Title:
METHODS AND KITS FOR ISOLATING TARGET NUCLEIC ACIDS BELOW A TARGET SIZE FROM A SAMPLE
Document Type and Number:
WIPO Patent Application WO/2024/093523
Kind Code:
A1
Abstract:
The present disclosure relates to methods, compositions, and kits for isolating target nucleic acids below a target size from a sample comprising nucleic acid components. In some embodiments, the methods involve one or more aqueous two-phase system (ATPS) compositions, at least one solid phase medium, and at least one buffer. Some embodiments provide a kit comprising one or more ATPS compositions, at least one solid phase medium, and at least one buffer. Other embodiments provide methods for diagnosing a disease or condition using the methods described herein.
Inventors:
SAINI VASU (CN)
CHUNG CHEUK YIU TENNY (CN)
BRADBURY DANIEL WILLIAM (CN)
KITTUR HARSHA MADAN (SG)
LAM CHEUK YIN (CN)
TSE KAR KEE (CN)
CHEUNG KIT (CN)
CHIU YIN TO (CN)
MOSLEY GARRETT LEE (CN)
CHUNG CHEUK YIU TENNY (CN)
BRADBURY DANIEL WILLIAM (CN)
KITTUR HARSHA MADAN (SG)
LAM CHEUK YIN (CN)
TSE KAR KEE (CN)
CHEUNG KIT (CN)
CHIU YIN TO (CN)
MOSLEY GARRETT LEE (CN)
Application Number:
PCT/CN2023/117658
Publication Date:
May 10, 2024
Filing Date:
September 08, 2023
Export Citation:
Assignee:
PHASE SCIENT INTERNATIONAL LTD (CN)
International Classes:
C12N15/10; C12Q1/6806; G01N33/543
Domestic Patent References:
| WO2021037075A1 | 2021-03-04 | |||
| WO2021185336A1 | 2021-09-23 | |||
| WO2022008591A1 | 2022-01-13 | |||
| WO2019118705A1 | 2019-06-20 | |||
| WO2019046563A1 | 2019-03-07 | |||
| WO2007140417A2 | 2007-12-06 | |||
| WO2021148393A1 | 2021-07-29 |
Foreign References:
| US20180258419A1 | 2018-09-13 | |||
| US20150166592A1 | 2015-06-18 |
Other References:
JANKU FILIP ET AL: "A novel method for liquid-phase extraction of cell-free DNA for detection of circulating tumor DNA", vol. 11, no. 1, 4 October 2021 (2021-10-04), XP093063759, Retrieved from the Internet DOI: 10.1038/s41598-021-98815-x
SHIN HYUNWOO ET AL: "Aqueous two-phase system to isolate extracellular vesicles from urine for prostate cancer diagnosis", PLOS ONE, vol. 13, no. 3, 27 March 2018 (2018-03-27), pages e0194818, XP093103976, DOI: 10.1371/journal.pone.0194818
PEREIRA MATHEUS M. ET AL: "Pre-treatment strategies based on aqueous two-phase systems comprising ionic liquids to improve the adrenal cancer diagnosis", JOURNAL OF MOLECULAR LIQUIDS, vol. 367, 22 September 2022 (2022-09-22), NL, pages 120409, XP093103949, ISSN: 0167-7322, DOI: 10.1016/j.molliq.2022.120409
MENDES MARIA S.M. ET AL: "Aqueous two-phase systems as multipurpose tools to improve biomarker analysis", SEPARATION AND PURIFICATION TECHNOLOGY, vol. 317, 17 April 2023 (2023-04-17), NL, pages 123875, XP093103947, ISSN: 1383-5866, DOI: 10.1016/j.seppur.2023.123875
SHIN HYUNWOO ET AL: "Aqueous two-phase system to isolate extracellular vesicles from urine for prostate cancer diagnosis", PLOS ONE, vol. 13, no. 3, 27 March 2018 (2018-03-27), pages e0194818, XP093103976, DOI: 10.1371/journal.pone.0194818
PEREIRA MATHEUS M. ET AL: "Pre-treatment strategies based on aqueous two-phase systems comprising ionic liquids to improve the adrenal cancer diagnosis", JOURNAL OF MOLECULAR LIQUIDS, vol. 367, 22 September 2022 (2022-09-22), NL, pages 120409, XP093103949, ISSN: 0167-7322, DOI: 10.1016/j.molliq.2022.120409
MENDES MARIA S.M. ET AL: "Aqueous two-phase systems as multipurpose tools to improve biomarker analysis", SEPARATION AND PURIFICATION TECHNOLOGY, vol. 317, 17 April 2023 (2023-04-17), NL, pages 123875, XP093103947, ISSN: 1383-5866, DOI: 10.1016/j.seppur.2023.123875
Attorney, Agent or Firm:
EAGLE IP (SHEN ZHEN) LIMITED (CN)
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