Title:
MICROFLUIDIC DEVICE FOR DETECTING NUCLEIC ACIDS
Document Type and Number:
WIPO Patent Application WO/2022/019699
Kind Code:
A1
Abstract:
The present invention relates to a microfluidic device for detecting nucleic acids, the microfluidic device comprising: a chip main body; a sample chamber formed inside the chip main body and into which a sample is injected; a waste chamber spaced apart from the sample chamber and formed inside the chip main body; a connection conduit formed inside the chip main body to connect the sample chamber and the waste chamber to thereby form a flow path of the sample in the sample chamber, and having an inlet connected to the sample chamber and an outlet connected to the waste chamber; a nucleic acid detection membrane provided at the inlet of the connection conduit and having at least one micropore passing therethrough in the flow direction of the sample; and a probe linker formed on the surface of the nucleic acid detection membrane, wherein the probe linker is amplified through complementary binding with a target nucleic acid in the sample to detect the target nucleic acid. Accordingly, by installing the nucleic acid detection membrane at the inlet of the connection conduit to enable flow through the micropore of the nucleic acid detection membrane when flowing from the sample chamber to the connection conduit, the bubble generation phenomenon can be eliminated, thus enhancing the reproducibility of detection.
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Inventors:
SHIN SE-HYUN (KR)
Application Number:
PCT/KR2021/009538
Publication Date:
January 27, 2022
Filing Date:
July 23, 2021
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
B01L3/00; B01L7/02
Foreign References:
| KR20170110864A | 2017-10-12 | |||
| KR20160052400A | 2016-05-12 | |||
| KR20130135111A | 2013-12-10 | |||
| KR101799192B1 | 2017-11-17 |
Other References:
CHANGCHUN LIU, ERAN GEVA, MICHAEL MAUK, XIANBO QIU, WILLIAM R. ABRAMS, DANIEL MALAMUD, KELLY CURTIS, S. MICHELE OWEN, HAIM H. BAU: "An isothermal amplification reactor with an integrated isolation membrane for point-of-care detection of infectious diseases", ANALYST, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 136, no. 10, 1 January 2011 (2011-01-01), UK , pages 2069, XP055753357, ISSN: 0003-2654, DOI: 10.1039/c1an00007a
"Clinical Applications of Mass Spectrometry", vol. 1256, 1 January 2015, HUMANA PRESS , Totowa, NJ , ISBN: 9781607614593, ISSN: 10643745, article MICHAEL G. MAUK, CHANGCHUN LIU, MOHAMED SADIK, HAIM H. BAU: "Microfluidic Devices for Nucleic Acid (NA) Isolation, Isothermal NA Amplification, and Real-Time Detection", pages: 15 - 40, XP055195542, DOI: 10.1007/978-1-4939-2172-0_2
C. LARSSON ET AL., NAT. METHODS, vol. 1, 2004, pages 227
HO YEON LEE ET AL.: "DhITACT: DNA Hydrogel Formation by Isothermal Amplification of Complementary Target in Fluidic Channels", ADVANCED MATERIALS, vol. 27, 17 June 2015 (2015-06-17), pages 3513 - 3517, XP055424328, DOI: 10.1002/adma.201500414
See also references of EP 4186594A4
"Clinical Applications of Mass Spectrometry", vol. 1256, 1 January 2015, HUMANA PRESS , Totowa, NJ , ISBN: 9781607614593, ISSN: 10643745, article MICHAEL G. MAUK, CHANGCHUN LIU, MOHAMED SADIK, HAIM H. BAU: "Microfluidic Devices for Nucleic Acid (NA) Isolation, Isothermal NA Amplification, and Real-Time Detection", pages: 15 - 40, XP055195542, DOI: 10.1007/978-1-4939-2172-0_2
C. LARSSON ET AL., NAT. METHODS, vol. 1, 2004, pages 227
HO YEON LEE ET AL.: "DhITACT: DNA Hydrogel Formation by Isothermal Amplification of Complementary Target in Fluidic Channels", ADVANCED MATERIALS, vol. 27, 17 June 2015 (2015-06-17), pages 3513 - 3517, XP055424328, DOI: 10.1002/adma.201500414
See also references of EP 4186594A4
Attorney, Agent or Firm:
CHOI, Hoon-Sik (KR)
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