Title:
METHODS FOR IDENTIFYING VARIATIONS IN POLYNUCLEOTIDE SEQUENCES
Document Type and Number:
WIPO Patent Application WO1998044157
Kind Code:
A3
Abstract:
A step-wise integrated process for identifying sequence variations in polynucleotide sequences is disclosed. The identification process is composed of three stages, including allele specific hybridization assays of known sequence variations (Stage I), sequence variation locating assays (Stage II), and direct sequencing (Stage III). The methods can be used for efficient and accurate detection of mutations in any test gene sample.
Inventors:
MURPHY PATRICIA D
WHITE MARGA B
WHITE MARGA B
Application Number:
PCT/US1998/006002
Publication Date:
December 30, 1998
Filing Date:
March 26, 1998
Export Citation:
Assignee:
ONCORMED INC (US)
International Classes:
C12Q1/68; (IPC1-7): C12Q1/68
Other References:
MERAJVER ET AL.: "Risk assesment and presymptomatic molecular diagnosis in hereditary breast cancer.", DNA TECHNOLOGY, vol. 16, no. 1, March 1996 (1996-03-01), pages 139 - 167, XP002079197
HACIA J G ET AL: "DETECTION OF HETEROZYGOUS MUTATIONS IN BRCA1 USING HIGH DENSITY OLIGONUCLEOTIDE ARRAYS AND TWO-COLOUR FLUORESCENCE ANALYSIS", NATURE GENETICS, vol. 14, no. 4, December 1996 (1996-12-01), pages 441 - 447, XP000615885
HACIA J G ET AL: "DETECTION OF HETEROZYGOUS MUTATIONS IN BRCA1 USING HIGH DENSITY OLIGONUCLEOTIDE ARRAYS AND TWO-COLOUR FLUORESCENCE ANALYSIS", NATURE GENETICS, vol. 14, no. 4, December 1996 (1996-12-01), pages 441 - 447, XP000615885
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