Title:
METHODS FOR SEQUENCING AND ANALYZING NUCLEIC ACIDS
Document Type and Number:
WIPO Patent Application WO/2018/235938
Kind Code:
A1
Abstract:
The present invention provides methods for correcting errors which occur in the digital assay of nucleic acids using molecular bar codes. More specifically, provided are: a method for identifying mispairing of molecular bar codes and index sequences in accordance with the detection frequency; a method for identifying molecular bar codes having base substitutions which have been categorized into the same cluster; and a method for identifying molecular bar codes having insertions or deletions using molecular bar codes including fixed bases and random bases.
Inventors:
SHIROGUCHI KATSUYUKI (JP)
Application Number:
PCT/JP2018/023778
Publication Date:
December 27, 2018
Filing Date:
June 22, 2018
Export Citation:
Assignee:
RIKEN (JP)
International Classes:
C12Q1/6869; C12Q1/6844
Domestic Patent References:
| WO2016002875A1 | 2016-01-07 |
Foreign References:
| JP2017503512A | 2017-02-02 | |||
| JP2017514487A | 2017-06-08 | |||
| US20150275289A1 | 2015-10-01 |
Other References:
SHIROGUCHI, KATSUYUKI: "Standardization of genome-wide absolute and accurate counting of RNA molecules", RESEARCH PERFORMANCE REPORT ON KAKENHI, no. 26640119, 3 June 2016 (2016-06-03)
MATSUMOTO, YUKI ET AL.: "Frame for digital RNA-seq: Accurate quantification of nucleic acid molecule by random barcode and its evaluation criteria (non-official translation)", ABSTRACTS OF COLLABORATIVE CONFERENCE OF THE 38TH ANNUAL CONFERENCE OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN AND THE 88TH CONFERENCE OF THE JAPANESE BIOCHEMICAL SOCIETY, 2015
MATSUMOTO, Y. ET AL.: "A framework for digital RNA sequencing (dRNA-seq) and its application for gene expression profiling of small number of immune-related cells", EUROPEAN JOURNAL OF IMMUNOLOGY, vol. 46, no. suppl. 1, 1203, 2016, pages 1162, XP055655719
SHIROGUCHI, KATSUYUKI: "Development of genome-side digital quantification method for non-coding RNA's wich has single-base identification and single-molecule level quantification", RESEARCH PERFORMANCE REPORT ON KAKENHI, no. 15K14425, 22 March 2018 (2018-03-22)
MATSUMOTO, YUKI ET AL.: "Frame for digital RNA-seq: Accurate quantification of nucleic acid molecule by random barcode and its evaluation criteria (non-official translation)", ABSTRACTS OF COLLABORATIVE CONFERENCE OF THE 38TH ANNUAL CONFERENCE OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN AND THE 88TH CONFERENCE OF THE JAPANESE BIOCHEMICAL SOCIETY, 2015
MATSUMOTO, Y. ET AL.: "A framework for digital RNA sequencing (dRNA-seq) and its application for gene expression profiling of small number of immune-related cells", EUROPEAN JOURNAL OF IMMUNOLOGY, vol. 46, no. suppl. 1, 1203, 2016, pages 1162, XP055655719
SHIROGUCHI, KATSUYUKI: "Development of genome-side digital quantification method for non-coding RNA's wich has single-base identification and single-molecule level quantification", RESEARCH PERFORMANCE REPORT ON KAKENHI, no. 15K14425, 22 March 2018 (2018-03-22)
Attorney, Agent or Firm:
OHNO Seiji et al. (JP)
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