT-11 ; topoisomerase inhibitor RFS 2000; difluoromethylomithine (DMFO); retinoic acid;

capecitabine; and pharmaceutically acceptable salts, acids or derivatives of any of the above. Also included in this definition are anti-hormonal agents that act to regulate or inhibit hormone action on tumors, such as anti-estrogens including for example tamoxifen, raloxifene, aromatase inhibiting 4(5)-imidazoles, 4-hydroxytamoxifen, trioxifene, keoxifene, LY1 17018, onapristone, and toremifene (Fareston); and anti-androgens, such as flutamide, nilutamide, bicalutamide, leuprolide, and goserelin; siRNA and pharmaceutically acceptable salts, acids or derivatives of any of the above. Other chemotherapeutic agents that can be used with the present invention are disclosed in US Publication No. 20080171040 or US Publication No. 20080305044 and are incorporated in their entirety by reference.

[110] In a preferred embodiment, chemotherapeutic drugs are essentially small molecule drugs. A "small molecule drug" is broadly used herein to refer to an organic, inorganic, or organometallic compound that may have a molecular weight of for example 100 to 1500, more suitably from 120 to 1200, favorably from 200 to 1000, and typically having a molecular weight of less than about 1000. Small molecule drugs of the invention encompass oligopeptides and other biomolecules having a molecular weight of less than about 1000. Small molecule drugs are well characterized in the art, such as in WO05058367A2, European Patent Application Nos. 85901495 and 8590319, and in U.S. Patent No. 4,956,303, among others and are incorporated in their entirety by reference.

[Ill] Preferable small molecule drugs are those that allow for linkage to the cell- binding agent. The invention includes known drugs as well as those that may become known. Especially preferred small molecule drugs include cytotoxic agents.

[112] The cytotoxic agent may be any compound that results in the death of a cell, or induces cell death, or in some manner decreases cell viability, wherein each cytotoxic agent comprises a thiol moiety.

[113] Preferred cytotoxic agents are maytansinoid compounds, taxane compounds,

CC-1065 compounds, daunorubicin compounds and doxorubicin compounds,

pyrrolobenzodiazepine dimers, calicheamicins. Auristatins and analogues and derivatives thereof, some of which are described below.

[114] Other cytotoxic agents, which are not necessarily small molecules, such as siRNA, are also encompassed within the scope of the instant invention. For example, siRNAs can be linked to the crosslinkers of the present invention by methods commonly used for the modification of oligonucleotides (see, for example, US Patent Publications

20050107325 and 20070213292). Thus the siRNA in its 3' or 5'-phosphoromidite form is reacted with one end of the crosslinker bearing a hydroxyl functionality to give an ester bond between the siRNA and the crosslinker. Similarly reaction of the siRNA phosphoramidite with a crosslinker bearing a terminal amino group results in linkage of the crosslinker to the siRNA through an amine. siRNA are described in detail in U.S. Patent Publication Numbers: 20070275465, 20070213292, 20070185050, 20070161595, 20070054279, 20060287260, 20060035254, 20060008822, 20050288244, 20050176667, which are incorporated herein in their entirety by reference.

Maytansinoids

[115] Maytansinoids that can be used in the present invention are well known in the art and can be isolated from natural sources according to known methods or prepared synthetically according to known methods.

[116] Examples of suitable maytansinoids include maytansinol and maytansinol analogues. Examples of suitable maytansinol analogues include those having a modified aromatic ring and those having modifications at other positions.

[117] Specific examples of suitable analogues of maytansinol having a modified aromatic ring include:

(1) C-19-dechloro (U.S. Patent No. 4,256,746) (prepared by LAH reduction of ansamitocin P2);

(2) C-20-hydroxy (or C-20-demethyl) +/-C-19-dechloro (U.S. Patent Nos. 4,361,650 and 4,307,016) (prepared by demethylation using Streptomyces or Actinomyces or dechlorination using LAH); and

(3) C-20-demethoxy, C-20-acyloxy (-OCOR), +/-dechloro (U.S. Patent No. 4,294,757) (prepared by acylation using acyl chlorides).

[118] Specific examples of suitable analogues of maytansinol having modifications of other positions include:

(1) C-9-SH (U.S. Patent No. 4,424,219) (prepared by the reaction of maytansinol with H2S or P2S5);

(2) C-14-alkoxymethyl (demethoxy/CH20 ) (U.S. Patent No. 4,331,598);

(3) C-14-hydroxymethyl or acyloxymethyl (CH20H or CH20Ac) (U.S. Patent No. 4,450,254) (prepared from Nocardia);

(4) C-15-hydroxy/acyloxy (U.S. Patent No. 4,364,866) (prepared by the conversion of maytansinol by Streptomyces); (5) C-15-methoxy (U.S. Patent Nos. 4,313,946 and 4,315,929) (isolated from Trewia nudiflora);

(6) C-18-N-demethyl (U.S. Patent Nos. 4,362,663 and 4,322,348) (prepared by the demethylation of maytansinol by Streptomyces); and

(7) 4,5-deoxy (U.S. Patent No. 4,371,533) (prepared by the titanium trichloride/LAH reduction of maytansinol).

[119] The synthesis of thiol-containing maytansinoids useful in the present invention is fully disclosed in U.S. Patent Nos. 5,208,020, 5,416,064, and U. S. Patent Application No. 20040235840.

[120] Maytansinoids with a thiol moiety at the C-3 position, the C-14 position, the

C-15 position or the C-20 position are all expected to be useful. The C-3 position is preferred and the C-3 position of maytansinol is especially preferred. Also preferred are an vV-methyl- alanine-containing C-3 thiol moiety maytansinoid, and an N-methyl-cysteine-containing C-3 thiol moiety maytansinoid, and analogues of each.

[121] Specific examples of N-methyl-alanine-containing C-3 thiol moiety maytansinoid derivatives useful in the present invention are represented by the formulae Ml, M2, M3, M6 and M7.

Ml

wherein:

/ is an integer of from 1 to

may is a maytansinoid.

wherein:

Ri and R ₂ are H. C3¾ or CI l U, and may be the same or different; m is 0, 1 , 2 or 3 ; and

may is a rnaytansinoid.

wherein:

n is an integer of from 3 to 8; and

may is a mavlansinoid.

wherein:

/ is K 2 or 3;

Yo is CI or H; and X ₃ is H or CH ₃ .

M7

wherein:

Rj, R ₂ , R ₃ , } are H, CH ₃ or CH ₂ CH ₃ , and may be the same or different; m is 0, 1, 2 or 3; and

may is a maytansinoid.

[122] Specific examples of N-methyl-cysteine-containing C-3 thiol moiety maytansinoid derivatives useful in the present invention are represented by the formulae M4 and M5.

y wherein:

o is 1, 2 or 3;

p is an integer of 0 to 10; and

may is a maytansinoid. wherein:

o is 1 , 2 or 3 ;

q is an integer of from 0 to 10;

Yo is CI or H; and

X ₃ is H or CH ₃ .

Preferred maytansinoids are those described in U.S. Patent Nos. 5,208,020;

5,416,064; 6,333.410; 6,441 ,163; 6,716,821 ; RE39J 51 and 7,276,497. Especially preferred is the maytansinoid DM4 (N(2')-deaeetyl-N2 ^, ~(4-mercapto-4-methyl-l-oxopentyi)- maytansine which is described in detail in U. S.P.N. 7,276,497.

DM4

Taxanes

[123] The cytotoxic agent according to the present invention may also be a taxane.

[124] Taxanes that can be used in the present invention have been modified to contain a thiol moiety. Some taxanes useful in the present invention have the formula Tl shown below:

[125] Four embodiments of these novel taxanes are described below.

[126] in embodiments (1), (2), (3), and (4), Ri, Ri ', and R," are the same or different and are H, an electron withdrawing group, such as F, N0 ₂ , CN, CI, CHF ₂ , or CF ₃ or an electron donating group, such as -OCH3, -OCH ₂ CH ₃ , -NR R ₈ , -OR9, wherein R ₇ and R ₈ are the same or different and are linear, branched, or cyclic alkyl groups having 1 to 10 carbon atoms or simple or substituted aryl having 1 to 10 carbon atoms. Preferably the number of carbon atoms for R ₇ and R ₈ is 1 to 4. Also, preferably R ₇ and R ₈ are the same. Examples of preferred -NR ₇ R ₈ groups include dimethyl amino, diethyl amino, dipropyl amino, and dibutyl amino, where the butyl moiety is any of primary, secondary, tertiary or isobutyl. R9 is linear, branched or cyclic alkyl having 1 to 10 carbon atoms.

[127] Ri preferably is OCH ₃ , F, N0 ₂ , or CF ₃ .

[128] Also preferably, Ri is in the meta position and Ri ' and Rj " are H or OCH ₃ .

[129] R ₂ in embodiments (1), (2) and (4), is H, heterocyclic, a linear, branched, or cyclic ester having from 1 to 10 carbon atoms or heterocyclic, a linear, branched, or cyclic ether having from 1 to 10 carbon atoms or a carbamate of the formula -CONRioRn, wherein Rio and Rn are the same or different and are H, linear alkyl having from 1 -6 carbon atoms, branched, or cyclic alkyl having 3 to 10 atoms or simple or substituted aryl having 6 to 10 carbon atoms. For esters, preferred examples include -COCH ₂ CH ₃ and -COCH ₂ CH ₂ CH ₃ . For ethers, preferred examples include -CH ₂ CH ₃ and -CH ₂ CH ₂ CH ₃ . For carbamates, preferred examples include -CONHCH ₂ CH _3, -CONHCH ₂ CH ₂ CH ₃ , -CO-morpholino, -CO- piperazino, -CO-piperidino, or -CO-N-methylpiperazino.

[130] R ₂ in embodiment (3), is a thiol-containing moiety. [131] R ₃ in embodiments (1), (3) and (4), is aryl, or is linear, branched or cyclic alkyl having 1 to 10 carbon atoms, preferably -CH ₂ CH(CH ₃ ) ₂ .

[132] R ₃ in embodiment (2), is -CH=C(CH ₃ ) ₂

[133] R ₄ in all four embodiments, is -OC(CH ₃ ) ₃ or -C ₆ H ₅ .

[134] R ₅ in embodiments (1) and (2), is a thiol-containing moiety and R ₆ has the same definition as above for R ₂ for embodiments (1), (2) and (4).

[135] R ₅ and R6 in embodiment (3), are the same or different, and have the same definition as above for R ₂ for embodiments (1), (2) and (4).

[136] R ₅ in embodiment (4), has the same definition as above for R ₂ for

embodiments (1), (2) and (4) and R ₆ is a thiol moiety.

[137] The preferred positions for introduction of the thiol-containing moiety are R ₂ and R ₅ , with R ₂ being the most preferred.

[138] The side chain carrying the thiol moiety can be linear or branched, aromatic or heterocyclic. One of ordinary skill in the art can readily identify suitable side chains.

Specific examples of thiol moieties include -(CH ₂ ) _n SH, -CO(CH ₂ ) _n SH, -(CH ₂ ) _n CH(CH ₃ )SH, -CO(CH ₂ ) _n CH(CH ₃ )SH, -(CH ₂ ) _n C(CH ₃ ) ₂ SH, -CO(CH ₂ ) _n C(CH ₃ ) ₂ SH, -CONR ₁₂ (CH ₂ ) _n SH, -CONR ₁₂ (CH ₂ ) _n CH(CH ₃ )SH, or -CONR ₁₂ (CH ₂ ) _n C(CH ₃ ) ₂ SH, -CO-morpholino-XSH, -CO- piperazino-XSH, -CO-piperidino-XSH, and -CO-N-methylpiperazino-XSH wherein

X is a linear alkyl or branched alkyl having 1-10 carbon atoms.

R ₁₂ is a linear alkyl, branched alkyl or cyclic alkyl having 1 to 10 carbon atoms, or simple or substituted aryl having from 1 to 10 carbon atoms or heterocyclic, and can be H, and

n is an integer of 0 to 10.

[139] Examples of linear alkyls include methyl, ethyl, propyl, butyl, pentyl and hexyl.

[140] Examples of branched alkyls include isopropyl, isobutyl, sec-butyl, tert.-butyl, isopentyl and 1 -ethyl -propyl.

[141] Examples of cyclic alkyls include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.

[142] Examples of simple aryls include phenyl and naphthyl. [143] Examples of substituted aryls include aryls such as those described above substituted with alkyl groups, with halogens, such as CI, Br, F, nitro groups, amino groups, sulfonic acid groups, carboxylic acid groups, hydroxy groups or alkoxy groups.

[144] Examples of heterocyclics are compounds wherein the heteroatoms are selected from O, N, and S, and include morpholino, piperidino, piperazino, N- methylpiperazino, pyrrollyl, pyridyl, furyl and thiophene.

[145] The taxanes having a thiol moiety can be synthesized according to known methods. The starting material for the synthesis is the commercially available 10- deacetylbaccatin III. The chemistry to introduce various substituents is described in several publications (Ojima et al, J Med. Chem. 39:3889-3896 (1996); Ojima et al, J Med. Chem. 40:267-278 (1997); Ojima et al., Proc. Natl. Acad 5 ^* d. , 96:4256-4261 (1999); U.S. Patent No. 5,475,011 and U.S. Patent No. 5,811,452).

[146] The substituent Ri on the phenyl ring and the position of the substituent Ri can be varied until a compound of the desired toxicity is obtained. Furthermore, the degree of substitution on the phenyl ring can be varied to achieve a desired toxicity. That is, the phenyl ring can have one or more substituents (e.g., mono-, di-, or tri-substitution of the phenyl ring) which provide another means for achieving a desired toxicity. One of ordinary skill in the art can determine the appropriate chemical moiety for Ri and the appropriate position for Ri using only routine experimentation.

[147] For example, electron withdrawing groups at the meta position increase the cytotoxic potency, while substitution at the para position is not expected to increase ihe potency as compared to the parent taxane. Typically, a few representative taxanes with substituents at the different positions (ortho, meta and para) will be initially prepared and evaluated for in vitro cytotoxicity.

[148] The thiol moiety can be introduced at one of the positions where a hydroxyl group already exists. The chemistry to protect the various hydroxyl groups, while reacting the desired one, has been described previously (see, for example, the references cited supra). The substituent is introduced by simply converting the free hydroxyl group to a

disulfide-containing ether, a disulfide-containing ester, or a disulfide-containing carbamate. This transformation is achieved as follows. The desired hydroxyl group is deprotonated by treatment with the commercially-available reagent lithium hexamethyldisilazane (1.2 equivalents) in tetrahydrofuran at -40°C as described in Ojima et al. (1999), supra. The resulting alkoxide anion is then reacted with an excess of a dihalo compound, such as dibromoethane, to give a halo ether. Displacement of the halogen with a thiol (by reaction with potassium thioacetate and treatment with mild base or hydroxylamine) will provide the desired thiol-containing taxane.

[149] Alternatively, the desired hydroxyl group can be esterified directly by reaction with an acyl halide, such as 3-bromopropionyl chloride, to give a bromo ester. Displacement of the bromo group by treatment with potassium thioacetate and further processing as described above will provide the thiol-containing taxane ester. Preferred taxoids are those described in U.S. Patent Nos. 6,340,701 ; 6,372,738; 6.436,931 ; 6,596,757; 6,706,708;

7,008,942; 7,217,819 and 7,276,499.

CC-1065 analogues

150] The cytotoxic agent according to the present invention may also be a CC-1065 According to the present invention, the CC-1065 analogues contain an A subunit and a B or a B-C subunit. The A subunits are CPI (cyclopropapyrroloindole unit) in its natural closed cyclopropyl form or in its open chloromethyl form, or the closely related CBI unit (cyclopropylbenzindole unit) in the closed cyclopropyl form or the open

chloromethyl form. The B and C subunits of CC-1065 analogues are very similar and are 2- carboxy-indole and 2-carboxy-benzofuran derivatives. For activity, the analogues of CC- 1065 need at least one such 2-carboxy-indole subunit or 2-carboxy-benzofuran subunit, although two subunits (i.e., B-C) render the analogue more potent. As is obvious from the natural CC-1065 and from the analogues published {e.g., Warpehoski et al, J Med. Chem. 31 :590-603 (1988), D. Boger et al., J Org. Chem; 66; 6654-6661 , 2001 ; U. S. Patent Nos 5,739,350; 6,060,608; 6.310.209), the B and C subunits can also carry different substituents at different positions on the indole or benzofuran rings.

[152] CC-1065 analogues containing a thiol moiety can be any of the following A subunits of the formulae A-l {CPI (Cyclopropyl form)}, A-2 {CPI (Chloromethyl form)}, A- 3 {CBI (Cyclopropyl form)}, and A-4 {CBI (Chloromethyl form)} covalently linked via an amide bond from the secondary amino group of the pyrrole moiety of the A subunit to the C- 2 carboxy group of either a B subunit of the formula F-l or a B-C subunit of the formulae F-

3 or F-7. A subunits

wherein each Wi and W ₂ may be the same or different and may be O or NH; and

wherein, in Formula F-l R4 is a thiol moiety, in Formula F-3 one of R or R is a thiol moiety, in Formula F-7 one of R' or R4 is a thiol-containing moiety; when R or R' is a thiol moiety, then Ri to R ₆ , which may be the same or different, are hydrogen, C\ -C ₃ linear alkyl, methoxy, hydroxyl, primary amino, secondary amino, tertiary amino, or amido; and when R4 is a thiol moiety, R, Ri, R ₂ , R ₃ , R4, R ₅ and R ₆ , which may be the same or different, are hydrogen, C _\ -C3 linear alkyl, methoxy, hydroxyl, primary amino, secondary amino, tertiary amino, or amido, and R' is NH ₂ , alkyl, O-alkyl, primary amino, secondary amino, tertiary amino, or amido. In addition, the chlorine atom in A-2 and A-4 subunits can be replaced with another suitable halogen. [153J In a preferred embodiment, R and R' are thiol moieties and Ri and R ₂ are each hydrogen. In another preferred embodiment, R and R' are thiol moieties and R _\ to Re are each hydrogen.

[154] In an especially preferred embodiment, R or R4 is -NHCO(CH2)/SH,

-NHCOC ₆ H ₄ (CH ₂ )/SH, or -0(CH ₂ ) _/ SH, and R' is -(CH ₂ ) SH, -NH(CH ₂ ),SH or -0(CH ₂ )/SH wherein / is an integer of 1 to 10.

[155] Examples of primary amines include methyl amine, ethyl amine and isopropyl amine.

[156] Examples of secondary amines include dimethyl amine, diethylamine and ethylpropyl amine.

[157] Examples of tertiary amines include trimethyl amine, triethyl amine, and ethyl-isopropyl-methyl amine.

[158] Examples of amido groups include N-methylacetamido, N-methyl- propionamido, N-acetamido, and N-propionamido.

[159] Examples of alkyl represented by R', when R' is not a linking group, include

C1-C5 linear or branched alkyl.

[160] Examples of O-alkyl represented by R' when R' is not a linking group, include compounds where the alkyl moiety is a C ₁ -C5 linear or branched alkyl.

[161] The above-described CC-1065 analogues may be isolated from natural sources and methods for their preparation, involving subsequent modification, synthetic preparation, or a combination of both, are well-described (see, e.g. , U.S. patent nos. 5,475,092, 5,585,499 and 5,846,545). Preferred CC-1065 analogs are those described in U.S. Patent Nos.

5,475,092; 5,595,499; 5,846,545; 6,534,660; 6,586,618; 6,756,397 and 7,049,316

Daunorub icin/Doxorub ic in A nalogues

[162] The cytotoxic agent according to the present invention may also be a daunorubicin analogue or a doxorubicin analogue.

[163] The daunorubicin and doxorubicin analogues of the present invention can be modified to comprise a thiol moiety.

[164] The modified doxorubicin/daunorubicin analogues useful in the present invention have the formula Dl shown below:

wherein,

X is H or OH;

Y is O or NR ₂ , wherein R ₂ is linear or branched alkyl having 1 to 5 carbon atoms;

R is a thiol moiety, H, or liner or branched alkyl having 1 to 5 carbon atoms; and

R' is a thiol moiety, H, or -OR), wherein R] is linear or branched alkyl having 1 to 5 carbon atoms;

provided that R and R' are not thiol moieties at the same time.

[165] In a preferred embodiment, NR ₂ is NCH ₃ . In another preferred embodiment,

R' is -O.

[166] In an especially preferred embodiment, the thiol moiety is -(CH ₂ ) _n SH,

-0(CH ₂ ) _n SH, -(CH ₂ ) _n CH(CH ₃ )SH, -0(CH ₂ ) _n CH(CH ₃ )SH, -(CH ₂ ) _n C(CH ₃ ) ₂ SH, or

-0(CH ₂ ) _n C(CH ₃ ) ₂ SH, wherein n is an integer of 0 to 10.

[167] Examples of the linear or branched alkyl having 1 to 5 carbon atoms, represented by R, Ri, and R _2i include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert.-butyl, and pentyl, in any of its eight isomeric arrangements.

[168] Ri and R ₂ preferably are methyl.

[169] Examples of linear alkyls include methyl, ethyl, propyl, butyl, pentyl and hexyl.

[170] Examples of branched alkyls include isopropyl, isobutyl, sec. -butyl, tert.-butyl, isopentyl and 1 -ethyl -propyl.

[171] When either R or R' is not a linking group, the substituent in that position can be varied until a compound of the desired toxicity is obtained. High toxicity is defined as having an ICso towards cultured cancer cells in the range of 1 x 10 ^",2 to 1 x 1(T ⁹ M, upon a 72 hour exposure time. Representative examples of substituents are H, alkyl, and O-alkyl, as described above. One of ordinary skill in the art can determine the appropriate chemical moiety for R and R' using only routine experimentation.

[172] For example, methyl and methoxy substituents are expected to increase the cytotoxic potency, while a hydrogen is not expected to increase the potency as compared to the parent daunorubicin analogues with substituents at the different positions will be initially prepared and evaluated for in vitro cytotoxicity.

[173] The modified doxorubicin/daunorubicin analogues of the present invention, which have a thiol moiety, are described in WO 01/38318. The modified

doxorubicin/daunorubicin analogues can be synthesized according to known methods {see, e.g., U.S. Patent No. 5,146,064).

[174] Auristatin include auristatin E, auristatin EB (AEB), auristatin EFP (AEFP), monomethyl auristatin E (MMAE) and are described in U.S. Patent No. 5,635,483, Int. J Oncol 15:367-72 (1999); Molecular Cancer Therapeutics, vol. 3, No. 8, pp. 921-932 (2004); U.S. Application Number 1 1/134826. U.S. Patent Publication Nos. 20060074008,

2006022925.

[175] The cytotoxic agents according to the present invention include

pyrrolobenzodiazepine dimers that are known in the art (US Patent Nos 7,049,311;

7.067.51 1 ; 6,951 ,853; 7,189,710; 6,884,799; 6,660,856.

Analogues and derivatives

[176] One skilled in the art of cytotoxic agents will readily understand that each of the cytotoxic agents described herein can be modified in such a manner that the resulting compound still retains the specificity and/or activity of the starting compound. The skilled artisan will also understand that many of these compounds can be used in place of the cytotoxic agents described herein. Thus, the cytotoxic agents of the present invention include analogues and derivatives of the compounds described herein.

[177] Cancer therapies and their dosages, routes of administration and recommended usage are known in the art and have been described in such literature as the Physician's Desk Reference (PDR). The PDR discloses dosages of the agents that have been used in treatment of various cancers. The dosing regimen and dosages of these aforementioned chemotherapeutic agents and conjugates that are therapeutically effective will depend on the particular cancer being treated, the extent of the disease and other factors familiar to the physician of skill in the art and can be determined by the physician. For example, the 2006 edition of the Physician's Desk Reference discloses that Taxotere (see p. 2947) is an inhibitor of tubulin depolymerization; Doxorubicin (see p 786), Doxil (see p 3302) and oxaliplatin (see p 2908) are DNA interacting agents, Irinotecal (see p. 2602) is a Topoisomerase I inhibitor, Erbitux (see p 937) and Tarceva (see p 2470) interact with the epidermal growth factor receptor. The contents of the PDR are expressly incorporated herein in their entirety by reference. One of skill in the art can review the PDR, using one or more of the following parameters, to determine dosing regimens and dosages of the chemotherapeutic agents and conjugates, which can be used in accordance with the teachings of this invention. These parameters include:

1. Comprehensive index

a) by Manufacturer

b) Products (by company's or trademarked drug name)

c) Category index (for example, "antihistamines", "DNA alkylating agents" taxanes etc.)

d) Generic/chemical index (non-trademark common drug names)

2. Color images of medications

3. Product information, consistent with FDA labeling

a) Chemical information

b) Function/action

c) Indications & Contraindications

d) Trial research, side effects, warnings

[178] The antibody drug conjugates of the present invention, comprising a cell binding agent, DM4, and a linker having at least one charged group, are especially useful to treat a range of disorders because they can be administered at a dose/frequency that is higher than could be administered with an antibody drug conjugate having the same cell binding agent, DM4, but a linker without at least one charged group. The higher dose/frequency is achievable because the inclusion of a charged group in the linker reduces the ocular toxicity associated with the antibody drug conjugate. In one embodiment, the disorder is a proliferative disorder such as cancer. [179] The present invention further provides methods for inhibiting tumor growth using the antibody drug conjugates described herein. In certain embodiments, the method of inhibiting the tumor growth comprises contacting the cell with an antibody drug conjugate in vitro. In some embodiments, tumor cells are isolated from a patient sample such as, for example, a tissue biopsy, pleural effusion, or blood sample and cultured in medium to which is added an antibody drug conjugate to inhibit tumor growth.

[180] In some embodiments, the method of inhibiting tumor growth comprises contacting the tumor or tumor cells with the antibody drug conjugate in vivo. In certain embodiments, contacting a tumor or tumor cell with an antibody drug conjugate is undertaken in an animal model, in some embodiments, the antibody drug conjugate is administered at the same time or shortly after introduction of tumorigenic cells into the subject to prevent tumor growth. In some embodiments, the antibody drug conjugate is administered as a therapeutic after the tumorigenic cells have grown to a specified size.

[181] In certain embodiments, the method of inhibiting tumor growth comprises administering to a subject a therapeutically effective amount of an antibody drag conjugate. In certain embodiments, the subject is a human. In certain embodiments, the subject has a tumor or has had a tumor removed,

[182] In certain embodiments, the tumor is a tumor selected from the group consisting of brain tumor, colorectal tumor, pancreatic tumor, lung tumor (e.g., SCLC or SCLC), ovarian tumor, liver tumor, breast tumor, kidney tumor, prostate tumor, gastrointestinal tumor, melanoma, cervical tumor, bladder tumor, glioblastoma, and head and neck tumor. In certain embodiments, the tumor is an ovarian tumor.

Charged or pro-charged Imkers and reduced ocular toxjcitY

[183] In vivo dosing of antibody drug conjugates (ADCs) involves an analysis of the pharmacokinetic profiles of the conjugates (with resulting therapeutic benefit) balanced against possible side effects induced by administration. As has been shown previously, antibody drug conjugates containing DM4 and non-charged linkers, such as SPDB, can cause ocular toxicity. In general, the maximum tolerated dose for antibody drug conjugates containing DM4 and non-charged linkers is approximately 160 mg/m ² (4.3 mg/kg), administered at a frequency of every 3 weeks. (A. Younes, et al 5 ' Annual Meeting of the American Society of Hematology, 585, December 7, 2009; L.W. Goff, et al , Journal of Clinical Oncology, 2009 ASCO Annual Meeting Proceedings (Post-Meeting Edition), 27, No. 15S (May 20 Supplement), 2009: el5625). Thus, the invention further relates to reducing the incidence of toxicity associated with DM4-containing antibody drug conjugates by inclusion of charged or pro-charged linkers in the conjugates. Inclusion of charged or pro- charged linkers allows for higher administration and/or greater frequency of dosing.

[184] As described in further detail below, linkers can contribute to the ocular toxicity of antibody drug conjugates seen in both a rabbit model system and in humans, especially at high doses of administration. Severity of toxicity is generally reported on a 4 grade scale and can include criteria as outlined below:

Central Nervous System

Grade 1

Visual

[185] Thus, as described in Examples 8, 9, and 10 below, antibody drug conjugates that comprise non-charged linkers and DM4 maytansanoids induce ocular toxicity when administered in a rabbit model system, or in humans. While more prevalent in higher dose administrations, ocular toxicity also occurs in dosages as low as about 4 mg/kg. The present invention overcomes the ocular toxicity issues of the previous antibody drug conjugates by introducing a linker comprising at least one charged group into the antibody-DM4 conjugate.

