Title:
MODIFIED ESTERASE
Document Type and Number:
WIPO Patent Application WO/2024/043203
Kind Code:
A1
Abstract:
The present invention provides any of modified esterases of (i) to (iii) below. (i) A modified esterase including an amino acid sequence in which at least one amino acid at a site selected from the group consisting of (1) to (5) below is substituted with another amino acid in an amino acid sequence of SEQ ID NO: 1, 7, or 35: (1) S12, (2) R25, (3) S220, (4) A313, (5) S315. (ii) A modified esterase in which one or more amino acids (provided that the amino acid at the substitution site in (i) is excluded) are further substituted or deleted in the modified esterase of (i), or one or more amino acids are added or inserted into the modified esterase of (i) and in which temperature stability and/or resistance to organic solvents is improved than those of an esterase of an amino acid sequence of SEQ ID NO: 1, 7, or 35. (iii) A modified esterase in which an amino acid other than the amino acid substituted in (i) is further substituted with another amino acid in the modified esterase of (i), wherein the modified esterase has identity of 70% or more with the modified esterase of (i), and temperature stability and/or resistance to organic solvents is improved than those of an esterase of an amino acid sequence of SEQ ID NO: 1, 7, or 35.
Inventors:
ISHIHARA SATORU (JP)
Application Number:
PCT/JP2023/029951
Publication Date:
February 29, 2024
Filing Date:
August 21, 2023
Export Citation:
Assignee:
AMANO ENZYME INC (JP)
International Classes:
C12N15/55; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/16; C12P7/40
Domestic Patent References:
| WO2020116331A1 | 2020-06-11 |
Other References:
MARCUS SCHÜTTE ; SUSANNE FETZNER: "EstA from Arthrobacter nitroguajacolicus Rü61a, a Thermo and Solvent-Tolerant Carboxylesterase Related to Class C β-Lactamases", CURRENT MICROBIOLOGY, SPRINGER-VERLAG, NE, vol. 54, no. 3, 8 February 2007 (2007-02-08), Ne , pages 230 - 236, XP019490105, ISSN: 1432-0991, DOI: 10.1007/s00284-006-0438-2
Attorney, Agent or Firm:
KAMADA, Mitsunori et al. (JP)
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