METHODS AND SYSTEMS RELATED TO HIGHLY SENSITIVE, DIGITAL ASSAYS BY IMPROVED DELIVERY OF CAPTURE OBJECTS

WIPO Patent Application WO/2021/211754

A3

Methods and systems for capture object-based assays, including for determining a measure of the concentration of an analyte molecule or particle in a fluid sample, are described. The methods and systems may relate to high sensitivity detection of analytes, sometimes using assay conditions and sample handling that result in the capture and detection of a high percentage of the analyte molecules or particles in a fluid sample using relatively few capture objects. Apparatuses and methods for immobilizing capture objects with respect to assay sites, in some instances with unexpectedly high efficiencies are also described. Some such apparatuses involve the use of force fields and fluid meniscus forces, alone or in combination, to facilitate or improve capture object immobilization. Also described are techniques for utilizing a relatively high percentage of capture objects in an assay sample, such as by using disclosed sample washing techniques, imaging systems, and analysis procedures that can reduce capture object loss.

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DUFFY DAVID (US)
COLLIER NICHOLAS (GB)
KAN CHEUK (US)
TOBOS CARMEN (US)
RISSIN DAVID (US)
WIENER ALEXANDER (US)
MEYER RAY (US)
WARWICK CHRISTOPHER (GB)
MILLINGTON ROGER (GB)
SVANCARA DANIELLE (US)

PCT/US2021/027347

December 09, 2021

April 14, 2021

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QUANTERIX CORP (US)

B01L3/00; G01N1/40; G01N35/00; G01N35/08

WO2011109364A2

2011-09-09

US20090283407A1

2009-11-19

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POMIANEK, Michael, J. et al. (US)

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