Title:
METHODS FOR PREPARING MATURE RED BLOOD CELLS IN VITRO
Document Type and Number:
WIPO Patent Application WO/2022/089606
Kind Code:
A1
Abstract:
Provided is a method for producing red blood cells, comprising the step of: (a) providing a population of Lin- CD34- hemopoietic progenitor cells; (b) culturing the population of Lin-CD34- hemopoietic progenitor cells from (a) in a first medium comprising a combination of an aryl hydrocarbon receptor antagonist, a HIF-1 modulator and a PPAR alpha agonist; and (c) culturing the population of cells obtained from (b) in a second medium comprising a combination of a vitamin, a methylxanthine compound, and a thyroid hormone receptor agonist, thereby producing red blood cells.
Inventors:
GAO XIAOFEI (CN)
Application Number:
PCT/CN2021/127603
Publication Date:
May 05, 2022
Filing Date:
October 29, 2021
Export Citation:
Assignee:
WESTLAKE THERAPEUTICS HANGZHOU CO LTD (CN)
International Classes:
C12N5/078
Foreign References:
| CN111154719A | 2020-05-15 | |||
| CN111373029A | 2020-07-03 | |||
| CN106214701A | 2016-12-14 | |||
| CN103667188A | 2014-03-26 | |||
| CN103509753A | 2014-01-15 |
Other References:
TAO L, TOGARRATI PP , CHOI KD , SUKNUNTHA K: "StemRegenin 1 selectively promotes expansion of Multipotent Hematopoietic Progenitors derived from Human Embryonic Stem Cells", JOURNAL OF STEM CELLS & REGENERATIVE MEDICINE, vol. 13, no. 2, 18 December 2017 (2017-12-18), pages 1 - 5, XP055926735, ISSN: 0973-7154
LEE HSIANG-YING, GAO XIAOFEI, BARRASA M. INMACULADA, LI HU, ELMES RUSSELL R., PETERS LUANNE L., LODISH HARVEY F.: "PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal", NATURE, NATURE PUBLISHING GROUP UK, LONDON, vol. 522, no. 7557, 1 June 2015 (2015-06-01), London, pages 474 - 477, XP055784064, ISSN: 0028-0836, DOI: 10.1038/nature14326
LEE HSIANG-YING; LODISH HARVEY F.; GAO XIAOFEI; LI WENBO; PLATT RANDY; BARRASA INMACULADA; ELMES RUSSELL; ROSENFELD MICHAEL G.: "Thyroid Hormone Receptor Beta and NCOA4 Regulate Terminal Erythrocyte Differentiation", BLOOD, AMERICAN SOCIETY OF HEMATOLOGY, US, vol. 126, no. 23, 3 December 2015 (2015-12-03), US , pages 3590, XP086639625, ISSN: 0006-4971, DOI: 10.1182/blood.V126.23.3590.3590
LEE HSIANG-YING, GAO XIAOFEI, BARRASA M. INMACULADA, LI HU, ELMES RUSSELL R., PETERS LUANNE L., LODISH HARVEY F.: "PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal", NATURE, NATURE PUBLISHING GROUP UK, LONDON, vol. 522, no. 7557, 1 June 2015 (2015-06-01), London, pages 474 - 477, XP055784064, ISSN: 0028-0836, DOI: 10.1038/nature14326
LEE HSIANG-YING; LODISH HARVEY F.; GAO XIAOFEI; LI WENBO; PLATT RANDY; BARRASA INMACULADA; ELMES RUSSELL; ROSENFELD MICHAEL G.: "Thyroid Hormone Receptor Beta and NCOA4 Regulate Terminal Erythrocyte Differentiation", BLOOD, AMERICAN SOCIETY OF HEMATOLOGY, US, vol. 126, no. 23, 3 December 2015 (2015-12-03), US , pages 3590, XP086639625, ISSN: 0006-4971, DOI: 10.1182/blood.V126.23.3590.3590
Attorney, Agent or Firm:
ZHONGZI LAW OFFICE (CN)
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