Title:
MICROFLUIDIC BIOMIMETIC FIBER FOR PRODUCING CELL CULTURED MEAT, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/007982
Kind Code:
A1
Abstract:
Provided are a microfluidic biomimetic fiber for producing cell cultured meat, a preparation method therefor and the use thereof. The microfluidic biomimetic fiber has a shell-core structure, wherein the shell is formed by means of crosslinking a cell-non-adhesive high polymer, and the inner core wrapped by the shell is a hydrogel solution mixed with seed cells. Inner phase fluid and outer phase fluid are respectively introduced into an inner phase channel and an outer phase channel of a microfluidic device, the two-phase fluid forms a stable laminar flow structure in a microfluidic device channel by means of adjusting the flow velocities of the inner phase and the outer phase, which is extruded from an outlet of the device to obtain biomimetic fiber. The method for constructing the biomimetic fiber on the basis of the microfluidic technique has the advantages of simple preparation, rapid prototyping and mild reaction. The prepared biomimetic fiber can be used for cell cultured meat production after culturing. The seed cells in the biomimetic fiber are directionally arranged, migrated, fused and grown in a fiber carrier, and therefore the differentiative capacity of the seed cells is significantly improved, the synthesis of muscle-related protein is increased, and the efficiency of cell cultured meat production is improved.
Inventors:
WANG JIE (CN)
ZHOU GUANGHONG (CN)
DING XI (CN)
ZHOU GUANGHONG (CN)
DING XI (CN)
Application Number:
PCT/CN2023/104613
Publication Date:
January 11, 2024
Filing Date:
June 30, 2023
Export Citation:
Assignee:
UNIV NANJING AGRICULTURAL (CN)
International Classes:
C12N5/077; A23J3/22; D01F1/10; D01F8/02; D01F8/18
Domestic Patent References:
| WO2022038240A2 | 2022-02-24 |
Foreign References:
| CN110643512A | 2020-01-03 | |||
| CN111500523A | 2020-08-07 |
Other References:
LI XIUFENG, YOU BAIHAO, SHUM HO CHEUNG, CHEN CHIA-HUNG: "Future foods: Design, fabrication and production through microfluidics", BIOMATERIALS, ELSEVIER, AMSTERDAM, NL, vol. 287, 1 August 2022 (2022-08-01), AMSTERDAM, NL , pages 121631, XP093126939, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2022.121631
HSIAO AMY Y., OKITSU TERU, ONOE HIROAKI, KIYOSAWA MAHIRO, TERAMAE HIROKI, IWANAGA SHINTAROH, KAZAMA TOMOHIKO, MATSUMOTO TARO, TAKE: "Smooth Muscle-Like Tissue Constructs with Circumferentially Oriented Cells Formed by the Cell Fiber Technology", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 10, no. 3, 3 March 2015 (2015-03-03), US , pages e0119010, XP093126943, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0119010
ONOE, H. ET AL.: "Metre-Long Cell-Laden Microfibres Exhibit Tissue Morphologies and Functions", NATURE MATERIALS, vol. 12, 30 June 2013 (2013-06-30), XP055963038, DOI: 10.1038/nmat3606
IKEDA KAZUHIRO, NAGATA SHOGO, OKITSU TERU, TAKEUCHI SHOJI: "Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 7, no. 1, US , XP093126944, ISSN: 2045-2322, DOI: 10.1038/s41598-017-03246-2
JUN YESL, KANG EDWARD, CHAE SUKYOUNG, LEE SANG-HOON: "Microfluidic spinning of micro- and nano-scale fibers for tissue engineering", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 14, no. 13, 1 January 2014 (2014-01-01), UK , pages 2145 - 2160, XP093126945, ISSN: 1473-0197, DOI: 10.1039/C3LC51414E
WANG GEN, JIA LUANLUAN, HAN FENGXUAN, WANG JIAYUAN, YU LI, YU YINGKANG, TURNBULL GARETH, GUO MINGYU, SHU WENMIAO, LI BIN: "Microfluidics-Based Fabrication of Cell-Laden Hydrogel Microfibers for Potential Applications in Tissue Engineering", MOLECULES, MDPI AG, CH, vol. 24, no. 8, CH , pages 1633, XP093126949, ISSN: 1420-3049, DOI: 10.3390/molecules24081633
HSIAO AMY Y., OKITSU TERU, ONOE HIROAKI, KIYOSAWA MAHIRO, TERAMAE HIROKI, IWANAGA SHINTAROH, KAZAMA TOMOHIKO, MATSUMOTO TARO, TAKE: "Smooth Muscle-Like Tissue Constructs with Circumferentially Oriented Cells Formed by the Cell Fiber Technology", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 10, no. 3, 3 March 2015 (2015-03-03), US , pages e0119010, XP093126943, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0119010
ONOE, H. ET AL.: "Metre-Long Cell-Laden Microfibres Exhibit Tissue Morphologies and Functions", NATURE MATERIALS, vol. 12, 30 June 2013 (2013-06-30), XP055963038, DOI: 10.1038/nmat3606
IKEDA KAZUHIRO, NAGATA SHOGO, OKITSU TERU, TAKEUCHI SHOJI: "Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 7, no. 1, US , XP093126944, ISSN: 2045-2322, DOI: 10.1038/s41598-017-03246-2
JUN YESL, KANG EDWARD, CHAE SUKYOUNG, LEE SANG-HOON: "Microfluidic spinning of micro- and nano-scale fibers for tissue engineering", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 14, no. 13, 1 January 2014 (2014-01-01), UK , pages 2145 - 2160, XP093126945, ISSN: 1473-0197, DOI: 10.1039/C3LC51414E
WANG GEN, JIA LUANLUAN, HAN FENGXUAN, WANG JIAYUAN, YU LI, YU YINGKANG, TURNBULL GARETH, GUO MINGYU, SHU WENMIAO, LI BIN: "Microfluidics-Based Fabrication of Cell-Laden Hydrogel Microfibers for Potential Applications in Tissue Engineering", MOLECULES, MDPI AG, CH, vol. 24, no. 8, CH , pages 1633, XP093126949, ISSN: 1420-3049, DOI: 10.3390/molecules24081633
Attorney, Agent or Firm:
NANJING SUGAO PATENT AND TRADEMARK FIRM (ORDINARY PARTNERSHIP) (CN)
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