Title:
MODIFIED DAAO ENZYME AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/115256
Kind Code:
A1
Abstract:
The present invention relates to a modified D-amino acid oxidase (DAAO). Specifically, the modified DAAO has activity of catalyzing glufosinate D to be oxidized to PPO. In addition, the modified DAAO has improved activity of catalyzing the glufosinate D to be oxidized to PPO and/or improved stability compared with SEQ ID NO:4. The present invention further relates to a polynucleotide encoding the modified DAAO, a vector and host cell expressing the modified DAAO, and a method for producing L-glufosinate using the modified DAAO and the host cell.
Inventors:
XIE XINKAI (CN)
XU WEI (CN)
FAN JUNYING (CN)
XU WEI (CN)
FAN JUNYING (CN)
Application Number:
PCT/CN2020/134540
Publication Date:
June 17, 2021
Filing Date:
December 08, 2020
Export Citation:
Assignee:
SICHUAN LIER BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N15/53; C12Q1/26; C12P41/00
Domestic Patent References:
| WO2020051188A1 | 2020-03-12 |
Foreign References:
| CN111019916A | 2020-04-17 | |||
| CN109072261A | 2018-12-21 | |||
| CN109576236A | 2019-04-05 | |||
| CN1639344A | 2005-07-13 | |||
| US20190185889A1 | 2019-06-20 | |||
| CN108690854A | 2018-10-23 | |||
| CN105567780A | 2016-05-11 | |||
| US20180030487A1 | 2018-02-01 | |||
| CN107502647A | 2017-12-22 | |||
| CN109576236A | 2019-04-05 | |||
| CN109609582A | 2019-04-12 | |||
| US7939709B2 | 2011-05-10 | |||
| US9834802B2 | 2017-12-05 |
Other References:
HAWKES, T. ET AL.: "D-glufosinate as a Male Sterility Agent for Hybrid Seed Production", PLANT BIOTECHNOLOGY JOURNAL, vol. 9, no. 3, 30 April 2011 (2011-04-30), pages 301 - 314, XP055049757, DOI: 10.1111/j.1467-7652.2010.00549.x
SACCHI, S. ET AL.: "Engineering the Substrate Specificity of D-Amino-acid Oxidase", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 30, 26 July 2002 (2002-07-26), pages 27510 - 27516, XP002295815, DOI: 10.1074/jbc.M203946200
BOSELLI, A. ET AL.: "Investigating the Role of Active Site Residues of Rhodotorula Gracilis D-Amino Acid Oxidase on Its Substrate Specificity", BIOCHIMIE, vol. 89, 27 November 2006 (2006-11-27), pages 360 - 368, XP005934619, DOI: 10.1016/j.biochi.2006.10.017
ZHAO, RANRAN ET AL.: "Research Progress on the Crystal Structure of D-amino Acid Oxidase", BIOTECHNOLOGY BULLETIN, no. 12, 25 December 2013 (2013-12-25), pages 27 - 35, XP055822073
TIM HAWKS ET AL.: "D-glufosinate as a male sterility agent for hybrid seed production", PLANT BIOTECHNOLOGY JOURNAL, vol. 9, 2011, pages 301 - 314, XP055049757, DOI: 10.1111/j.1467-7652.2010.00549.x
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular Biology", 1995, JOHN WILEY & SONS
DAVIS ET AL., BASIC METHODS IN MOLECULAR BIOLOGY, 1986
NUCLEIC ACIDS RESEARCH, vol. 32, no. 14, 2004, pages e115
See also references of EP 4074837A4
SACCHI, S. ET AL.: "Engineering the Substrate Specificity of D-Amino-acid Oxidase", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 30, 26 July 2002 (2002-07-26), pages 27510 - 27516, XP002295815, DOI: 10.1074/jbc.M203946200
BOSELLI, A. ET AL.: "Investigating the Role of Active Site Residues of Rhodotorula Gracilis D-Amino Acid Oxidase on Its Substrate Specificity", BIOCHIMIE, vol. 89, 27 November 2006 (2006-11-27), pages 360 - 368, XP005934619, DOI: 10.1016/j.biochi.2006.10.017
ZHAO, RANRAN ET AL.: "Research Progress on the Crystal Structure of D-amino Acid Oxidase", BIOTECHNOLOGY BULLETIN, no. 12, 25 December 2013 (2013-12-25), pages 27 - 35, XP055822073
TIM HAWKS ET AL.: "D-glufosinate as a male sterility agent for hybrid seed production", PLANT BIOTECHNOLOGY JOURNAL, vol. 9, 2011, pages 301 - 314, XP055049757, DOI: 10.1111/j.1467-7652.2010.00549.x
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular Biology", 1995, JOHN WILEY & SONS
DAVIS ET AL., BASIC METHODS IN MOLECULAR BIOLOGY, 1986
NUCLEIC ACIDS RESEARCH, vol. 32, no. 14, 2004, pages e115
See also references of EP 4074837A4
Attorney, Agent or Firm:
NTD UNIVATION INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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