[186] Therefore, the invention provides a method to overcome ocular toxicity of

DM4-containing antibody drug conjugates at a range of dosages. In certain embodiments, antibody drug conjugates comprising a linker containing at least one charged group are administered at a dose of at least about 4 mg/kg (148 mg/m ² ). In another embodiment, such conjugates are administered at a dose of between about 4 mg/kg and about 16 mg kg (148

2 2

mg/m - 529 mg/m ). In another embodiment, such conjugates are administered at a dose of between about 4 mg/kg and about 8 mg/kg (148 mg/m ² - 296 mg/m ² ). In another embodiment, such conjugates are administered at a dose of between about 5 mg/kg and 6

9

mg/kg (185 mg/m - 216 mg/m ). In another embodiment, such conjugates are administered

9 9

at a dose of between about 6 mg/kg and 8 mg/kg (216 mg/m ~~ 296 mg/m ). In another embodiment, such conjugates are administered at a dose of between about 6 mg/kg and 7

9 9

mg/kg (216 mg/m - 259 mg/m ). In another embodiment, such conjugates are administered

9 9

at a dose of between about 7 mg/kg and 8 mg/kg (259 mg/m - 296 mg/m ). In a further embodiment such conjugates are administered at a dose of between about 4 mg/kg and 6 mg/kg (148 mg/m ² - 216 mg/m ² ). In another embodiment such conjugates are administered

9 9

at a dose of between about 4 mg/kg and 5 mg/kg (148 mg/m - 185 mg/m ). In another embodiment, such conjugates are administered at a dose of about 4.3 mg/kg (160 mg/m ² ).

[187] In another embodiment, antibody drug conjugates comprising a linker containing at least one charged group are administered at a greater frequency than conjugates which do not comprise a charged linker. In one embodiment, the antibody drug conjugates comprising a charged linker are administered at a frequency of at least once every two weeks. In another embodiment, the conjugate is administered at a frequency of at least once every three weeks. In a further embodiment, the conjugate is administered at a frequency of at least once every four weeks. In a further embodiment, the conjugate is administered at a frequency of once every two to four weeks or once every three weeks.

[188] As described above, the present invention is based on the discovery that inclusion of a charged group in the linker of the ADC decreases the incidence of ocular toxicity as compared to an ADC that contains a linker which is not charged. In one embodiment, the charged group is selected from the group consisting of: sulfonate, phosphate, carboxyl and quaternary amine. In a further embodiment the charged group is a sulfonate. These charged groups are introduced into a variety of linkers including N- succinimidyl 4-(2-pyridyldithio)-2-sulfopentanoate (sulfo-SPP); N-succinimidyl 4-(2- pyridyldithio)-2-sulfobutanoate (sulfo-SPDB); and N-sulfosuccinimidyl 4-(maleimidomethyl) cyclohexanecarboxylate (sulfoSMCC). In one embodiment, the linker is sulfo-SPDB. [189] In another embodiment, the invention is directed to a method of increasing the amount of an antibody drug conjugate tolerated by a subject by substituting a charged or pro- charged linker for a non-charged linker.

[190] All references cited herein and in the examples that follow are expressly incorporated by reference in their entireties.

EXAMPLES

[191] The invention will now be described by reference to non-limiting examples.

Unless otherwise specified, all percents and ratios are by volume.

Example 1: Materials and Methods

Methyl 2-(acetylthio)-4-bromobutanoate

[192] 10.0 g (38.4 mmol) of methyl 2,4-dibromobutanoate in 100 ml of dry THF at

20°C was added drop wise the mixture of 2.75 ml (38.5 mmol) of thiolacetic acid in 8.5 ml (48.9 mmol) of DIPEA and 50 ml of dry THF in 1.5 hour. After stirring overnight at -20°C then 0°C for 2 hours under Ar, the mixture was concentrated, diluted with EtAc/Hexane, washed with 1.0 M NaH ₂ P0 ₄ , dried over MgS0 ₄ , filtered, evaporated, and Si0 ₂ chromatographic purification (1 :12 to 1 : 10 EtAc/Hexane) to afford 9.5 g (96%) of the title compound. 1H NMR (CDC13) 4.38 (1H, t, J = 7.1Hz), 3.74 (s, 3H), 3.40 (m, 2H), 2.57 ~ 2.47 (m, 1H), 2.37 (s, 3H), 2.36 ~ 2.21 (m, 1H); 13C NMR 193.24, 171.36, 53.15, 44.45, 34.67, 30.46, 29.46; MS m/z+ 276.9 (M+Na), 278.9 (M+2+Na) -Bromo- 1 -methoxy- 1 -oxobutane-2-sulfonic acid [193] 9.2 g (36.3 mmol) of methyl 2-(acetylthio)-4-bromobutanoate in 80 ml of acetic acid was added 40 ml of hydrogen peroxide (35% in water). The mixture was stirred overnight, then evaporated, diluted with water, neutralized with NaHC0 ₃ , washed with 1 : 1 Hexane/EtAc. The aqueous solution was evaporated, dissolved in methanol, concentrated, and crystallized with methanol/toluene to afford 8.6 g (90% yield) of the title compound, m.p. = 288- 293 (decomp); 1H NMR (D20) 4.12 (dd, 1H, J ^ 4.8, 9.3 Hz), 3.83 (s, 3H), 3.64 (m, 1H), 3.53 (m, 1H), 2.54 (m, 2H); 13C NMR 172.16, 66.73, 55.66, 33.39, 32.70; MS m/z- 260.8 (M-l). -(Acetylthio)- 1 -methoxy- 1 -oxobutane-2-sulfonic acid

[194] 5.0 g (19.2 mmol) of 4-bromo-l -methoxy- 1 -oxobutane-2-sulfonic acid in 100 ml of THF was added 3.0 ml of thioacetic acid and 9.0 ml of DIPEA in 100 ml of THF. The mixture was stirred overnight then refluxed at 70°C for 1 hr, evaporated and co-evaporated with 3 x 100 ml of water after being neutralized to pH 7 with NaHC0 ₃ . The mixture was redissolved in methanol, filtered through celite, concentrated and purified with Si0 ₂ chromatography eluted with CH ₃ OH/CH ₂ Cl ₂ /HCOOH 37.5:250: 1 to 50:250:1) to afford 4.4 g (90% yield) of the title compound. 1H NMR(D20) 3.95 (dd, 1H, J = 4.1 , 10.3 Hz), 3.83 (s, 3H), 3.74 (m, 2H), 3.22 (dd, 2H, J - 7.4, 14.9 Hz), 2.39 (s, 3H); 13C NMR 203.88, 172.91, 67.32, 56.17, 29.04, 20.61 ; MS m/z- 254.8 (M-H)

4-((5-nitropyridin-2-yl)disulfanyl)-2-sulfobutanoic acid

[195] 3.0 g (11.7 mmol) of 4-(Acetylthio)-l -methoxy- 1 -oxobutane-2-sulfonic acid in 100 ml of water was added 50 ml of 3 M NaOH. After being stirred under Ar for 3 h, the mixture was neutralized with 1 M H ₂ P0 ₄ to pH 7.2 under Ar. The mixture was added dropwise to the solution of 10.0 g (32.2 mmol) of l,2-bis(5-nitropyridin-2-yl)disulfane in 200 ml of DMA. After being stirred for 4 h under Ar, the mixture was concentrated, diluted with water, filtered, evaporated and purified with C-18 4.0 x 20 cm column eluted with water/methanol (95:5) to afford 3.1 g (75% yield) of the title compound, m.p. = 288 ~ 291°C (decomp.) 1H NMR (DMF-d7) 9.29 (d, 1H, J = 2.2 Hz), 8.63 (dd, 1H, J = 2.7, 8.9 Hz), 8.17 (d, 1H, J - 8.9 Hz), 3.73 (t, 1H, J = 7.2 Hz), 3.22 ~ 3.17 (m, 1H), 3.15 - 3.10 (m, 1H), 2.41 ~ 2.33 (m, 2H); 13C NMR 170.92, 169.10, 146.04, 143.67, 133.65, 120.72, 64.22, 37.82, 29.26; MS m/z- 352.8 (M-H). l-(2,5-dioxopyrrolidin-l-yloxy)-4-((5-nitropyridin-2-yl)disu lfanyl)-l -oxobutane-2-sulfonic id

[196] 220 mg (0.62 mmol) of 4-((5-nitropyridin-2-yl)disulfanyl)-2-sulfobutanoic acid in 15 DMA was added 130 mg (1.13 mmol) of NHS and 480 mg (2.50 mmol) of EDC. The mixture was stirred under Ar overnight, evaporated and purified on Si0 ₂ chromatography eluted with CH ₂ CH ₂ /CH ₃ OH/HCOOH (10000: 1000:1 to 10000: 1500:1) to afford 227 mg (82% yield) of the title compound. 1H NMR (DMSO-d6) 9.25 (d, 1H, J = 5.2 Hz), 8.57 (dd, 1H, J = 2.5, 8.9 Hz), 8.04 (t, 1H, J = 8.0 + 8.9 Hz), 3.86 (dd, 1H, J = 4.9, 9.7 Hz), 3.13 ~ 3.12 (m, 2H), 2.76 (s, 4H), 2.36 -2.30 (m, l H), 2.25 ~ 2.21 (m, 1H); 13C NMR 166.96, 165.01, 144.93, 142.26, 132.63, 1 19.61, 61.00, 35.03, 29.30, 25.39; MS m/z- 449.8 (M-H).

Methyl 2-(acetylthio)-4-bromobutanoate

[197] 10.0 g (38.4 mmol) of methyl 2,4-dibromobutanoate in 100 ml of dry THF at -

20°C was added dropwise the mixture of 2.75 ml (38.5 mmol) of thiolacetic acid in 8.5 ml (48.9 mmol) of DIPEA and 50 ml of dry THF in 1.5 hour. After stirring overnight at -20°C then 0°C for 2 hours under Ar, the mixture was concentrated, diluted with EtAc/Hexane, washed with 1.0 M NaH ₂ P0 ₄ , dried over MgS0 ₄ , filtered, evaporated, and Si02 chromatographic purification (1 : 12 to 1 : 10 EtAc/Hexane) to afford 9.5 g (96%) of the title compound. 1H NMR (CDC13) 4.38 (1H, t, J = 7.1Hz), 3.74 (s, 3H), 3.40 (m, 2H), 2.57 ~ 2.47 (m, 1H), 2.37 (s, 3H), 2.36 ~ 2.21 (m, 1H); 13C NMR 193.24, 171.36, 53.15, 44.45, 34.67, 30.46, 29.46; MS m/z+ 276.9 (M+Na), 278.9 (M+2+Na)

4-Bromo- 1 -methoxy- 1 -oxobutane-2-sulfonic acid

[198] 9.2 g (36.3 mmol) of methyl 2-(acetylthio)-4-bromobutanoate in 80 ml of acetic acid was added 40 ml of hydrogen peroxide (35% in water). The mixture was stirred overnight, then evaporated, diluted with water, neutralized with NaHC0 ₃ , washed with 1 : 1 Hexane/EtAc. The aqueous solution was evaporated, dissolved in methanol, concentrated, and crystallized with methanol/toluene to afford 8.6 g (90% yield) of the title compound, m.p. - 288- 293 (decomp); 1H NMR (D20) 4.12 (dd, 1H, J - 4.8, 9.3 Hz), 3.83 (s, 3H), 3.64 (m, 1H), 3.53 (m, 1H), 2.54 (m, 2H); 13C NMR 172.16, 66.73, 55.66, 33.39, 32.70; MS m/z- 260.8 (M-l). -(Acetylthio)- 1 -methoxy- 1 -oxobutane-2-sulfonic acid

[199] 5.0 g (19.2 mmol) of 4-bromo-l -methoxy- l-oxobutane-2-sulfonic acid in

100 ml of THF was added 3.0 ml of thioacetic acid and 9.0 ml of DIPEA in 100 ml of THF. The mixture was stirred overnight then refluxed at 70°C for 1 hr, evaporated and co- evaporated with 3 x 100 ml of water after neutralized to pH 7 with NaHC0 ₃ . The mixture was redissolved in methanol, filtered through celite, concentrated and purified with Si0 ₂ chromatography eluted with CH ₃ OH/CH ₂ Cl ₂ /HCOOH 37.5:250: 1 to 50:250:1) to afford 4.4 g (90% yield) of the title compound. 1H NMR(D20) 3.95 (dd, 1H, J = 4.1 , 10.3 Hz), 3.83 (s, 3H), 3.74 (m, 2H), 3.22 (dd, 2H, J = 7.4, 14.9 Hz), 2.39 (s, 3H); 13C NMR 203.88, 172.91, 67.32, 56.17, 29.04, 20.61 ; MS m/z- 254.8 (M-H) 4-((5-nitropyridin-2-yl)disulfanyl)-2-sulfobutanoic acid

[200] 3.0 g (1 1.7 mmol) of 4-(Acetylthio)-l-methoxy-l-oxobutane-2-sulfonic acid in 100 ml of water was added 50 ml of 3 M NaOH. After stirring under Ar for 3 h, the mixture was neutralized with 1 M H ₂ P0 ₄ to pH 7.2 under Ar. The mixture was added dropwise to the solution of 10.0 g (32.2 mmol) of l,2-bis(5-nitropyridin-2-yl)disulfane in 200 ml of DMA. After stirring for 4 h under Ar, the mixture was concentrated, diluted with water, filtered, evaporated and purified with C-18 4.0 x 20 cm column eluted with water/methanol (95:5) to afford 3.1 g (75% yield) of the title compound, m.p. = 288 ~ 291°C (decomp.) 1H NMR (DMF-d7) 9.29 (d, 1H, J = 2.2 Hz), 8.63 (dd, 1H, J = 2.7, 8.9 Hz), 8.17 (d, 1H, J = 8.9 Hz), 3.73 (t, 1H, J = 7.2 Hz), 3.22 ~ 3.17 (m, 1H), 3.15 ~ 3.10 (m, 1H), 2.41 ~ 2.33 (m, 2H); 13C NMR 170.92, 169.10, 146.04, 143.67, 133.65, 120.72, 64.22, 37.82, 29.26; MS m/z- 352.8 (M-H).

1 -(2,5-dioxopyrrolidin- 1 -yloxy)-4-((5-nitropyridin-2-yl)disulfanyl)-l -oxobutane-2-sulfonic acid

[201] 220 mg (0.62 mmol) of 4-((5-nitropyridin-2-yl)disulfanyl)-2-sulfobutanoic acid in 15 DMA was added 130 mg (1.13 mmol) of NHS and 480 mg (2.50 mmol) of EDC. The mixture was stirred under Ar overnight, evaporated and purified on Si0 ₂ chromatography eluted with CH ₂ CH ₂ /CH ₃ OH/HCOOH (10000: 1000:1 to 10000:1500: 1) to afford 227 mg (82% yield) of the title compound. 1H NMR (DMSO-d6) 9.25 (d, 1H, J = 5.2 Hz), 8.57 (dd, 1H, J = 2.5, 8.9 Hz), 8.04 (t, 1H, J = 8.0 + 8.9 Hz), 3.86 (dd, 1H, J = 4.9, 9.7 Hz), 3.13 ~ 3.12 (m, 2H), 2.76 (s, 4H), 2.36 -2.30 (m, 1H), 2.25 ~ 2.21 (m, 1H); 13C NMR 166.96, 165.01, 144.93, 142.26, 132.63, 119.61, 61.00, 35.03, 29.30, 25.39; MS m/z- 449.8 (M-H). 4-(pyridin~2-yidisulfanyl)-2-sulfobutanoic acid

Ϊ1 02] 1.5 g (5.85 mmol) of 4-(Acetylihio)- 1 -methoxy- 1 -oxobutane-2-sulfonic acid was added to 100 ml of 0.5 M NaOH solution. After stirring under Ar for 3 h, the mixture was concentrated to ~ 50 nil and neutralized with 1 M H ₂ PO4 to pH 7.2 under Ar. The mixture was added dropwise to the solution of 4.0 g (18.1 mmoi) of 2,2'-dithiodipyridme in 60 ml of DM A. After stirring for 4 h under Ar, the mixture was concentrated, diluted with water, filtered, evaporated and purified with C-18 4.0 x 20 cm column eluted with water/methanol (99: 1 to 90: 10) to afford 1.32 g (73% yield) of the title compound. 1H NMR (DMF-d7) 8.39 (eld, 1H, J = 3.5, 4.8 Hz), 7.86 (m, 2H), 7.25 (m, 1H), 3.59 (dd, 1 H, J = 5.2, 9.4 Hz), 2.90 (m. 2H), 2.28 (m, 2H); I 3C NMR 172.60, 159.16, 148.93, 138.09, 121.03, 1 19.38, 67.49, 36.39, 28.666; MS mJz- 307.8 (M-H). -3ulfonic acid

[203] 680 mg (2.20 mmol) of 4-(pyridin-2-yldisulfanyl)-2-suifbbutanoic acid in 50

DMA was added 300 mg (2.60 mmol) of NHS and 800 mg (4.16 mmol) of EDC. The mixture was stirred under Ar overnight, evaporated and purified on S1O ₂ chromatography eluted with€Η ₂ ΟΗ ₂ /α¾ΟΗ/Η(Χ)ΟΗ (10000: 1000: 1 to 10000:1500: 1) to afford 720 mg (80% yield) of the title compound. 1H NMR (DMSOd6) 8.40 (dd, 1 H, J - 3.5, 4.7 Hz), 7.85 (m, 2H), 7.24 (m, 1H), 3.58 (dd, 1H, J = 5.1, 9.4 Hz), 2.94 ~ 2.90 (m, 2H), 2.74 (s, 4H), 2.31 -2.27 (m, 21 1); I 3C NMR 168.16, 161 ,11, 147.91 , 139.22, 121.63, 1 19.31 , 66.80, 36.30, 28.36, 25.42: MS m/z- 404.9 (M-H).

3 , 6-endoxo-A-tetrah droplrthalhide [204] Maleimide (5.0 g, 51.5 mmol) in ethylether (200 ml) was added furan (5.5 ml,

75.6 mmol). The mixture was heated inside a 1 L of autoclave bomb at 100°C for 8 h. The bomb was cooled down to room temperature, and the inside solid was rinsed with methanol, concentrated and crystallized in ethyl acetate/hexane to afford 8.4 g (99%) of the title compound. 1H NMR (DMF-d7): 1 1.08 (s, 1H) (NH), 6.60 (m, 2H), 5.16 (m, 2H), 2.95 (m, 2H). 13C NMR 178.84, 137.69, 82.00, 49.92. MS m/z+ 188.4 (MW + Na).

Methyl 4-N- 3, 6-endoxo-A-tetrahydrophthalido)-2-sulfo-butyrate

[205] 3, 6-Endoxo-A-tetrahydrophthalhide (0.80 g, 4.85 mmol) in DMA (20 ml) was added K ₂ C0 ₃ (1.4 g, 10.13 mmol) and KI (0.19 g, 1.14 mmol). After stirring under Ar for 1 hr, methyl 4-bromo-2-sulfo-butyrate (0.98 g, 3.77 mmol) in DMA (10 ml) was added. The mixture was stirred under Ar overnight, evaporated, re-dissolved in 1 % HAc in methanol, filtered, evaporated and purified by Si0 ₂ chromatography and eluted with 1 :5:0.01 to 1 :4:0.01 CH ₃ OH/CH ₂ Cl ₂ /HAc to afford 0.98 (75%) g of the title compound. 1H NMR (DMF- d7): 6.59 (m, 2H), 5.16 (dd, 2H, J = 0.8, 7.8 Hz), 3.65-3.63 (m, 3H), 3.47 (m, 2H), 3.01 (s, 3H), 2.83 (m, 2H). 13C NMR 172.94, 162.86, 137.68, 81.98, 52.39, 49.91, 48.58, 36.01 , 21.97. MS m/z- 343.9 (MW - H).

Methyl 4-N-maleimido2-sulfo-butyrate

[206] In an opened round bottom flask, methyl 4-N-(3, 6-endoxo-A- tetrahydrophthalido)-2-sulfo-butyrate (0.30 g, 0.87 mmol) in 20 ml of 1 :1 DMA/ 100 mM NaH ₂ P0 ₄ , pH 7.0 was heated at 120 ~ 140°C for 4 h. During the reaction time, 5 x 10 ml of water was gradually added to keep the reaction volume around 15 ml. The mixture was concentrated to dryness and purified by Si0 ₂ chromatography eluted with 1 :5:0.01 to 1 :4:0.01 CH ₃ OH/CH ₂ Cl ₂ /HAc to afford 0.230 g (95%) of the title compound. Ή NMR (DMF-d7): 6.60 (s, 2H), 4.06 (d, 1H), 3.60 (m, 3H), 3.47 (m, 2H), 2.43 (m, 2H); ^li C NMR 171.59, 164.96, 136.10, 66.20, 51.71, 34.82, 22.10. MS m/z- 276.6 (MW - H).

Methyl 4-azido-2-sulfo-butyrate

[207] Methyl 4-bromo-2-sulfo-butyrate (1.07 g, 4.1 1 mmol) and sodium azide (0.70 g (10.7 mmol) in DMF (50 ml) was stirred overnight. The mixture was evaporated and purified by Si02 chromatography and eluted with 1 :5:0.01 CH30H/CH2C12/HAc and crystallized with CH30H/Toluene/Hexane to afford 1.00 g (95%) of the title compound, m.p = 267 -272 oC (decomp). 1H NMR (DMF-d7): 12.06 (br, 1H), 3.65 (s, 3H), 3.59 (dd, 1H, J = 5.4, 8.9 Hz), 3.47 (m, 2H), 2.24 (m, 2H). 13C NMR 171.10, 64.29, 52.24, 50.64, 21.35. ESI MS m/z+ 267.9 (M + 2Na-H), m/z- 222.0 (M-H). HRMS m/z- (C5H9N305S -H) calcd 222.0185, found 222.0179.

4-azido-2-sulfo-butyric acid

[208] Methyl 4-azido-2-sulfo-butyrate (1.00 g, 4.08 mmol) in the mixture of HC1 (50 ml, 1.0 M) and HAC (5 ml) was heated at 100°C for 8 hrs. The mixture was evaporated and co-evaporated 3x 50 ml of water, and crystallized with water/acetone to afford 1.0 g (99%) of the title compound. 1H NMR (DMF-d ₇ ): 3.60 (m, 2H), 3.52 (m, 1H), 2.24 (m, 2H). ^I3 C NMR 170.96, 63.04, 50.66, 29.12. ESI MS m/z- 207.7 (MW -H); HRMS m/z- (C ₄ H ₇ N ₃ 0 ₅ S - H) calcd 208.0028, found 208.0021.

4-Amino-2-sulfo-butyric acid [209] 4-Azido-2-sulfo-butyric acid (500 mg, 2.40 mmol), water (20 ml) and Pd/C

(1 10 mg, 10% Pd, 50%) water based) were placed into a 250 ml hydrogenation shaking bottle. After the air in the bottle was sucked out by a vacuum, 20 psi of hydrogen was let into the bottle. The mixture was shaken for 8 h, then filtered through celite, washed with DMF, evaporated and co-evaporated with dry DMF to afford 476 mg (91% HCl salt) of the title product. ESI MS m/z- 181.8 (MW -H). This product was used directly without further purification.

(Z)-4-(3-carboxy"3-sulfopropyiamino)-4-oxobut~2-enoic acid

[210] The above 4-Amino-2-sulfo-butyric acid, HCl salt (476 mg, 2.16 mmol) in dry

DMF (20 ml) was added maleic anhydride (232 mg, 2.36 mmol). The mixture was stirred under Ar overnight, evaporated and purified on self packed c-18, φΐ .0 x 25 cm column, eluted with water. The fractions contained product were pooled, evaporated and crystallized with H ₂ 0/acetone to afford 552 mg (91%) of the title product. Ή NMR (DMF-d7): 9.70 (br, 1H), 6.73 (d, 1H, J = 12.8 Hz), 6.32 (d, 1H, J = 12.8 Hz), 3.69 (m, 1H), 3.47 (m, 2H), 2.27 (m, 2H). ¹³ C NMR 171.47, 167.32, 165.87, 135.44, 133.07, 63.82, 39.13, 27.62. ESI MS m/z- 279.8 (MW -H); HRMS m/z- (C ₈ H _n N0 ₈ S -H) calcd 280.0127, found 280.0121.

4-N-Maleimido-2-sulfo-butanoic acid

[211] (Z)-4-(3-carboxy-3-sulfopropylamino)-4-oxobut-2-enoic acid (310 mg, 1.10 mmol) in mixture dry DMA (5 ml) and dry toluene (20 ml) was heated. After the temperature reached at 80°C, HMDS (hexamethyldisilazane) (1.40 ml, 6.71 mmol) and ZnCl ₂ (1.85 ml, 1.0 M in diethyl ether, 1 .85 mmol) was added. The mixture was continued heated to 1 15 ~ 125°C and toluene was collected through Dean-Stark trap. The reaction mixture was fluxed at 120 °C for 6 h. During this period, 2 x 20 ml of dry toluene was added to keep the mixture volume around 8 - 10 ml. Then the mixture was cooled, 1 ml of 1 : 10 HC1 (conc)/CH ₃ OH was added, evaporated, purified on Si0 ₂ chromatography eluted with CH ₃ OH/CH ₂ Cl ₂ /HAc (1 :5:0.01 to 1 :4:0.01) to afford 260mg (92%) of the title product. Ή NMR (DMF-d ₇ ): 10.83(br, 1H), 6.95 (s, 2H) , 1H, J = 12.8 Hz), 3.65 (m, 1H), 3.54 (m, 2H), 2.27 (m, 2H). ¹³ C NMR 173.61 , 172.04, 135.47, 64.18, 37.1, 27.89. ESI MS m/z- 261.8 (MW -H). HRMS m/z- (C ₈ H ₉ N0 ₇ S -H) calcd 262.0021, found 262.0027.

Succini idyl 4-N-maleimido-2-sulfo-butyrate

[212] 4-N-maleimido-2-sulfo-butanoic acid (260 mg, 0.99 mmol) in DMA (10 ml) was added to NHS (220 mg, 1.91 mmol) and EDC (500 mg, 2.60 mmol). The mixture was stirred under Ar overnight, evaporated and purified on Si0 ₂ chromatography eluted with CH ₂ CH ₂ /CH ₃ OH/HAc (10000: 1000: 1 to 10000:2000: 1), then crystallized with DMA/EtAc/Hexane to afford 285 mg (81% yield) of the title compound. Ή NMR (DMF-d7) 6.99 (s, 1H), 3.83 (m, 1H), 3.64 (m, 2H), 2.75 (s, 4H), 2.34 (m, 2H); ¹³ C NMR 171.97, 171.82, 166.64, 135.58, 62.00, 36.66, 26.62; ESI MS m/z- 358.9 (M-H); HRMS m/z- (Ci ₂ Hi ₂ N ₂ 0 ₉ S -H) calcd 359.0185, found 359.0178.

(E)-Methyl 4-azidobut-2-enoate

[213] To the solution of NaN ₃ (2.80 g, 43.01 mmol) in 100 ml of DMF at -20°C was added methyl 4-bromocrotonate (5.00 ml, 85%, 36.10 mmol). After stirred at -20°C for 30 min, the mixture was stirred at 0°C for 4 h, evaporated, suspended with EtAc/Hexane (1 : 1), filtered, evaporated and chromatographic purification on Si0 ₂ column eluted with EtAc/Hexane (1 :25 to 1 : 10 ) to afford HRMS for 4.08 g (80%) of the title product. ^! H NMR (CDC1 ₃ ) 6.88 (m, 1H), 6.06 (ddd, 1H, J -= 1.7, 3.4, 15.6 Hz), 3.97 (dd, 2H, J = 1.2, 4.96 Hz), 3.73 (s, 3H); ¹³ C NMR 166.23, 140.86, 123.49, 51.95, 51.36; ESI MS m/z+ 182.5 (M+ Na + H ₂ 0); HRMS m/z+ (C ₅ H ₇ N ₃ 0 ₂ + H ₂ 0 + Na) calcd 182.0542, found 182.0548.

