Title:
METHODS FOR PROTECTING AGAINST LETHAL INFECTION WITH BACILLUS ANTHRACIS
Document Type and Number:
WIPO Patent Application WO2001045639
Kind Code:
A3
Abstract:
Methods of inducing an immune response which protects a susceptible animal subject from lethal infection with Bacillus anthracis (B. anthracis) are provided. One method comprises administering an effective amount of wild-type, or preferably a mutated form of, B. anthracis lethal factor (LF) or an immunogenic fragment thereof to the subject. A second method comprises administering an effective amount of a mutated LF protein or an immunogenic fragment of an LF protein and an effective amount of the B. anthracis protective antigen (PA) or an immunogenic fragment of the PA protein to the subject. A third method comprises administering a polynucleotide or nucleic acid comprising a sequence encoding a mutated B. anthracis LF protein or an immunogenic fragment of an LF protein to the subject. A fourth method comprises administering a polynucleotide which comprises a coding sequence for a mutated LF protein or an immunogenic fragment of an LF protein and a polynucleotide which comprises a coding sequence for the B. anthracis PA protein or an immunogenic fragment thereof to the subject. The present invention also relates to a protein or peptide based-immunogenic composition for preparing a vaccine which is capable of prophylactically protecting a subject against lethal effects of infection with B. anthracis or exposure to a toxic agent which is produced by B. anthracis. The protein or peptide based immunogenic composition comprises a purified or recombinant LF protein or immunogenic fragment thereof and a purified or recombinant PA protein or immunogenic fragment thereof. The present invention also relates to a nucleic acid-based immunogenic composition comprising a nucleic acid which comprises a sequence encoding the LF protein or an immunogenic fragment thereof and a polynucleotide which comprises a sequence encoding the PA protein or an immunogenic fragment thereof.
More Like This:
Inventors:
GALLOWAY DARRELL R (US)
MATECZUN ALFRED J (US)
MATECZUN ALFRED J (US)
Application Number:
PCT/US2000/034912
Publication Date:
January 10, 2002
Filing Date:
December 21, 2000
Export Citation:
Assignee:
UNIV OHIO STATE RES FOUND (US)
GALLOWAY DARRELL R (US)
MATECZUN ALFRED J (US)
GALLOWAY DARRELL R (US)
MATECZUN ALFRED J (US)
International Classes:
A61K39/07; C07K14/32; A61K39/00; (IPC1-7): C07H21/04; A61K48/00; A61K39/00; A61K39/07; A61K39/02; A61K39/385; C07K14/00; C07K16/00; C07K17/00; A01N63/00; C12N15/63
Foreign References:
| US5591631A | 1997-01-07 | |||
| US5677274A | 1997-10-14 |
Other References:
COHEN ET AL.: "Attenuated nontoxinogenic and nonencapsulated recombinant bacillus anthracis spore vaccines protect against anthrax", INFECTION AND IMMUNITY, vol. 68, no. 8, August 2000 (2000-08-01), pages 4549 - 4558, XP002942521
BARNARD ET AL.: "Vaccination against anthrax with attenuated recombinant strains of bacillus anthracis that produce protective antigen", INFECTION AND IMMUNITY, vol. 97, no. 2, February 1999 (1999-02-01), pages 562 - 567, XP002942520
LITTLE ET AL.: "Comparative efficacy of bacillus anthracis live spore vaccine and protective antigen vaccine against anthrax in the guinea pig", INFECTION AND IMMUNITY, vol. 52, no. 2, May 1986 (1986-05-01), pages 509 - 512, XP002942519
BRAGG ET AL.: "Nucleotide sequence and analysis of the lethal factor gene (lef) from bacillus anthracis", GENE, vol. 81, 1989, pages 45 - 54, XP002942518
BARNARD ET AL.: "Vaccination against anthrax with attenuated recombinant strains of bacillus anthracis that produce protective antigen", INFECTION AND IMMUNITY, vol. 97, no. 2, February 1999 (1999-02-01), pages 562 - 567, XP002942520
LITTLE ET AL.: "Comparative efficacy of bacillus anthracis live spore vaccine and protective antigen vaccine against anthrax in the guinea pig", INFECTION AND IMMUNITY, vol. 52, no. 2, May 1986 (1986-05-01), pages 509 - 512, XP002942519
BRAGG ET AL.: "Nucleotide sequence and analysis of the lethal factor gene (lef) from bacillus anthracis", GENE, vol. 81, 1989, pages 45 - 54, XP002942518
Download PDF: