Title:
METHODS FOR PRODUCING RECOMBINANT FACTOR VIII CHAINS FROM NON-FILAMENTOUS FUNGI, THEIR FUNCTIONAL RECONSTITUTION AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2014/041483
Kind Code:
A3
Abstract:
The present disclosure relates specifically to a process of producing heavy chain peptide and/or light chain peptide of recombinant Factor VIII protein using Pichia pastoris expression system. The disclosure further relates to a process of producing a functional recombinant Factor VIII protein by reconstituting the Heavy chain and Light chain produced using said Pichia pastoris expression system. The said functional recombinant Factor VIII protein shows improved activity and therefore is used in the management of haemophilia.
Inventors:
ARUNACHALAM VIJAYALAKSHMI MOOKAMBESWARAN (IN)
JANAKIRAMAN VIGNESH NARASIMHAN (IN)
VENKATARAMAN KRISHNAN (IN)
REDDY SUDHEER REDDY ASWATHA (IN)
KUTTY SATHEESHKUMAR PADIKKARA (IN)
NAIR SUKESH CHANDRAN (IN)
SRIVASTAVA ALOK (IN)
JANAKIRAMAN VIGNESH NARASIMHAN (IN)
VENKATARAMAN KRISHNAN (IN)
REDDY SUDHEER REDDY ASWATHA (IN)
KUTTY SATHEESHKUMAR PADIKKARA (IN)
NAIR SUKESH CHANDRAN (IN)
SRIVASTAVA ALOK (IN)
Application Number:
PCT/IB2013/058449
Publication Date:
June 05, 2014
Filing Date:
September 11, 2013
Export Citation:
Assignee:
CT FOR BIOSEPARATION TECHNOLOGY VIT (IN)
CHRISTIAN MEDICAL COLLEGE (IN)
CHRISTIAN MEDICAL COLLEGE (IN)
International Classes:
A61K48/00; A61K38/37; C12N1/20
Foreign References:
| US20060099685A1 | 2006-05-11 | |||
| CN1361178A | 2002-07-31 | |||
| US20120052530A1 | 2012-03-01 |
Other References:
"Invitrogen. pPICZa A, B, and C Pichia expression vectors for selection on Zeocin and purification of secreted, recombinant proteins. Cat. no. V195-20. Manual part no. 25-0150", 7 July 2010 (2010-07-07), pages 1, 5, XP002565398, Retrieved from the Internet [retrieved on 20140402]
DATABASE GENBANK [online] 23 April 2008 (2008-04-23), "Extracellular expression vector pKanB alpha, complete sequence.", XP055243496, retrieved from NCBI Database accession no. EU285586
See also references of EP 2895199A4
DATABASE GENBANK [online] 23 April 2008 (2008-04-23), "Extracellular expression vector pKanB alpha, complete sequence.", XP055243496, retrieved from NCBI Database accession no. EU285586
See also references of EP 2895199A4
Attorney, Agent or Firm:
VIJAYAKRISHNAN, Sindhu et al. (Intellectual Property Attorneys4121/B, 6th Cross, 19A Main, HAL II Stage, Bangalore Karnataka 8, IN)
Download PDF: