Title:
METHODS TO PREPARE ANTIBODIES WHICH BIND TO POSTTRANSLATIONALLY MODIFIED AMINO ACIDS WITHIN PEPTIDES
Document Type and Number:
WIPO Patent Application WO1999065946
Kind Code:
A3
Abstract:
The invention relates to a process for preparing a spectrum of antibodies by use of a peptide library as an immunogenic substrate. Such antibody spectra are useful in that they may be used to identify, localise or measure amino acids within peptides involved in post-translational modifications and, consequently, may be used in screening for inhibitors of enzymes which are involved in such post-translational modifications. In particular the invention relates to preparing a spectrum of antibodies raised using a peptide library in which the majority of peptides contain a consensus sequence for a domain within which a serine or threonine is phosphorylated, where such a spectrum or part thereof is useful in screening, particularly in a high throughput manner, for inhibitors of serine or threonine kinase.
Inventors:
GARMAN ANDREW JOHN (GB)
Application Number:
PCT/GB1999/001795
Publication Date:
February 03, 2000
Filing Date:
June 08, 1999
Export Citation:
Assignee:
ZENECA LTD (GB)
GARMAN ANDREW JOHN (GB)
GARMAN ANDREW JOHN (GB)
International Classes:
C07K16/00; (IPC1-7): C07K16/00
Domestic Patent References:
| WO1989003430A1 | 1989-04-20 | |||
| WO1996031613A1 | 1996-10-10 |
Other References:
HEFFETZ D ET AL: "Generation and use of antibodies to phosphothreonine.", METHODS IN ENZYMOLOGY, (1991) 201 44-53., XP000857022
LEVINE L. ET AL: "Antibodies and radioimmunoassays for phosphoserine, phosphothreonine and phosphotyrosine. Serologic specificities and levels of the phosphoamino acids in cytoplasmic fractions of rat tissues.", JOURNAL OF IMMUNOLOGICAL METHODS, (1989) 124/2 (239-249)., XP002123393
LEVINE L. ET AL: "Antibodies and radioimmunoassays for phosphoserine, phosphothreonine and phosphotyrosine. Serologic specificities and levels of the phosphoamino acids in cytoplasmic fractions of rat tissues.", JOURNAL OF IMMUNOLOGICAL METHODS, (1989) 124/2 (239-249)., XP002123393
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