Title:
METHODS FOR MAKING DISULFIDE-RICH PEPTIDES AND PROTEINS
Document Type and Number:
WIPO Patent Application WO/2023/141224
Kind Code:
A3
Abstract:
In alternative embodiments, provided are methods that are reliable and scalable for making disulfide bond rich peptides and proteins. In alternative embodiments, provided are oxidation refolding methods to produce disulfide bond rich peptides and proteins. In alternative embodiments, methods as provided herein can be used to make any disulfide bond-containing proteins, including but not limited to: three finger neurotoxin peptides (such as for example, rec-α-Bungarotoxin (rec-αBtx), rec-α-Cobratoxin (rec-αCTX), κ-Bungarotoxin(rec-κBtx), rec-MTa, rec-hannalgesin, rec-Mambalgin, rec-Slurp, rec-Pate), antibodies and antibody fragments (such as single chain antibody), extracellular domain of viral membrane proteins, cell surface receptors, other disulfide-bond rich toxin peptides (such as dendrotoxin, conotoxin) and the like.
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Inventors:
XU JIANG (US)
CHEN LIN (US)
CHEN LIN (US)
Application Number:
PCT/US2023/011168
Publication Date:
October 19, 2023
Filing Date:
January 19, 2023
Export Citation:
Assignee:
UNIV SOUTHERN CALIFORNIA (US)
International Classes:
C07K1/113; C07K1/14; C07K1/16; C07K1/36; C12N15/70
Domestic Patent References:
| WO2003097669A2 | 2003-11-27 |
Other References:
NELSON CHRISTOPHER A., LEE CHUNG A., FREMONT DAVED H.: "Oxidative Refolding from Inclusion Bodies : Methods and Protocols", STRUCTURAL GENOMICS AND DRUG DISCOVERY : METHODS AND PROTOCOLS, SPRINGER NEW YORK, NEW YORK, NY, vol. 1140, 1 January 2014 (2014-01-01), New York, NY, pages 145 - 157, XP009549721, ISSN: 1064-3745, ISBN: 978-1-4939-0354-2, DOI: 10.1007/978-1-4939-0354-2_11
XU JIANG, LI JIA, WU XINGAN, SONG CHEN, LIN YE, SHEN YOUXIAO, YE WEI, SUN CHENJING, WANG XIANNI, LI ZHICHAO, LIU YI, WEI LONGXIAO,: "Expression and refolding of bioactive α-bungarotoxin V31 in E. coli", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA., vol. 110, 1 June 2015 (2015-06-01), SAN DIEGO, CA. , pages 30 - 36, XP093101689, ISSN: 1046-5928, DOI: 10.1016/j.pep.2014.12.016
ANUPAM SINGH;VAIBHAV UPADHYAY;ARUN KUMAR UPADHYAY;SURINDER MOHAN SINGH;AMULYA KUMAR PANDA: "Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process", MICROBIAL CELL FACTORIES, SPRINGER, vol. 14, no. 1, 25 March 2015 (2015-03-25), pages 41, XP021220426, ISSN: 1475-2859, DOI: 10.1186/s12934-015-0222-8
XU JIANG, LEI XIAO, LI AO, LI JUN, LI SHUXING, CHEN LIN: "Scalable production of recombinant three-finger proteins: from inclusion bodies to high quality molecular probes", BIORXIV, 12 April 2022 (2022-04-12), XP093101690, Retrieved from the Internet [retrieved on 20231115], DOI: 10.1101/2022.04.11.487962
XU JIANG, LI JIA, WU XINGAN, SONG CHEN, LIN YE, SHEN YOUXIAO, YE WEI, SUN CHENJING, WANG XIANNI, LI ZHICHAO, LIU YI, WEI LONGXIAO,: "Expression and refolding of bioactive α-bungarotoxin V31 in E. coli", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA., vol. 110, 1 June 2015 (2015-06-01), SAN DIEGO, CA. , pages 30 - 36, XP093101689, ISSN: 1046-5928, DOI: 10.1016/j.pep.2014.12.016
ANUPAM SINGH;VAIBHAV UPADHYAY;ARUN KUMAR UPADHYAY;SURINDER MOHAN SINGH;AMULYA KUMAR PANDA: "Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process", MICROBIAL CELL FACTORIES, SPRINGER, vol. 14, no. 1, 25 March 2015 (2015-03-25), pages 41, XP021220426, ISSN: 1475-2859, DOI: 10.1186/s12934-015-0222-8
XU JIANG, LEI XIAO, LI AO, LI JUN, LI SHUXING, CHEN LIN: "Scalable production of recombinant three-finger proteins: from inclusion bodies to high quality molecular probes", BIORXIV, 12 April 2022 (2022-04-12), XP093101690, Retrieved from the Internet
Attorney, Agent or Firm:
EINHORN, Gregory P. (US)
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