Methyl 3-(acetylthio)-4-azidobutanoate

[214] To the solution of (E)-Methyl 4-azidobut-2-enoate (4.00g, 28.37 mmol) in 60 ml of THF at 0"C was added the mixture of thiolacetic acid (3.0 ml, 42.09 mmol) and DIPEA (8.0 ml, 45.92 mmol) in 60 ml of THF in 20 min. After stirred at 0°C for 1 hr, the mixture was stirred at RT overnight, evaporated, redissolved in CH ₂ C1 ₂ , washed with NaHC0 ₃ (sat.) and 1 M NaH ₂ P0 ₄ /NaCl (sat.), pH 4 respectively, dried over MgS04, filtered, evaporated and chromatographic purification on Si0 ₂ column eluted with EtAc/Hexane (1 :8 to 1 : 4) to afford HRMS for 4.98 g (81%) of the title product. Ή NMR (CDC1 ₃ ) 3.66 (m, 1H), 3.62 (s, 3H), 3.40 (dd, 1H, J = 7.5, 12.7 Hz), 3.31 (m, lH), 2.78 (m, 1H), 2.60 (m, 1H), 2.32 (s, 3H); ¹³ C NMR (DMF-d7) 192.20, 172.48, 56.56, 53.60, 51.31, 34.58, 30.56; ESI MS m/z+ 240.0 (M+ Na), 255.9 (M+ K); HRMS m/z+ (C ₇ H _n N ₃ 0 ₃ S+ Na) calcd 240.0419, found 240.0415.

Azido-4-methoxy-4-oxobutane-2-sulfonic acid

[215] Methyl 3-(acetylthio)-4-azidobutanoate (4.00 g, 18.43 mmol) in 75 ml of acetic acid was added 25 ml of H ₂ 0 ₂ (30%). The mixture was stirred overnight, evaporated and co-evaporated with EtOH/toluene and purified on Si0 ₂ chromatography eluted with CH ₃ OH/CH ₂ Cl ₂ /HAc (100:800: 1 to 100:500: 1) to afford 3.85 (93%) g the title compound. ^] H NMR (CD ₃ OD) 3.78 (dd, 1H, J = 5.0, 12.7 Hz), 3.62 (s, 3H), 3.44 (dd, 1H, J = 7.5, 12.7 Hz), 3.33 (m, 1H), 2.84 (dd, 1H, J = 5.6, 16.5 Hz), 2.57 (dd, 1H, J = 7.5, 16.5 Hz); ¹³ C NMR (DMF-d7) 173.37, 57.31, 52.54, 52.49, 34.51 ; ESI MS m/z- 221.7 (M+ H),

4-Azido- -sulfobutanoic acid

[216] Azido-4-methoxy-4-oxobutane-2-sulfonic acid (3.80 g, 17.04 mmol) in 150 ml of 1.0 M HC1 was added 8.0 ml of HAc. The mixture was refluxed at 120°C overnight, evaporated and co-evaporated with water, EtOH, EtOH/toluene respectively and purified on Si0 ₂ chromatography eluted with CH ₃ OH/CH ₂ Cl ₂ /HAc (100:500: 1 to 100:400: 1) to afford 3.02 (85%) g the title compound. Ή NMR (CD ₃ OD) 3.77 (dd, 1H, J = 5.1, 12.8 Hz), 3.45 (dd, 1H, J = 7.0, 12.8 Hz), 3.31 (m, 1H), 2.86 (dd, 1H, J = 4.7, 16.7 Hz), 2.51 (dd, 1H, J = 8.4, 16.7 Hz); ¹³ C NMR (DMF-d7) 173.98, 67.50, 59.78, 27.82; ESI MS m/z- 207.7 (M -H).

4-amino-3-sulfobutanoic acid

[217] In a 500 ml of hydrogenation bottle was added 4-azido-3-sulfobutanoic acid

(3.00 g, 14.35 mmol), 150 ml of methanol and 0.32 g of Pd/C (10% Pd, 50% wet). After sucked out air, 30 psi of H2 was conducted, and the mixture was shaken overnight, filtered through celite, evaporated, and coevaporated with dry EtOH to afford about 2.50 g (95%) of 4-amino-3-sulfobutanoic acid. Ή NMR (CD ₃ OD) 3.24 (m, 1H), 3.17 (m, 1H), 2.90 (dd, 1H, J = 2.6, 16.5 Hz), 2.33 (dd, 1H, J = 10.1, 16.5 Hz), ESI MS m/z- 181.60 (M-H). The resulted compound was unstable and was used directly without further purification.

(Z)-4-(3- rboxy-2-sulfopropylamino)-4-oxobut-2-enoic acid

[218] To the solution of 4-amino-3-sulfobutanoic acid (~ 2.50 g, 13.66 mmol) in 100 ml of DMA was added maleic anhydride (1.48 g, 15.10 mmol) and the mixture was stirred over night, evaporated, purified on C-18 column (2 x 30 cm) eluted with 1% HAc in water and crystallized with MeOH/Acetone/toluene to afford 3.34 g (83%) of (Z)-4-(3-carboxy-2- sulfopropylamino)-4-oxobut-2-enoic acid. Ή NMR (CD ₃ OD) 6.33 (d, 1H, J = 12.6 Hz), 6.10 (d, 1H, J = 12.6 Hz), 3.64 (dd, 1H, J = 5.8, 14.0 Hz), 3.54 (m, 1H), 3.30 (m, 1H), 2.78 (dd, 1H, J = 4.9, 16.8 Hz), 2.39 (m, 1H); ¹³ C NMR 173.52, 168.68, 167.98, 135.59, 127.79, 57.31, 40.56, 34.52; ESI MS m/z- 279.7 (M -H). 4-(2,5-dioxo-2,5-dihydro- 1 H-pyrrol- 1 -yl)-3 -sulfobutanoic acid

[219] (Z)-4-(3-carboxy-2-sulfopropylamino)-4-oxobut-2-enoic acid (450 mg, 1.60 mmol) in mixture of 10 ml of dry DMA and 50 ml of dry toluene was heated. After the temperature reached at 80°C, HMDS (hexamethyldisilazane, 1.80 ml, 8.63 mmol, ) and ZnCl ₂ (3.2 ml, 1.0 M in diethyl ether) were added. The mixture was continued heated to 1 15 ~ 125 °C and toluene was collected through Dean-Stark trap. The reaction mixture was fluxed at 120 °C for 6 h. During this period, 2 x 20 ml of dry toluene was added to keep the mixture volume around 8 ~ 10 ml. Then the mixture was cooled, 1 ml of 1 : 10 HC1 (conc)/CH ₃ OH was added, evaporated, purified on Si0 ₂ chromatography eluted with 1 :5:0.01 C¾OH/CH ₂ Cl ₂ /HAc to afford 315 mg (75%) of the title product. 1H NM (DMF-d7) 6.96 (s, 2H), 4.04 (dd, 1H, J = 4.3, 13.8 Hz), 3.47 (m, 1H), 3.23 (dd, 1H, J = 7.4, 14.7Hz), 2.99 (dd, 1H, J = 3.3 , 16.8 Hz), 2.35 (dd, 1H, J = 8.1, 16.9 Hz); ¹³ C NMR 173.58, 172.18, 135.54, 54.61, 40.24, 32.43, ESI MS m/z- 261.70 (M -H).

1 -(2,5-Dioxo-2,5-dihydro- 1 H-pyrrol- 1 -yl)-4-(2,5-dioxopyrrolidin- 1 -yloxy)-4-oxobutane-2- sulfonic acid

[220] 4-(2,5-Dioxo-2,5-dihydro-l H-pyrrol- l-yl)-3-sulfobutanoic acid (1 10 mg,

0.418 mmol), EDC (240 mg, 1.25 mmol) and N-hydroxysuccinimide (58 mg, 0.504 mmol) was stirred in 10 ml of DMA for overnight, evaporated and purified on Si0 ₂ chromatography eluted with CH ₃ OH/CH ₂ Cl ₂ /HAc (100:900: 1 to 100: 600: 1) to afford 1 12 mg (75%) of the title product. Ή NMR (DMF-d7) 6.93 (s, 2H), 4.06 (dd, 1H, J = 4.8, 13.1 Hz), 3.80 (dd, 1H, J - 10.7, 13.9 Hz), 3.35 (dd, 1H J = 3.3, 17.8 Hz), 3.25 (m, 1H), 3.10 (dd, 1H, J = 2.2, 16.4 Hz), 2.87 (m, 4H); ¹³ C NMR 172.27, 170.88, 169.29, 135.55, 55.28, 40.22, 32.69, 26.66; ESI MS m/z- 261.70 (M -H). Ethyl 3-(acetylthio)-3-cyanopropanoate

i ^N ft HSAc/Et3N ? ^N ¾

⁰ THF, 0*C, 65% ^Ac¾ °

[221] (Z)-ethyl 3-cyanoacrylate (5.01 g, 40.00 mmol) in 80 ml of THF at -20°C was added the solution of thiol acetic acid (5.0 ml, 70.15 mmol) and DIPEA (16.0 ml, 92.03 mmol) in 20 ml of THF in 30 min. The reaction was kept at -20°C for 4 hr then room temperature overnight. The mixture was concentrated, diluted with CH ₂ C1 ₂ , washed with saturated NaHC0 ₃ , dried over MgS0 ₄ , filtered, evaporated and purified by Si0 ₂ chromatography (1 :4 EtAC/Hexane) to afford 5.22 g (65%) of the title compound. Rf =0.25 (1 :4 EtAC/Hexane); 1H NMR (CDC1 ₃ ), 4.44 (m, 1H), 4.1 1 (dd, 2H, J = 7.1, 14.3 Hz), 3.38 (m, 1H), 3.15 (m, 1H), 2.17 (s, 3H), 1.19 (t, 3H, J = 7.2 Hz); ¹³ C NMR 194.12, 173.21, 1 19.82, 61.35, 33.52, 30.08, 14.62; MS m/z+ 225.9 (MW + Na), m/z- 201.7 (MW-H).

Cyano-3 -ethoxy-3 -oxopropane- 1 -sulfonic acid

[222] Ethyl 3-(acetylthio)-3-cyanopropanoate (2.00g, 9.95 mmol) in acetic acid (40 ml) was added H ₂ 0 ₂ (12 ml, 30%). The mixture was stirred overnight, evaporated and purified on silica gel chromatography eluted with methanol/dichloromethane/acetic acid (1 :8:0.01 to 1 :5:0.01) to afford 1.72 g (84%) of the title compound. Ή NMR (DMSO), 4.63 (m, 1H), 4.12 (dd, 2H, J = 7.1, 14.3 Hz), 3.27 (m, 1H), 3.05 (m, 1H), 1.28 (t, 3H, J = 7.2 Hz); ¹³ C NMR 173.15, 1 13.85, 61.38, 48.32, 26.33, 14.15; MS m/z- 205.7 (MW-H). l-(tert-Butoxycarbonylamino)-4-ethoxy-4-oxobutane-2-sulfonic acid

[223] In a hydrogenation bottle was added Cyano-3 -ethoxy-3 -oxopropane- 1 -sulfonic acid (2.50 g, 12.06 mmol), ethanol (80 ml), fresh filtered Raney Nickel (0.40 g) and BOC anhydride (3.30 g, 15.12 mmol). After the air inside the bottle was sucked out by vacuum, 20 psi of hydrogen was conducted to the bottle. The bottle was shaken over night, filtered through celite, evaporated, and purified on silica gel chromatography eluted with methanol/dichloromethane/acetic acid (1 :6:0.01) to afford 3.18 g (85%) of the title compound. Ή NMR (DMSO), 6.82 (s, IH), 4.26 (m, IH), 4.1 1 (dd, 2H, J = 7.1, 14.3 Hz), 3.53 (dd, IH, J = 4.2, 13.4 Hz), 3.36 (m, IH), 2.86 (m, IH), 2.51 (m, IH), 1.38 (s, 9H), 1.22 (t, 3H, J = 7.2 Hz); ¹³ C NMR 173.35, 155.72, 80.44, 62.05, 52.55, 41.61, 34.50, 28.85, 14.52; MS m/z- 309.8 (MW-H).

4-(tert-butoxycarbonylamino)-3-sulfobutanoic acid

[224] l-(tert-Butoxycarbonylamino)-4-ethoxy-4-oxobutane-2-sulfonic acid (402 mg,

1.29 mmol) in the mixture of THF/H ₂ 0 (1 :2, 60 ml) was added lithium hydroxide monohydrate (2.0 g, 47.6 mmol). The mixture was stirred under Ar overnight, concentrated, purified on C-18 column (2 x 30 cm) eluted with from 100% water to 10% methanol in water to afford 328 mg (90%) of the title compound. 1H NMR (DMSO), 6.78 (s, IH), 4.03 (m, IH), 3.57 (dd, IH, J = 4.2, 13.4 Hz), 3.41 (m, IH), 2.89 (m, IH), 2.61 (m, IH), 1.39 (s, 9H); ¹³ C NMR 174.21 , 155.82, 79.85, 59.95, 42.06, 32.52, 28.88, 14.55; ESI MS 281.8 (M-H);

(Z)-4-(3-carboxy-2-sulfopropylamino)-4-oxobut-2-enoic acid

[225] 4-(Tert-butoxycarbonylamino)-3-sulfobutanoic acid (321 mg, 1.13 mmol) was stirred in the mixture of HC1 (conc)/Dioxane (1 :4, 15 ml) for 30 min, evaporated and coevaporated with EtOH/Toluene (1 : 1, 4 x 20 ml) to dryness. To the dryness material was added maleic anhydride (121 mg, 1.23 mmol) and DMA (20 ml) and the mixture was stirred overnight, evaporated and run tlirough C-18 column eluted with water and crystallized with EtOH/Hexane to afford 263 mg (83%) of the title compound. ESI MS 279.8 (M- H). The NMR data are the same through the route with 4-azido-3-sulfobutanoic acid. N,N,N-trimethyl-2-oxotetrahydrothiophen-3-aminium

[226] 3-aminodihydrothiophen-2(3H)-one hydrochloride (6.00 g, 39.1 mmol), sodium bicarbonate (3.28 g, 39.1 mmol) and iodomethane (13 mL, 209 mmol) were stirred in dry methanol (100 ml) overnight, filtered through celite, evaporated, purified on Si02 column eluted with MeOH/CH ₂ Cl ₂ /HAc (1 :5:0.01), and crystallized with EtOH/Hexane to afford 5.25 g (84%) of the title product, mp 228- 231°C. 1H NMR (CD ₃ OD) 4.27 (m, 1H), 3.25 (s, 9H), 2.56 - 2.47 (m, 2H), 2.34 (m, 1H), 2.26 (m, 1H); ¹³ C NMR 168.97, 75.06, 53.25, 30.85, 16.46; ESI MS m/z+ 160.0 (M+).

1 -carboxy-N,N,N-trimethyl-3-(pyridin-2-yldisulfanyl)propan- 1 -aminium

[227] N,N,N-trimethyl-2-oxotetrahydrothiophen-3-aminium acetate (2 g, 9.13 mmol) was stirred in 75 ml of 1 M NaOH (3 g NaOH in 75 ml H ₂ 0) for 45 min. neutralized with 4 M H ₃ P0 ₄ to pH 7.4, concentrated, added to l,2-di(pyridin-2-yl)disulfane (11 g, 49.9 mmol) in 200 ml of MeOH. The mixture was stirred over night, extracted with EtAc. The aqueous solution was evaporated, suspended with MeOH, filtered salt, evaporated and purified on C-18 column (2 cm x 30 cm) eluted with water/methanol (100 water to 20% methanol/water) to afford 2.6 g (75%) of the title product. ESI MS m/z+ 309.1 (M +Na-H).

1. Modification of antibody with sulfo linker

[228] The huC242 is modified with sulfo linker at 8 mg/mL antibody, a 15 fold molar excess of sulfo linker (~30mM stock solution in DMA). The reaction is carried out in 100 mM NaPi, pH8.0 buffer with DMA (5% v/v) for 15, 30, 120, and 200 minutes at 25 °C. The modified huC242 was purified by G25 column with 50 mM NaPi, 50 mM NaCl, and 2 mM EDTA, pH6.5 to remove the excess sulfo linker. 2. Measurement of releasable Spy-N0 ₂ and antibody concentration of modified huC242

[229] The assay and spectral measurement were carried in 100 mM NaPi, pH7.5 at room temperature. The molar ratio of Spy-N0 ₂ released per mole of huC242 antibody was calculated by measuring the A ₂₈ o of the sample and then the increase in the A ₃₉₄ of the sample after adding DTT (50 μΐ, of 1 M DTT/mL of sample). The concentration of DTT-released 2- mercaptopyridine is calculated using a ε ₃₉ 4 _nm of 14,205 M^cm ^"1 . The concentration of antibody can then be calculated using a ε _{280 nm} of 217,560 M^cm ^"1 after subtracting the contribution of Spy-N0 ₂ absorbance at 280 nm (A _{394 nm} post DTT x 3344/14205) from the total A ₂₈ o _nm measured before DTT addition. The molar ratio of Spy-N0 ₂ :Ab can then be calculated. The mg/mL (g/L) concentration of huC242 is calculated using a molecular weight of 147,000 g/mole.

3. Conjugation reaction

[230] The modified huC242 was reacted with a 1.7-fold molar excess of DM4

(based on DM4 stock SH concentration) over Spy-N0 ₂ . The reaction is carried out at 2.5 mg/mL antibody in 50 mM NaPi, 50 mM NaCl, 2 mM EDTA, pH6.5 and DMA (5% v/v). After addition of DM4, the reaction was incubated 25°C for -20 hours. The final conjugate was purified by G25 column with 10 mM Histidine, 130 mM Glycine, 5% sucrose, pH5.5 to remove the excess DM4 drug.

4. Calculation of huC242 and DM4 concentration

[231] The huC242 and DM4 both absorb at the two wavelengths used to measure each component separately, i.e., 280 and 252 nm. The extinction coefficient at 280 nm for huC242 is 217,560 and for DM4 is 5180 M ^"1 . The 252 nm/280 nm absorbance ratios of huC242 and DM4 are 0.368 and 5.05 respectively. The concentrations were calculated with following equation

C _D = Α2^ - 0.368Α7 _Χ π C _Ab = A7gn - 5180Cn

24692.4 217,560 Results

C242-Sulfb-DM4 linker titration

Conj ugation protocol :

[232] Modification was done at pH 8.0, buffer A and 5% DMA for 90 min at room temperature, the antibody concentration is 7 mg/ml. The modificed antibody was purified by

NAP column using Buffer A pH6.5, The conjugation was down at Buffer A, pH6.5 with 5- 10% DMA at room temperature overnight. The drug to linker ratio ranged from 1.3 to 1.7 deepening on the total drug added. Example 2: Conjugate Synthesis.

[233] SPP or SSNPP linker was dissolved in ethanol at a concentration of approximately 10 mM. Antibody was dialyzed into buffer A (50 mM KPi, 50 mM NaCl, 2 mM EDTA, pH 6.5). For the linker reaction, the antibody was at 8 mg/ml, and 7 equivalents of linker were added while stirring in the presence of 5% (v/v) ethanol. The reaction was allowed to proceed at ambient temperature for 90 minutes. Unreacted linker was removed from the antibody by Sephadex G25 gel filtration using a Sephadex G25 column equilibrated with Buffer A at pH 6.5 or 150 mM potassium phosphate buffer containing 100 mM NaCl, pH 7.4 as indicated. For the SPP linker, the extent of modification was assessed by release of pyridine-2-thione using 50 mM DTT and measuring the absorbance at 343 nm as described below (ε343 = 8080 M ^"1 cm ^"1 for free pyridine-2-thione). For SSNPP, modification was assessed directly by measuring the absorbance at 325 nm (ε ₃₂₅ = 10,964 M ^"1 cm ^"1 for the 4- nitropyridyl-2-dithio group linked to antibody). For the conjugation reaction, thiol- containing drug (either DM1 or DC4) was dissolved in DMA (N, N-dimefhylacetamide) at a concentration of approximately 10 mM. The drug (0.8 - 1.7-fold molar excess relative to the number of linker molecules per antibody as indicated) was slowly added with stirring to the antibody which was at a concentration of 2.5 mg/ml in buffer A (pH 6.5 or pH 7.4) in a final concentration of 3% (v/v) DMA. The reaction was allowed to proceed at ambient temperature for the indicated times. Drug-conjugated antibody was purified using a Sephadex G25 column equilibrated with buffer B (PBS, pH 6.5). For DML, the extent of drug conjugation to antibody was assessed by measuring A ₂₅₂ and A ₂₈ o of the conjugate as described below. A similar approach was used for DC4 (see below).

Measurement of Releasable Pyridine-2-thione and Ab Concentration SPP- odi lied Ab.

[234] The molar ratio of pyridine-2-thione released per mole of antibody is calculated by measuring the A ₂ g ₀ of the sample and then the increase in the A ₃₄₃ of the sample after adding DTT (50 of 1 M DTT/mL of sample). The concentration of DTT-released pyridine-2-thione is calculated using an ε ₃₄₃ of 8080 Μ ^' Όη ^"1 . The concentration of antibody can then be calculated using an ε ₂₈₀ of 194,712 M^cm ^"1 after subtracting the contribution of pyridine-2-thione absorbance at 280 nm (A _{343 nm} post DTT x 5100/8080) from the total A ₂₈₀ nm measured before DTT addition. The molar ratio of pyridine-2-thione:Ab can then be calculated. The mg/mL (g/L) concentration of Ab is calculated using a molecular weight of 147,000 g/mole. Measurement of antibody- linked 5 -Nitrop ridyl-2-dithio Groups and Ab Concentration of SSNPP-Modified Ab.

[235] The molar ratio of the 4-nitropyridyl-2-dithio groups linked per mole of antibody is calculated by measuring the Α ₂₈ ο an A ₃₂₅ of the sample without DTT treatment. The number of antibody-bound 4-nitropyridyl-2-dithio groups is calculated using an ε _{325 nm} of 10,964 Μ ^' Όη ^"1 . The concentration of antibody can then be calculated using an ε ₂₈₀ nm of 194,712 M^cm ^"1 after subtracting the contribution of the 5-nitropyridyl-2-dithio group absorbance at 280 nm (A _{325 nm} x 3344/10964) from the total A _{280 n} m measured. The molar ratio of 4-nitropyridyl-2-dithio groups :Ab can then be calculated. The mg/mL (g/L) concentration of Ab is calculated using a molecular weight of 147,000 g/mole.

Calculating Ab and DM1 component concentrations of Ab-DMl .

[236] The Ab and DM1 both absorb at the two wavelengths used to measure each component separately, i.e., 280 and 252 nm. The components are quantified using the following algebraic expressions which account for the contribution of each component at each wavelength (C _Ab is the molar concentration of Ab and Co is the molar concentration of DM1):

1) Total A ₂₈₀ = 194,712C _A b + 5,700C _D

2) Total A ₂₅₂ =(194,712 x 0.37)C _Ab + (4.7 x 5,700) C _D

Each equation is solved for C _Ab :

194,712

2a) C _Ah = A _{z 2} - 26.790Cn

72,043

and an equality is set up (equation la = equation 2a) and solved for Co:

C _D = A?y - 0.37A _Z8 _n

24,681

[237] Once the Co is calculated, the value is used to solve for C _Ab in equation la (or

2a) above. The ratio of DM1 :Ab can then be calculated. The mg/mL (g/L) concentration of antibody is calculated using a molecular weight of 147,000 g/mole and the concentration of DM1 is calculated using a molecular weight of 736.5 g/mole (linked DM1) 1 ^■ fliciency o f disul fide exchange is increased with SSNPP.

[238] As shown in Table 1, the efficiency of conjugation is enhanced in reactions where SSNPP is used as the cross-linker compared to reactions using SPP. The percent efficiency was calculated by dividing the value for DM1 per antibody by the linker per antibody ratio times 100. Conjugations of the N901 antibody using SSNPP resulted in cross- linking efficiencies of 93% at both pH 6.5 and 7.4. The efficiency of conjugation of N901 with SPP in these experiments was 70% at pH 6.5 and 77% at pH 7.4. The increased efficiency with SSNPP demonstrates that a target DM1 to antibody ratio can be achieved using antibody that is modified with a reduced number of linker molecules. In fact, a similar drug to antibody ratio (4.3) was achieved in the final conjugate with an antibody preparation having 4.2 (5-nitropyridyl-2-dithio)-groups per antibody introduced with SSNPP compared to an antibody having 5.6 pyridyl-2-dithio groups introduced with SPP (Table 2). The amount of drug required to obtain comparable conjugation results was therefore 25% lower for the SSNPP-modified antibody than the SPP-modified antibody under these conditions. An additional potential benefit of the increased efficiency with SSNPP is that a reduced molar excess of DM1 may be used in the conjugation reaction. A comparison of the DM1 per antibody ratios following conjugation with a range of drug equivalents in the reaction (0.8 - 1.7 fold excess) shows that a 1.1 -fold molar excess is sufficient to achieve 100% conjugation efficiency using the SSNPP cross-linker (Figure 7). A comparison of the time course of the reaction of DM1 with antibody that had been modified with SSNPP or SPP is shown, for example, in Figure 8. In each case the modified antibody was treated with a 1.1 -fold molar excess of DM1 per mole of linker incorporated. The reaction with the SSNPP-modified antibody is considerably faster than with the SPP-modified antibody (Figure 8). Even, a molar excess of 1.7-fold is not sufficient to achieve a similar efficiency using SPP. The ability to use 1) a lower molar excess of DM1 and 2) fewer linkers per antibody allows a reduction in the amount of drug needed to achieve a target DM1 to antibody ratio by as much as 50% when using SSNPP as the cross-linker instead of SPP.

[239] The increased efficiency of conjugation using the SSNPP linker is

accomplished without compromise in the monomeric character of the conjugate and in the amount of unconjugated (free) drug associated with the antibody conjugate. SEC analysis is used to determine the amount of monomer, dimer, trimer, or higher molecular weight aggregates. Typical results of greater than 90% monomer were obtained with either linker as shown in Table 1. The level of unconjugated drug was measured by reverse phase HPLC analysis of the conjugate sample. The percent free drug for either reaction was less than 2%. In addition, shorter conjugation reaction times are possible with SSNPP compared with SPP (U.S. Patent No.6, 913,748), which may decrease loss of some antibodies that are sensitive to prolonged exposure to organic solvent required in the conjugation reaction. Shorter reaction times should also decrease drug loss due to DM1 dimerization, which is a competing side reaction during conjugation. The resulting increases in yield and reduced side reactions should further contribute to reduced DM1 requirements.

[240] The enhanced rate and efficiency of conjugation when using SSNPP was also observed when conjugating a different drug to the antibody demonstrating the broad applicability of this new linker reagent. A comparison of conjugation efficiencies using SSNPP and SPP when conjugating the N901 antibody with the DNA-alkylating drug, DC4, a CC-1065 analogue, is shown, for example, in Table 3. By 2 hours the reaction using the SSNPP cross-linking reagent was complete whereas the reaction using the SPP reagent showed only 73% completeness by 2 hours and significant incorporation of drug beyond 2 hours (91% after 18 hours). Only much prolonged reaction times may lead to 100%) completeness.

Example 3. In vitro Cytotoxicity Evaluation of Maytansinoid Conjugates of Antibodies with Thioether (Non-Cleavable) and Disulfide Linkers Containing sulfonate group

[241] The cytotoxic effects of the antibody-maytansinoid conjugates with thioether and disulfide linkers containing a sulfonate group_were typically evaluated using a WST-8 cell-viability assay after a 4-5 day continuous incubation of the cancer cells with the conjugates. The antigen-expressing cancer cells (-1000-5000 cells per well) were incubated in 96-well plates in regular growth medium containing fetal bovine serum with various concentrations of the antibody-maytansinoid conjugates for about 5 days. The WST-8 reagent was then added and the plate absorbance was measured at 450 nm after ~2-5 h. The survival fraction was plotted versus conjugate concentration to determine the 7C ₅ o value (50% cell killing concentration) of the conjugate.

[242] Figures 60 and 61 show the enhancement in cytotoxicities of Anti-CanAg

(huC242) - maytansinoid conjugates with the sulfonate-containing disulfide-bonded linker (huC242-Sulfo-SPDB-DM4) bearing 6.0 to 7.6 maytansinoid/ Ab compared to the conjugate with 3.3 maytansinoid/Ab toward CanAg-positive COLO205 and COLO205-MDR cells. The potency of the conjugates with high maytansinoids loads indicate that the decoration of the antibody with up to 8 maytansinoid molecules did not affect the conjugate binding to the target COLO205 cells.

[243] Figure 64 shows the cytotoxic activities of anti-CanAg Ab-maytansinoid conjugates with similar maytansinoid load against CanAg antigen-positive COLO205-MDR cells. The presence of sulfonate group in disulfide linker significantly enhanced conjugate potency toward these multiple drug resistant cells. The enhanced potency of the sulfonate- linked conjugate is a novel finding and potentially very promising for therapeutic applications.

[244] Figure 63 shows the cytotoxic activities of anti-EpCAM Ab-maytansinoid conjugates with similar maytansinoid load against EpCAM antigen-positive COLO205-MDR cells. The presence of a sulfonate group in disulfide linker significantly enhanced conjugate potency toward these multiple drug resistant cells. The enhanced potency of the sulfonate- linked conjugate is a novel finding and potentially very promising for therapeutic

applications.

[245] Figure 64 shows the cytotoxic activities of anti-EpCAM Ab-maytansinoid conjugates with similar maytansinoid load against EpCAM antigen-positive HCT cells. The presence of a sulfonate group in the disulfide linker significantly enhanced conjugate potency toward these multiple drug resistant cells. The enhanced potency of the sulfonate-linked conjugate is a novel finding and potentially very promising for therapeutic applications.

[246] Figure 65 shows the cytotoxic activities of anti-EpCAM Ab-maytansinoid conjugates with similar maytansinoid load against EpCAM antigen-positive COLO205-MDR cells. The presence of a sulfonate group in the thioether linker significantly enhanced conjugate potency toward these multiple drug resistant cells. The enhanced potency of the sulfonate-linked conjugate is a novel finding and potentially very promising for therapeutic applications.

Example 4. Comparison of in vivo anti-tumor activity of the anti-EpCAM- maytansinoid conjugates, B38.1-SPDB-DM4 and B38.1-sulfo-SPDB-DM4, on colon cancer, COLO205 and COLO205-MDR, xenografts [247] The anti-tumor effect of B38.1-SPDB-DM4 and B38.1-sulfo-SPDB-DM4 conjugates was evaluated in a xenograft model of human colon carcinoma, COLO205 and COLO205-MDR, which was engineered to overexpress P-glycoprotein. The cells were injected subcutaneously in the area under the right shoulder of SCID mice. When the tumor's volume reached approximately 200 mm ³ in size, the mice were randomized by tumor volume and divided into three groups. Each group was treated with a single i.v. bolus of either B38.1-SPDB-DM4 (10 mg conjugate protein/kg), B38.1-sulfo-SPDB-DM4 (10 mg conjugate protein/kg) or phosphate-buffered saline (vehicle control). Tumor growth was monitored by measuring tumor size twice per week. Tumor size was calculated with the formula: length x width x height x ½.

[248] The changes in volumes of individual COLO205-MDR tumors are shown in

Figure 66. Treatment with either conjugate resulted in significant tumor growth delay.

B38.1-sulfo-SPDB-DM4 was more efficacious than B38.1-sulfo-SPDB-DM4 in this human colon cancer xenograft model.

[249] The changes in volumes of individual COLO205 tumors are shown in Figure

67. Treatment with either conjugated resulted in significant tumor growth delay. Two of six animals treated with B38.1 -sulfo-SPDB-DM4 had complete tumor regressions. Thus, B38.1 - sulfo-SPDB-DM4 was significantly more efficacious than B38.1-sulfo-SPDB-DM4 in this model.

Example 5. Synthesis of procharged linkers (CXl-1) -Gly-Gly-Gly-β -Ala-OtBu

[250] 1.3 g (4.0 mmol) of Z-Gly-Gly-Gly-OH, 0.583 g (4.0 mmol) of tert-butyl-3- aminopropionate 0.651 g (4.25 mmol) of hydroxybenzotriazole and 0.81 g (4.23 mmol) of N- (3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride were weighed into a 50 mL flask then dissolved in 20 mL of dimethylformamide with magnetic stirring under a nitrogen atmosphere. After 3 hours the reaction mixture was purified in 5 mL portions by reverse phase HPLC using a 5.0 cm x 25 cm C18 column. The column was run at 100 mL/min with deionized water containing 0.3 % formic acid 5% acetonitrile for 10 min followed by a 15 min linear gradient from 5% acetonitrile to 90% acetonitrile. Product fractions (retention time of 19 min) were combined and solvent was removed by rotary evaporation under vacuum to give 1.35 g (75%) of the title compound. 1H NMR (d ₆ -DMSO) 8.16 (t, J = 5.2 Ηζ, ΙΗ), 8.10 (t, J = 5.2 Hz, 1H), 7.82 (t, J = 5.2 Hz, 1H), 7.25 - 7.4 (m, 5H), 5.04 (s, 2H), 3.74 (d, J= 5.6 Hz, 2H), 3.67 (t, J= 6.4 Hz, 4H), 3.25 (q, J= 6.1 Hz, 2H), 2.35 (t, J= 6.8 Hz, 2H), 1.39 (s, 9H). ¹³ C NMR (d ₆ -DMSO) 170.45, 169.61, 169.00, 168.63, 156.49, 136.94, 128.30, 127.76, 127.69, 79.89, 65.51, 43.56, 42.10, 41.90, 34.89, 34.78, 27.70. HRMS ( M +Na ⁺ ) Calc. 473.2012 found 473.1995.

H-Gly-Gly-Gly-P-Ala-OtBu

[251] 1.3 g (2.89 mmol) of Z-Gly-Gly-Gly-p-Ala-OtBu was disolved in 80 mL of

95:5 methanol :deionized water in a 250 mL parr shaker flask to which was added 0.12 g of 10%> palladium on carbon. The flask was shaken under a hydrogen atmosphere (42 PSI) for 7 hours. The mixture was vacuum filtered through celite filter aid and the filtrate was concentrated by rotary evaporation under vacuum to give 0.88 g (96%) of the title compound. 1H NMR (d ₆ -DMSO) 8.12 (t, J = 1.6Hz 2H), 8.08 (t, J =1.6 Hz, 1H), 3.75 (s,2H), 3.64 (d, J = 5.9 2H), 3.28 (bs, 2H), 3.24 (q, J= 6.0 Hz, 2H), 3.13 (s, 2H), 2.35 (t, J= 6.8 Hz, 2H), 1.39 (s, 9H). ¹³ C NMR (d ₆ -DMSO) 173.38, 170.46, 169.18, 168.70, 79.89, 44.65, 41.95, 34.88, 34.78, 27.71. HRMS (M + H ⁺ ) Calc. 317.1825, found 317.1801

Mal-Gaba-Gly-Gly-Gly-β -Ala-OtBu

[252] 513 mg (2.8 mmol) of 4-(2,5-dioxo-2,5-dihydro-lH-pyrrol-l-yl)butanoic acid,

800 mg (0.2.8 mmol) tert-butyl 3-(2-(2-(2-aminoacetamido)acetamido)acetamido)propanoate and 583 mg (3.0 mmol) N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride were disolved in 12 mL of dimethyl formamide and stirred for 3 hours. The reaction mixture was purified in four equal portions by reverse phase HPLC using a 5.0 cm x 25 cm CI 8 column. The column was eluted at 100 mL/min with deionized water containing 0.3 % formic acid and 5% acetonitrile for 10 min followed by a 13 min linear gradient from 5% acetonitrile to 33 % acetonitrile. Product fractions (retention time of 21 min) were combined and solvent was removed by rotary evaporation under vacuum to give 832 mg (62 %) of the title compound. 1H NMR (d ₆ -DMSO) 8.10-8.16 (m, 2H), 8.07 (t, J = 4.8 Hz, 1H), 7.0 - 7.15(m, 1H), 3.747 (t, J = 6.0 Hz, 3H), 3.64 (d, J = 5.6 Hz, 2H), 3.41 (t, J = 6.8, 2H), 3.1- 3.33 (m, 1H), 3.19-3.26 (m, 2H), 2.348 (t, J = 6.8, 2H), 2.132 (t, J - 7.2 Hz, 2H), 1.67 - 1.76 (m, 2H), 1.39 (s, 9H). ¹³ C NMR (d ₆ -DMSO) 171.80, 170.98, 170.39, 169.48, 168.96, 168.56, 134.37, 79.83, 42.05, 41.83, 37.38, 34.82, 34.71 , 32.26, 27.83, 23.95. HRMS (M + Na ⁺ ) Calc. 504.2070 found 504.2046

Mal-Gaba-Gly-Gly-Gly-β -Ala-OH

[253] 820 mg (1.7 mmol) of Mal-Gaba-Gly-Gly-Gly-P-Ala-OtBu was disolved in

9.0 mL of 95:5 trifluoroacetic acid: deionized water and magnetically stirred for 3 hours. Solvent was removed by rotary evaporation under vacuum to give 730 mg (100%) of the title compound. 1H NMR (d ₆ -DMSO) 12.1 (bs, 1 H), 8.05-8.20 (m, 3H), 7.82 (t, J - 6.0 Hz, 1 H), 7.00 (s, 2H), 3.71 (t, J = 6.0 Hz, 4H), 3.65 (d, J - 6.0 Hz, 2H), 3.41 (t, J - 7.2 Hz, 2H), 3.26 (q, J- 5.6 Hz, 2H), 2.38 (t, J = 7.2 Hz, 2H,), 2.14 (q, J = 8.0 Hz, 2H), 1.67-1.77 (m, 2H). ¹³ C NMR (d ₆ -DMSO) 172.70, 171.83, 171.01, 169.50, 168.99, 168.51 , 134.38, 42.07, 41.84, 36.75, 34.70, 33.69, 32.28, 23.97 HRMS (M + Na ⁺ ) Calc. 448.1444 found 448.1465

Mal-Gaba-Gly-Gly-Gly-β -Ala-ONHS (CXl-1)

[254] 76 mg (0.18 mmol) of Mal-Gaba-Gly-Gly-Gly-p-Ala-OH, 72 mg, (0.376 mmol) of iV-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and 66 mg (0.575 mmol) of N-hydroxysuccinimide were disolved in 1.0 mL of dimethylformamide with magnetic stirring. After 2 hours the reaction mixture was purified in two equal portions by reverse phase HPLC using a 1.9 cm x 10 cm C8 column. The column was eluted at 18 mL/min with deionized water containing 0.3 % formic acid and 5% 1,4-dioxane for 3 min followed by a 15 min linear gradient from 5% 1,4-dioxane to 30 % 1,4-dioxane. Product fractions (retention time 6.5 min) were collected in a flask and immediately frozen in a dry ice acetone bath. Solvent was removed by lyophilization at ambient temperature to give 40 mg (42%) of the title compound. 1H NMR (d ₆ -DMSO) 8.08-8.1 1 (m, 3H), 7.99 (t, J = 6.4 Ηζ,ΙΗ), 7.00 (s, 2H), 3.6-3.75 (m, 6H), 3.0-3.2 (m, 4H), 2.84 ( s, 4H), 2.13 (t, J = 7.6 Hz), 1.83-1.93 (m, 2H), 1.69-1.72 (m, 2H). HRMS (M + Na+) calc. 545.1608 found 545.1638

Z-Glu(OtBu)-Gl _y- Gly-NH ₂

[255] 40 mL of Dimethyl formamide was added to 2.52 g (7.47 mmol) of Z-

Glu(OtBu)-OH, 1.3 g (8.49 mmol) of hydroxybenzotriazole, 1 .3 g (7.76 mmol) of ! !-Giy- GlyNH2, and 1.52 g ( 7.93 mmol) of N-(3-dimethylammopropyl)-N'-ethylcarbodiimide hydrochloride. 2.5 mL (14.3 mmol) of diisopropyl ethyl amine was added and the reaction was stirred over night. The reaction mixture was purified in three equal portions by direct injection on a preparative 5 cm x 25 cm C 18 HPLC column. The column was run at 100 mL/min with deionized water containing 0.3 % formic acid with 5% acetonitrile for 10 min followed by a 15 min linear gradient from 5% acetonitrile to 90% acetonitrile. Product fractions (retention time 18 - 20 min) were combined and solvent was removed by rotary evaporation under vacuum to give 2.9 g (83%) of the title compound. Ή NMR (400 MHz, CDC1 ₃ ) 5 7.79 - 7.68 (m, 1H), 7.64 (s, 1H), 7.27 (q, J = 4.9, 5H), 6.90 (s, 1H), 6.42 (s, 1H), 6.35 (d, J = 6.8, 1H), 5.08 (d, J = 12.0, 1H), 4.98 (d, J = 12.2, 1H), 4.20 (dd, J - 12.9, 7.6, 1H), 3.84-3.95 (m, 2H), 3.83 (d, J = 5.0, 2H), 2.42 - 2.19 (m, 2H), 2.07 (d, J= 6.9, 1H), 1.96 - 1.83 (m, 1H), 1.39 (s, 9H). ¹³ C NMR (101 MHz, DMSO) δ 171.79, 171.65, 170.82, 168.87, 163.04, 156.08, 136.86, 128.31, 127.74, 79.64, 65.58, 53.96, 42.17, 41.81, 31.25, 27.73, 27.01.

H-Glu(OtBu)-Gly-Gly-NH ₂

[256] 940 mg (2.09 mmol) of Z-Glu(OtBu)-Gly-GlyNH2 was dissolved in 40 mL of

95:5 methanol: de-ionized water in a 250 mL glass PARR hydrogenation shaker flak. 222 mg of 10% palladium on carbon was added to the flask and the contents were hydrogenated with shaking under hydrogen (40 PSI) for 4 hours. The mixture was vacuum filtered though celite filter aid and solvent was removed from the filtrate by rotary evaporation to give 640 mg (94%) of the title compound. Ή NMR (400 MHz, DMSO) δ 4.03 (s, 1H), 3.75 (d, J = 3.3, 2H), 3.63 (s, 2H), 3.30 - 3.22 (m, J= 3.6, 1H), 3.14 - 3.10 (m, 1H), 2.27 (t, J= 7.9, 2H), 1.84 (td, J = 13.6, 7.4, 1H), 1.63 (td, J = 15.0, 7.5, 1H), 1.39 (s, 9H). ¹³ C NMR (101 MHz, MeOD) δ 176.53, 174.24, 172.00, 170.32, 81.82, 55.21, 43.64, 43.16, 40.44, 32.31, 30.45, 28.41. HRMS (M + H ⁺ ) Calc. 317.1825 found 317.1800.

E001008-28 Mal-Gaba-Glu(OtBu)-Gly-Gly-NH ₂

[257] 603 mg (1.9 mmol) of H-Glu(OtBu)-Gly-Gly-NH2, 372 mg (2.03 mmol) of

Mal-Gaba-OH and 430 mg (2.24 mmol) of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride were dissolved in 4.5 mL of dimethyl formamide and 800 ΐ of dichloromethane. The reaction was stirred for 3 hours at ambient temperature. The reaction mixture was purified in two equal portions by direct injection on a preparative 5 cm x 25 cm CI 8 HPLC column. The column was run at 100 mL/min with deionized water containing 0.3 % formic acid 5% acetonitrile for 10 min followed by a 15 min linear gradient from 5% acetonitrile to 90% acetonitrile. Product fractions (retention time 17.4 - 19.2 min) were combined and solvent was removed by rotary evaporation under vacuum to give 2.9 g (83%) of the title compound. Ή NMR (400 MHz, CDC1 ₃ ) δ 8.16 (t, J = 5.7, 1H), 8.06 (d, J - 7.4, 1H), 7.99 (t, J = 5.8, 1H), 7.19 (s, 1H), 7.06 (s, 2H), 4.18 (dd, J = 13.4, 7.9, 1H), 3.70 (d, J = 5.7, 2H), 3.62 (d, J = 5.8, 2H), 3.42 - 3.37 (m, 2H), 2.23 (t, J = 8.0, 2H), 2.12 (dd, J = 8.1, 6.4, 2H), 1.87 (dt, J = 14.2, 7.9, 1H), 1.70 (dt, J = 13.7, 6.8, 2H), 1.38 (s, 9H). ¹³ C NMR (101 MHz, DMSO) δ 173.12, 171.77, 171.65, 171.03, 170.79, 168.89, 134.43, 79.62, 52.02, 42.14, 41.81, 36.80, 32.29, 31.22, 27.73, 26.95, 24.02. HRMS (M + Na ⁺ ) Calc. 504.2070 found 504.2053.

Mal-Gaba-Glu(OH)-Gly-Gly-NH ₂

[258] 105 mg (0.218 mmol) of Mal-Gaba-Glu(OtBu)-Gly-Gly-NH2 was dissolved in 5 mL of 95:5 trifluoroacetic acid:de-ionized water and magnetically stirred for 2 hours. Solvent was removed by rotary evaporation and residue was taken up in 6 mL acetonitrile + 1.5 mL toluene to give a suspension. Solvent was evaporated from the suspension by rotary evaporation under vacuum to give 92 mg (100%) of the title compound. Ή NMR (400 MHz, DMSO) δ 6.99 (s, 2H), 4.18 (dd, J= 8.2, 5.7, 1H), 3.70 (s, 2H), 3.61 (s, 2H), 3.40 (t, J= 6.8, 2H), 2.26 (t, J = 7.8, 2H), 2.19 - 2.05 (m, 2H), 1.90 (dt, J = 13.7, 7.4, 1H), 1.73 (dt, J = 14.2, 7.5, 3H). ¹³ C NMR (101 MHz, DMSO) δ 173.76, 171.72, 170.99, 170.70, 168.81 , 134.37, 52.00, 41.97, 41.63, 36.75, 32.19, 29.95, 26.79, 23.93. Mal-Gaba-G!u(ONHS)-Gly-Gly-NH ₂

[259J 94 mg (0,22 mmol) of MaI-Gaba-Glu(OH)-Gly-Gly-NH ₂ , 75 mg (0.65 mmol)

N-hydroxysucciiiimide and 1 10 mg (0.57 mmol) of N-(3-dimethylarninopropyl)-N'- ethylcarbodiirnide hydrochloride were magnetically stirred in 1 raL of dimethyl formamide doe 3 hours. The crude reaction mixture was purified in three equal portions by direct injection on a 1 ,9 cm x 10 cm C8 column. The column was run at 18 mL/min with deionized water containing 0.3% formic acid and 5% l ,4~dioxane for 3 min followed by an 18 min linear gradient from 5% L4~dioxane to 30% l,4~dioxarse. Product fractions (retention time 7.3 min) were collected in a flask and immediately frozen in a dry ice/acetone bath. The combined frozen material was lyophilized to give 80 mg (70 %) of the title compound. ^f H NMR (400 MHz, DMSO) 8 8.20 (t, J = 5.4, 1H), 8.13 (d, J = 13, 1H), 8.03 ( J = 5.6, 1H), 7.21 (s, 1H), 7.06 (s, 1H), 7.01 (s, 2H), 4.29 (dd, J= 13.7, 6.5, 1 H), 3.84 - 3.69 (m, 2H), 3.63 id, J - 5.7, 2H), 3.57 (s, 2H), 3.41 (t J = 6.8, 2H), 2.81 (s, 3H), 2.78 - 2.69 (m, 2H), 2.15 (dd, J= 9.1 , 6.2 _; 1 H), 2.10 - 1.95 (m, I B), 1.88 (dt J= 17.0, 7.5, 1H), 1.73 (dd, J- 14.0, 6.9, 2H). HRMS (M + Na ⁺ ) Calc. 545.160S found 545.1627.

Example 6. Synthesis of positively charged linker

3-(Dimethylamino)dihydrothiophen-2(3H)-one (217).

[260] 3-aminodihydrothiophen-2(3H)-one hydrochloride (213) (1.0 g, 6.51 mmol) and formaldehyde (3 ml, 40.3 mmol) in methanol was added sodium cynoboronhydride (0.409 g, 6.51 mmol) in five portion in 1 h. After stirred for 2 h, the mixture was evaporated, redissolved in EtAc, washed with 1 M NaH2P04, dried over MgS04, filtered, concentrated and purified by Si0 ₂ column eluted with MeOH/DCM (1 :30) to afford 0.812 g (86%) of the title compound. 1H NMR (CDC1 ₃ ) 3.49 (dd, 1H, J = 6.3, 12.1 Hz), 3.24 (m, 2H), 2.42 (s, 6H), 2.38 (m, 1H), 2.21 (m, 1H); 13C NMR 206.58, 73.24, 41.62, 27.47, 25.51; ESI MS m/z+146.0 (M +H), 168.0 (M +Na).

[261] 2-(dimethylamino)-4-(pyridin-2-yldisulfanyl)butanoic acid (218).

[262] 3-(dimethylamino)dihydrothiophen-2(3H)-one (217) (0.95 g, 6.54 mmol) was stirred at 15 ml of 0.5 M NaOH and 10 ml of methanol solution for 30 min, nutralized with H3PO4 to pH 7.2, added 1 ,2-di(pyridin-2-yl)disulfane (5.76 g, 26.2 mmol) in 50 ml of methanol, stirred overnight, concentrated, washed with EtAc and the aquoues solution was loaded on C-18 column, eluted from 5% methanol in 0.01% formic acid to 30% methanol in 0.01% formic acid to afford the title product (368 mg, 20.65 % yield). Ή NMR (CDl ₃ OD) 8.31 (dd, 1H, J = 0.7, 4.7 Hz), 7.77 (m, 2H), 7.15 (dd, 1H, J - 0.8, 5.8 Hz), 3.22 (m, 1H), 2.85 (m, 2H), 2.51 (s, 6H), 2.05 (m, 2H); ¹³ C NMR 175.00, 161.28, 150.46, 139.40, 122.60, 121.49, 71.20, 42.46, 36.29, 29.88; ESI MS m/z+ 272.9 (M + H), 295.0 (M+Na).

[263] 2,5-dioxopyrrolidin-l-yl 2-(dimethylamino)-4-(pyridin-2- yldisulfanyl)butanoate (219)

[264] 2-(dimethylamino)-4-(pyridin-2-yldisulfanyl)butanoic acid (218) (92 mg,

0.338 mmol), l-hydroxypyrrolidine-2,5-dione (65 mg, 0.565 mmol) and EDC (185 mg, 0.965 mmol) was stirred in 3 ml of DMA at 50°C overnight, evaporated and purified on Si0 ₂ column eluted froml : 10 to 1 :4 of methanol/CH ₂ Cl ₂ to afford 43 mg (35%) of the title product. Ή NMR (CDl ₃ OD) 8.40 (m, 1H), 7.83 (m, 2H), 7.22 (m, 1H), 3.34 (m, 1H), 2.82 (m, 2H), 2.75 (s, 4H), 2.66 (s, 6H), 1.98 (m, 2H); ¹³ C NMR 177.21, 161.78, 161.12, 150.68, 139.37, 122.70, 121.66, 70.80, 44.16, 43.15, 36.06, 27.38; ESI MS m/z+ 369.2 (M + H).

Example 7. Preparation of huMy9-6-CXl-l-DMl procharged linker conjugates [265] The following stock solutions were used: 39.6 mM DM1 in DMA; (2) 17.8 mM solution of CXl-1 linker in DMA; (3) 200 mM succinate buffer pH 5.0 with 2 mM EDTA were used.. The reaction mixture containing between 8, 12 or 16 equivalents of linker to antibody were added to a solution of the antibody at 4 mg/ml in 90% phosphate buffer pH 6.5)/ 10% DMA and allowed to react for 2h at 25°C. pH 5.0, followed by reaction with DM1.

[266] The Ab conjugate was separated from excess small molecule reactants using a

G25 column equilibrated in PBS pH 7.4. The purified conjugate was allowed to hold for 2d at 25'C to allow any labile drug linkages to hydrolyze and then the conjugate was further purified from free drug by dialysis in PBS overnight, and then 10 mM histidine/130 mM glycine buffer pH 5.5 (lx o/n). The dialyzed conjugate was filtered using a 0.2 um filter and assayed by UV/Vis to calculate number of maytansinoids per Ab using known extinction coefficients for maytansinoid and antibody at 252 and 280 nm. The recovery was ~-70% and number of maytansinoids/antibody measured for each conjugate ranged from 3.7 to 6.8 depending on the linker excess used.

Table 1. Comparison of SSNPP and SPP linker in the conjugation of N901 antibody with DM1. Conjugation was conducted for 2 hours at the indicated pH using a 1.7-fold molar excess of DM1 per linker.

Reduced linker to antibody ratio required to reach target DM1 to antibody ratio with SSNPP as linker. Conjugation was conducted for 2 hours at pH 7.4 using a 1.1 -fold molar excess of DM1 per linker.

I Linker Linker/Ab DMl/Ab

i

Comparison of SSNPP and SPP linker in the conjugation of N901 antibody with DC4. Conjugation was conducted for the indicated time at pH 7.4 using a 1.4-fold molar excess of DC4 per linker.

Example 8. Introduction of a charged linker reduces DM4 toxicity in antibody drug conjugates

[267] Previous studies have shown that at or near 4 mg/kg in rabbit model and human clinical trials, antibody drug conjugates of DM4 comprising non-charged linkers produce ocular toxicity, causing dose reduction in the clinical trials and discontinuation of treatment. To determine whether inclusion of a charged linker can decrease ocular toxicity, antibody drug conjugates comprising either N-succinimidyl 4-(2-pyridyldithio)butanoate (SPDB) or N-succinimidyl 4-(2-pyridyldithio)-2-sulfobutanoate (sulfo-SPDB) linked to the antibody huMovl 9 (M9346A) and DM4 were generated. The antibody huMovl9 (M9346A) is described in US Appl. Pub. No. 2012/0009181 , which is herein incorporated by reference.

[268] Table 4 demonstrates that administration of the substituted charged sulfo-

SPDB linker for the uncharged SPDB linker greatly decreases ocular toxicity in a rabbit model. A DM1 conjugate, which is known not to cause ocular toxicity at elevated administration levels, was included as a control. [269] Maytansinoid conjugates with different linker-maytansinoid formats were evaluated for induction of corneal ocular toxicity in a preclinical rabbit model. Hallmarks of corneal epithelial damage such as migration of pigmented basal epithelial cells distal from the limbus, corneal pannus, and epithelial erosion were assessed following 3 weekly doses.

(-), similar to control; (+), mild toxicity with pigmented cell migration into the cornea; (++), moderate toxicity with pigmented cell migration and corneal pannus; (++- ^; -), moderate toxicity with early onset; nd, not determined.

Example 9. Pharmacokinetics and toxicity of IMGN242 in human ciinicai trials.

[270] IMGN242 is an antibody drug conjugate for the treatment of CanAg- expressing tumors. The compound is made by conjugating the potent cytotoxic maytansinoid, DM4, to the monoclonal antibody, huC242. Forty-five patients have been treated with IMGN242 at 8 different dose levels (18 to 297 mg/m2) in two clinical trials. Dose limiting toxicities (DLTs) included decreased visual acuity, corneal deposits and keratitis, which appeared to improve in patients where fo! low-up data is available. A two-phase pharmacokinetic profile was observed for IMGN242 in plasma from patients with low circulating CanAg levels (<100Q U/ ^' mL), with an initial rapid distribution phase that lasted about 48 hours, followed by a slower terminal elimination phase. Preliminary pharmacokinetic analysis revealed an elimination phase half-life for IMGN242 of about 5 days for patients with low circulating CanAg. The detemined half-life in patieiits was similar to that predicted for IMGN242 from preclinical pharmacokinetic studies (tl/2 about 5 days in mice and 4 days in cynomolgus monkeys).

[271] Eleven patients were noted to have circulating CanAg levels greater than 1000

U/mL, although there appeared to be no correlation between high plasma CanAg and the pattern of tumor CanAg expression. High plasma CanAg levels appeared to have a marked impact on the pharmacokinetics of IMGN242 with clearance increased 3 to 5-fold in patients with high CanAg (>1000 U/mL) compared to patients with low levels (<1000 U/mL). Cmax increased proportionally with increasing dose and was not significantly affected by circulating CanAg levels. It appeared that patients who developed study drug-related ocular toxicities had low plasma CanAg levels which may correlate with higher IMGN242 exposure in these patients.

[272] The circulating CanAg level did not correlate with the tumor CanAg antigen expression in patients. The data is suggestive of a correlation between the level of plasma CanAg, IMGN242 exposure and the observed ocular toxicities in patients. In patients with low plasma CanAg levels (< 1000 U/ml), the dose of 168 mg/m2 appeared to be associated with a notable incidence of possible study drug-related ocular toxicities (Figures 72 and 73).

Example 10. Pharmacokinetics and toxicity of SAR3419 in human clinical trials.

[273] SAR3419 is a DM4-containing antibody drug conjugate that comprises the humanized antibody Hu-B4 (humanized mouse IgGl MAb targeting CD 19) and the SPDB linker. Phase I clinical trials were initiated in patients having relapsed or refractory CD 19+ B cell Non-Hodgkins Lymphoma. As shown in Figure 74, SAR3419 exposure increased with dose and was eliminated rapidly at both the 160 mg/m and 208 mg/m dosages. However, some patients which received SAR3419 at either dosage displayed ocular toxicity (Figure 75).