Title:
MICROFLUIDIC DEVICES AND SYSTEMS
Document Type and Number:
WIPO Patent Application WO/2015/015199
Kind Code:
A3
Abstract:
We describe a method of label-free cell and/or particle sorting in a microfluidic device, the method comprising; providing a stream of aqueous droplets in oil in a channel of said microfluidic device, wherein at least some of said droplets include cells and/or particles illuminating said stream from a first direction; detecting, for example, scattered light from cells and/or particles within said aqueous droplets in a second direction; determining, for example, a number of said cells and/or particles in each droplet from the scattered light; and sorting said aqueous droplets into one of a plurality of differentiated streams responsive to said determined number. The method further comprises modifying a refractive index of said oil to more closely match a refractive index of said aqueous droplets to reduce light scattered from boundaries of said droplets. Optionally a volume of the droplets is also controlled. In embodiments the oil is fluorous oil. We describe examples of preferred refractive index modifying compounds.
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Inventors:
SMITH CLIVE A (GB)
LI XIN (GB)
ENESA KARINE (GB)
LIU XIN (GB)
CRAIG FRANK F (GB)
WHYTE GRAEME (DE)
LI XIN (GB)
ENESA KARINE (GB)
LIU XIN (GB)
CRAIG FRANK F (GB)
WHYTE GRAEME (DE)
Application Number:
PCT/GB2014/052332
Publication Date:
June 04, 2015
Filing Date:
July 30, 2014
Export Citation:
Assignee:
SPHERE FLUIDICS LTD (GB)
International Classes:
B01L3/00
Foreign References:
| US20080053205A1 | 2008-03-06 |
Other References:
DANIEL R GOSSETT ET AL: "Label-free cell separation and sorting in microfluidic systems", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, SPRINGER, BERLIN, DE, vol. 397, no. 8, 25 April 2010 (2010-04-25), pages 3249 - 3267, XP019839258, ISSN: 1618-2650
Attorney, Agent or Firm:
MARKS & CLERK LLP (Cambridge, Cambridgeshire CB2 1LA, GB)
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