Title:
METHODS FOR SCREENING FOR AGENTS CAPABLE OF MODULATING T LYMPHOCYTE FUNCTION IN RESPONSE TO A HERPES SIMPLEX VIRUS-INFECTED CELL
Document Type and Number:
WIPO Patent Application WO2005049800
Kind Code:
A3
Abstract:
Disclosed are methods for sreening an agent for activity in modulating a T lymphocyte. The methods generally comprises contacting a HSV Us3-expressin cell with a T lymphocyte in the presence or absence of the agent and monitoring a physiological change associated with CTL function to determine whether the agent modulates CTL activity. In particular, the HSV Us3-expressing cell line can be a HSV-infected fibrolast and the physiological change associated with CTL function is a recduction or inhibition of the phosphorylation of HSP90 and/or a 50 kD CTL protein. Also disclosed are methods for blocking suppression of CTL activity against HSV-infected target cells, as well as methods for suppressing CTL activity against a target antigen.
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Inventors:
JEROME KEITH R (US)
SLOAN DEREK D (US)
SLOAN DEREK D (US)
Application Number:
PCT/US2004/038050
Publication Date:
October 20, 2005
Filing Date:
November 15, 2004
Export Citation:
Assignee:
HUTCHINSON FRED CANCER RES (US)
JEROME KEITH R (US)
SLOAN DEREK D (US)
JEROME KEITH R (US)
SLOAN DEREK D (US)
International Classes:
A61K38/16; A61K48/00; C07K14/035; C07K16/28; C12Q1/68; A61K38/095; C12N; (IPC1-7): C12Q1/68
Foreign References:
| US20030171279A1 | 2003-09-11 | |||
| US6218103B1 | 2001-04-17 |
Other References:
MUNGER J. ET AL: "The U(S)3 protein kinase blocks apoptosis induced by the d120 mutant of herpes simplex virus 1 at a premitochondrial stage.", JOURNAL OF VIROLOGY., vol. 75, no. 12, June 2001 (2001-06-01), pages 5491 - 5497, XP002990528
MUNGER J AND ROIZMAN B. ET AL: "The US3 protein kinase of herpes simplex virus 1 mediates the posttranslational modofication of BAD and prevents BAD-induced programmed cell death in the absence of other viral proteins.", PROC.NATL.ACAD.SCI.USA., vol. 98, no. 18, 28 August 2001 (2001-08-28), pages 10410 - 10415, XP002990529
GALVAN V. ET AL: "Bcl-2 blocks a caspase-dependent pathway of apoptosis activated by herpes simplex virus 1 infection in HEp-2 cells.", JOURNAL OF VIROLOGY., vol. 74, no. 4, February 2000 (2000-02-01), pages 1931 - 1938, XP002990530
MUNGER J AND ROIZMAN B. ET AL: "The US3 protein kinase of herpes simplex virus 1 mediates the posttranslational modofication of BAD and prevents BAD-induced programmed cell death in the absence of other viral proteins.", PROC.NATL.ACAD.SCI.USA., vol. 98, no. 18, 28 August 2001 (2001-08-28), pages 10410 - 10415, XP002990529
GALVAN V. ET AL: "Bcl-2 blocks a caspase-dependent pathway of apoptosis activated by herpes simplex virus 1 infection in HEp-2 cells.", JOURNAL OF VIROLOGY., vol. 74, no. 4, February 2000 (2000-02-01), pages 1931 - 1938, XP002990530
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