Title:
METHODS FOR DETERMINATION AND MONITORING OF TRANSPLANT REJECTION BY MEASURING RNA
Document Type and Number:
WIPO Patent Application WO/2023/244735
Kind Code:
A3
Abstract:
The present disclosure provides methods for preparation and analysis of biological samples of transplant recipients or subjects suffering from a disease or disorder, wherein the methods comprise extracting fragmented or intact RNA (such as mRNA or miRNA) derived from sample of the transplant recipient or subject suffering from a disease or disorder, wherein the extracted RNA comprises target RNA molecules preselected to enable assessment of transplant rejection. The detection of the target RNA molecules and total amount of RNA derived from the donor organ can be used to determine and/or monitoring transplant rejection.
Inventors:
MIR ALAIN (US)
BUTSKOVA ANASTASIA (US)
BRUNS AARON (US)
CHAULEAU MATHIEU (US)
AHMED EBAD (US)
VAN HUMMELEN PAUL (US)
MUGHAL FIZZA (US)
BUTSKOVA ANASTASIA (US)
BRUNS AARON (US)
CHAULEAU MATHIEU (US)
AHMED EBAD (US)
VAN HUMMELEN PAUL (US)
MUGHAL FIZZA (US)
Application Number:
PCT/US2023/025430
Publication Date:
January 18, 2024
Filing Date:
June 15, 2023
Export Citation:
Assignee:
NATERA INC (US)
International Classes:
C12Q1/6806; C12Q1/6883
Domestic Patent References:
| WO2020010255A1 | 2020-01-09 | |||
| WO2021243045A1 | 2021-12-02 |
Foreign References:
| US20160138106A1 | 2016-05-19 | |||
| KR20210024986A | 2021-03-08 |
Other References:
HARDIGAN ANDREW A ET AL: "CRISPR/Cas9-targeted removal of unwanted sequences from small-RNA sequencing libraries", NUCLEIC ACIDS RESEARCH, vol. 47, no. 14, 22 August 2019 (2019-08-22), GB, pages e84 - e84, XP093009588, ISSN: 0305-1048, Retrieved from the Internet DOI: 10.1093/nar/gkz425
BIN XIAO ET AL: "Plasma microRNA signature as a noninvasive biomarker for acute graft-versus-host disease", BLOOD, 1 September 2013 (2013-09-01), pages 3365 - 3375, XP055643518, Retrieved from the Internet
MAS V R ET AL: "MicroRNAs as Biomarkers in Solid Organ Transplantation", AMERICAN JOURNAL OF TRANSPLANTATION, BLACKWELL MUNKSGAARD, DK, vol. 13, no. 1, 8 November 2012 (2012-11-08), pages 11 - 19, XP072344236, ISSN: 1600-6135, DOI: 10.1111/J.1600-6143.2012.04313.X
BOSTJANCIC EMANUELA ET AL: "The Role of Immune-Related miRNAs in the Pathology of Kidney Transplantation", BIOMOLECULES, vol. 11, no. 8, 12 August 2021 (2021-08-12), pages 1198, XP093107082, Retrieved from the Internet DOI: 10.3390/biom11081198
BIN XIAO ET AL: "Plasma microRNA signature as a noninvasive biomarker for acute graft-versus-host disease", BLOOD, 1 September 2013 (2013-09-01), pages 3365 - 3375, XP055643518, Retrieved from the Internet
MAS V R ET AL: "MicroRNAs as Biomarkers in Solid Organ Transplantation", AMERICAN JOURNAL OF TRANSPLANTATION, BLACKWELL MUNKSGAARD, DK, vol. 13, no. 1, 8 November 2012 (2012-11-08), pages 11 - 19, XP072344236, ISSN: 1600-6135, DOI: 10.1111/J.1600-6143.2012.04313.X
BOSTJANCIC EMANUELA ET AL: "The Role of Immune-Related miRNAs in the Pathology of Kidney Transplantation", BIOMOLECULES, vol. 11, no. 8, 12 August 2021 (2021-08-12), pages 1198, XP093107082, Retrieved from the Internet
Download PDF:
Previous Patent: VOLUME INTERSECTION USING ROTATED BOUNDING VOLUMES
Next Patent: ASYMMETRIC POLYMERIC POROUS FILTER MEMBRANES AND RELATED METHODS
Next Patent: ASYMMETRIC POLYMERIC POROUS FILTER MEMBRANES AND RELATED METHODS