Title:
METHODS FOR THE IDENTIFICATION OF AGENTS THAT MODULATE THE STRUCTURE AND PROCESSING OF A MEMBRANE BOUND PRECURSOR PROTEIN
Document Type and Number:
WIPO Patent Application WO2004042074
Kind Code:
A3
Abstract:
The present invention provides methods for the screening and identification of agents from a large library of molecular structures that can alter the cleavage of a membrane protein of interest. Agents identified by the methods of the present invention that modify the cleavage of the membrane protein can be used in the treatment and prevention of diseases such as inflammation, diabetes, cancer, Alzheimer's disease, Parkinson's disease, and the like. The methods select for and identify effector agents that bind to the membrane protein of interest causing a structural change in the structure of the membrane protein in such a way that the efficiency of the cleavage of a secretase is modulated. Further, the methods are carried out in an in vivo system that provides for physiological conditions similar or identical to conditions for membrane protein processing. Agents can be selected for their ability to cause a decrease or increase the amount of secretase cleavage of the membrane protein.
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Inventors:
HAGEN FREDERICK S (US)
Application Number:
PCT/US2003/035289
Publication Date:
December 09, 2004
Filing Date:
November 04, 2003
Export Citation:
Assignee:
ICOGENEX CORP (US)
HAGEN FREDERICK S (US)
HAGEN FREDERICK S (US)
International Classes:
A61K49/00; C12N15/00; G01N33/53; G01N33/567; G01N33/68; C12Q; (IPC1-7): C12N15/00; G01N33/53; G01N33/567; A61K49/60
Foreign References:
| US6440698B1 | 2002-08-27 | |||
| US6175057B1 | 2001-01-16 | |||
| US20020127564A1 | 2002-09-12 | |||
| US6420110B1 | 2002-07-16 |
Other References:
HOOPER N.M. ET AL: "Membrane protein secretases", BIOCHEM J., vol. 321, 1997, pages 265 - 279, XP002903130
RIAN E. ET AL: "A Signal Sequence Trap Based on Cell Enrichment Using Anti-CD19 Antibody Coated Magnetic Beads", SCAND. J. IMMUNOL., vol. 54, September 2001 (2001-09-01), pages 280 - 284, XP002903131
PONCET C.: "CD24, a glycophosphatidylinositol-anchored molecule is transiently expressed during the development of human central nervous system and is a marker of human neural cell lineage tumors", ACTA NEUROPATHOL., vol. 91, no. 4, 1996, pages 400 - 408, XP001051641
MURTHY S.C. ET AL: "Characterization of the interleukin 3 receptor", EXP. HEMATOL., vol. 18, no. 1, January 1990 (1990-01-01), pages 11 - 17, XP002903300
MAZUR-KOLECKA B. ET AL: "Accumulation of Alzheimer amyloid-beta peptide in cultured myocytes is enhanced by serum and reduced by cerebrospinal fluid", J. NEUROPATHOL. EXP. NEUROL., vol. 56, no. 3, March 1997 (1997-03-01), pages 263 - 272, XP002903169
RIAN E. ET AL: "A Signal Sequence Trap Based on Cell Enrichment Using Anti-CD19 Antibody Coated Magnetic Beads", SCAND. J. IMMUNOL., vol. 54, September 2001 (2001-09-01), pages 280 - 284, XP002903131
PONCET C.: "CD24, a glycophosphatidylinositol-anchored molecule is transiently expressed during the development of human central nervous system and is a marker of human neural cell lineage tumors", ACTA NEUROPATHOL., vol. 91, no. 4, 1996, pages 400 - 408, XP001051641
MURTHY S.C. ET AL: "Characterization of the interleukin 3 receptor", EXP. HEMATOL., vol. 18, no. 1, January 1990 (1990-01-01), pages 11 - 17, XP002903300
MAZUR-KOLECKA B. ET AL: "Accumulation of Alzheimer amyloid-beta peptide in cultured myocytes is enhanced by serum and reduced by cerebrospinal fluid", J. NEUROPATHOL. EXP. NEUROL., vol. 56, no. 3, March 1997 (1997-03-01), pages 263 - 272, XP002903169
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