METHODS FOR RECOVERING MICROORGANISM AND NUCLEIC ACID USING FINE PARTICLE AND KIT TO BE USED FOR THE METHODS

Title:

METHODS FOR RECOVERING MICROORGANISM AND NUCLEIC ACID USING FINE PARTICLE AND KIT TO BE USED FOR THE METHODS

Document Type and Number:

WIPO Patent Application WO/2006/123781

Kind Code:

A1

Abstract:

It is intended to provide methods for recovering a microorganism and a nucleic acid, which are simple and have a high recovery rate. The method for recovering a microorganism of the invention comprises the step of adsorbing a microorganism in which a sample is brought into contact with a fine particle and a microorganism in the sample is adsorbed to the fine particle and is characterized in that the particle diameter of the fine particle is 6 μm or less and the specific surface area thereof is 50 m²/g or less. The method for recovering a nucleic acid of the invention comprises the steps of adsorbing a microorganism in which a microorganism in a sample is adsorbed to a fine particle and eluting a nucleic acid in which a nucleic acid is eluted from the microorganism adsorbed to the fine particle and is characterized in that the step of adsorbing a microorganism is the method for recovering a microorganism of the invention. According to the recovery methods of the invention, a microorganism and a nucleic acid can be recovered simply and efficiently. The fine particle is preferably a magnetic silica particle.

Inventors:

HASHIGUCHI SATOSHI
HIRAI MITSUHARU
HOSOMI TOSHIYA

Application Number:

PCT/JP2006/310043

Publication Date:

November 23, 2006

Filing Date:

May 19, 2006

Export Citation:

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Assignee:

ARKRAY INC (JP)
HASHIGUCHI SATOSHI
HIRAI MITSUHARU
HOSOMI TOSHIYA

International Classes:

C12N15/09; C12N1/02; C12N7/02; C12N11/14

Foreign References:

JP2002165591A	2002-06-11
JPS6328392A	1988-02-06
JPS62269691A	1987-11-24
JP2003265164A	2003-09-24

Other References: