Title:
MILK-DERIVED EXOSOME AND EXTRACTION METHOD
Document Type and Number:
WIPO Patent Application WO/2024/001457
Kind Code:
A1
Abstract:
Provided is an extraction method for a milk-derived exosome, which successively carries out the following steps: (1) dividing milk raw materials, centrifuging same, and collecting a clear liquid; (2) centrifuging the clear liquid obtained in the step (1), and collecting a middle-layer clear liquid; (3) centrifuging the middle-layer clear liquid obtained in the step (2), collecting a clear liquid, uniformly mixing same with a PBS buffer solution and an exosome extraction reagent, and incubating same; (4) centrifuging the product in the step (3), keeping a precipitate, and resuspending same in a PBS buffer solution; and (5) centrifuging the product in the step (4), and collecting the supernatant as a product.
Inventors:
CHEN LIJUN (CN)
LI YING (CN)
CHEN JINGYAO (CN)
QIAO WEICANG (CN)
ZHANG MINGHUI (CN)
ZHAO JUNYING (CN)
YANG BAOYU (CN)
LI YING (CN)
CHEN JINGYAO (CN)
QIAO WEICANG (CN)
ZHANG MINGHUI (CN)
ZHAO JUNYING (CN)
YANG BAOYU (CN)
Application Number:
PCT/CN2023/089769
Publication Date:
January 04, 2024
Filing Date:
April 21, 2023
Export Citation:
Assignee:
BEIJING SANYUAN FOODS CO LTD (CN)
International Classes:
C12N5/071
Foreign References:
| CN114891723A | 2022-08-12 | |||
| CN109468265A | 2019-03-15 | |||
| US10166259B1 | 2019-01-01 | |||
| US20170296626A1 | 2017-10-19 |
Other References:
SHIJIE FAN, MA LU; BU DENGPAN: "Comparison of Jersey Milk Derived Exosomes Extracted by Differential Ultracentrifugation Method and Kit Extraction Method ", CHINESE JOURNAL OF ANIMAL NUTRITION, vol. 32, no. 11, 8 July 2020 (2020-07-08), pages 5441 - 5447, XP093125134
YING LI, JINGYAO CHEN, JUNYING ZHAO, XIAOWEN YU, YIHAN YANG, JUFENG HU, TIEMIN JIANG, LIJUN CHEN: "Research Progress in Milk-derived Exosomes", SCIENCE AND TECHNOLOGY OF FOOD INDUSTRY, GAI KAN BIANJIBU , BEIJING, CN, vol. 43, no. 22, 17 March 2022 (2022-03-17), CN , pages 406 - 413, XP093125138, ISSN: 1002-0306, DOI: 10.13386/j.issn1002-0306.2021090317
YING LI, JINGYAO CHEN, JUNYING ZHAO, XIAOWEN YU, YIHAN YANG, JUFENG HU, TIEMIN JIANG, LIJUN CHEN: "Research Progress in Milk-derived Exosomes", SCIENCE AND TECHNOLOGY OF FOOD INDUSTRY, GAI KAN BIANJIBU , BEIJING, CN, vol. 43, no. 22, 17 March 2022 (2022-03-17), CN , pages 406 - 413, XP093125138, ISSN: 1002-0306, DOI: 10.13386/j.issn1002-0306.2021090317
Attorney, Agent or Firm:
BEIJING YUEHE INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)
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