Title:
METHODS OF PROFILING TRANSLATION RATE
Document Type and Number:
WIPO Patent Application WO/2021/202542
Kind Code:
A3
Abstract:
Provided are methods of determining RNA translation rate in a biological sample, for example by crosslinking an RNA binding protein (RBP) to an RNA in a biological sample; identifying an RNA-RNA binding protein (RBP) complex within the biological sample, wherein the RNA-RBP complex comprises an RNA fragment bound by the RNA binding protein; and profiling the RNA fragment bound by the RNA binding protein, thereby determining a translation rate of the RNA in the biological sample.
Inventors:
YEO EUGENE (US)
VAN NOSTRAND ERIC (US)
VAN NOSTRAND ERIC (US)
Application Number:
PCT/US2021/024904
Publication Date:
November 11, 2021
Filing Date:
March 30, 2021
Export Citation:
Assignee:
UNIV CALIFORNIA (US)
International Classes:
C07K14/00; C12N9/22; C12Q1/6855; C12Q1/6869; G01N33/539
Other References:
VANNOSTRAND ET AL.: "Robust transcriptome-wide discovery of RNA binding protein binding sites with enhanced CLIP (eCLIP)", NATURE METHODS, vol. 13, no. 6, June 2016 (2016-06-01), pages 508 - 514, XP055873058
JUNGKAMP ET AL.: "In Vivo and Transcriptome-wide Identification of RNA Binding Protein Target Sites", MOLECULAR CELL, vol. 44, no. 5, 16 November 2011 (2011-11-16), pages 828 - 840, XP028391776
JUNGKAMP ET AL.: "In Vivo and Transcriptome-wide Identification of RNA Binding Protein Target Sites", MOLECULAR CELL, vol. 44, no. 5, 16 November 2011 (2011-11-16), pages 828 - 840, XP028391776
Attorney, Agent or Firm:
YOON, Sohee Kim et al. (US)
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