Title:
METHODS FOR PRODUCING MONOTERPENE INDOLE ALKALOIDS
Document Type and Number:
WIPO Patent Application WO/2023/222879
Kind Code:
A3
Abstract:
The present invention relates to microorganisms for producing monoterpene indole al-kaloids (MIAs) and derivatives thereof de novo, including halogenated MIAs and halo-genated derivatives thereof. Also provided herein are methods for producing MIAs and derivatives thereof de novo, in particular halogenated MIAs and derivatives thereof, in a 5 microorganism, as well as useful nucleic acids, vectors and host cells for performing the present methods.
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Inventors:
JENSEN MICHAEL KROGH (DK)
HANSEN LEA GRAM (DK)
ZHANG JIE (DK)
KEASLING JAY D (DK)
BRADLEY SAMUEL ALAN (DK)
D'AMBROSIO VASIL (DK)
MARCUSSEN NILS EMIL JUNGE (DK)
HANSEN LEA GRAM (DK)
ZHANG JIE (DK)
KEASLING JAY D (DK)
BRADLEY SAMUEL ALAN (DK)
D'AMBROSIO VASIL (DK)
MARCUSSEN NILS EMIL JUNGE (DK)
Application Number:
PCT/EP2023/063481
Publication Date:
December 28, 2023
Filing Date:
May 19, 2023
Export Citation:
Assignee:
UNIV DANMARKS TEKNISKE (DK)
International Classes:
C12N1/20; C12N1/18; C12N9/16; C12N9/24; C12P17/12; C12P17/18
Domestic Patent References:
| WO2020229516A1 | 2020-11-19 |
Other References:
YERKES N ET AL: "Substrate specificity and diastereoselectivity of strictosidine glucosidase, a key enzyme in monoterpene indole alkaloid biosynthesis", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, ELSEVIER, AMSTERDAM NL, vol. 18, no. 10, 15 May 2008 (2008-05-15), pages 3095 - 3098, XP022669507, ISSN: 0960-894X, [retrieved on 20071122], DOI: 10.1016/J.BMCL.2007.11.063
MISA JOSHUA ET AL: "Engineered Production of Strictosidine and Analogues in Yeast", ACS SYNTHETIC BIOLOGY, vol. 11, no. 4, 16 March 2022 (2022-03-16), Washington DC ,USA, pages 1639 - 1649, XP093081630, ISSN: 2161-5063, Retrieved from the Internet DOI: 10.1021/acssynbio.2c00037
STEPHANIE BROWN ET AL: "De novo production of the plant-derived alkaloid strictosidine in yeast", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 112, no. 11, 9 February 2015 (2015-02-09), pages 3205 - 3210, XP055556409, ISSN: 0027-8424, DOI: 10.1073/pnas.1423555112
"Advances in botanical research; ISSN 0065-2296 ; Vol. 68.2013", vol. 68, 27 July 2013, ELSEVIER, GB, ISBN: 978-0-12-408061-4, article VONNY SALIM ET AL: "Towards Complete Elucidation of Monoterpene Indole Alkaloid Biosynthesis Pathway", pages: 1 - 37, XP055588065, DOI: 10.1016/B978-0-12-408061-4.00001-8
LIU JIAQI ET AL: "Transcriptional Regulation and Transport of Terpenoid Indole Alkaloid in Catharanthus roseus: Exploration of New Research Directions", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 18, no. 1, 28 December 2016 (2016-12-28), pages 53 - 72, XP093008928, DOI: 10.3390/ijms18010053
MISA JOSHUA ET AL: "Engineered Production of Strictosidine and Analogues in Yeast", ACS SYNTHETIC BIOLOGY, vol. 11, no. 4, 16 March 2022 (2022-03-16), Washington DC ,USA, pages 1639 - 1649, XP093081630, ISSN: 2161-5063, Retrieved from the Internet
STEPHANIE BROWN ET AL: "De novo production of the plant-derived alkaloid strictosidine in yeast", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 112, no. 11, 9 February 2015 (2015-02-09), pages 3205 - 3210, XP055556409, ISSN: 0027-8424, DOI: 10.1073/pnas.1423555112
"Advances in botanical research; ISSN 0065-2296 ; Vol. 68.2013", vol. 68, 27 July 2013, ELSEVIER, GB, ISBN: 978-0-12-408061-4, article VONNY SALIM ET AL: "Towards Complete Elucidation of Monoterpene Indole Alkaloid Biosynthesis Pathway", pages: 1 - 37, XP055588065, DOI: 10.1016/B978-0-12-408061-4.00001-8
LIU JIAQI ET AL: "Transcriptional Regulation and Transport of Terpenoid Indole Alkaloid in Catharanthus roseus: Exploration of New Research Directions", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 18, no. 1, 28 December 2016 (2016-12-28), pages 53 - 72, XP093008928, DOI: 10.3390/ijms18010053
Attorney, Agent or Firm:
HØIBERG P/S (DK)
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