Title:
MODIFIED β-GALACTOSIDASE
Document Type and Number:
WIPO Patent Application WO/2015/046408
Kind Code:
A1
Abstract:
The purpose of the present invention is to further increase the usefulness of β-galactosidase derived from Bacillus circulans, making possible use thereof in new applications. Provided is a β-galactosidase comprising an amino acid sequence represented by any of Seq. ID. No. 1 to 4, or an amino acid sequence having 90% or more homology to an amino acid sequence represented by any of Seq. ID. No. 1 to 4, wherein one or more amino acids selected from the 182nd proline, the 187th tyrosine, the 188th serine, the 405th tryptophan, the 406th alanine, the 407th glutamine, the 449th tyrosine, the 483rd threonine, the 512th serine, the 531st serine, the 533rd serine, the 534th serine, the 550th asparagine, the 551st glutamine, the 593rd tryptophan, the 598th tyrosine, the 602nd proline, the 604th proline, the 609th tyrosine, the 612th lysine, and the 615th tyrosine, or an amino acid corresponding thereto, is substituted by another amino acid.
Inventors:
NAKAMURA GENICHI (JP)
KURITANI YUKO (JP)
ISHIHARA SATORU (JP)
TAKAHASHI TETSUYA (JP)
KURITANI YUKO (JP)
ISHIHARA SATORU (JP)
TAKAHASHI TETSUYA (JP)
Application Number:
PCT/JP2014/075575
Publication Date:
April 02, 2015
Filing Date:
September 26, 2014
Export Citation:
Assignee:
AMANO ENZYME INC (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/38; C12P19/14
Domestic Patent References:
| WO2010140435A1 | 2010-12-09 | |||
| WO2010098561A2 | 2010-09-02 | |||
| WO2010140435A1 | 2010-12-09 | |||
| WO2010098561A2 | 2010-09-02 |
Foreign References:
| JPH09313177A | 1997-12-09 | |||
| JPH1118763A | 1999-01-26 | |||
| JPH1118763A | 1999-01-26 |
Other References:
SONG J. ET AL.: "The discoidin domain of Bacillus circulans beta-galactosidase plays an essential role in repressing galactooligosaccharide production.", BIOSCI BIOTECHNOL BIOCHEM., vol. 77, no. 1, January 2013 (2013-01-01), pages 73 - 79, XP055331755
DATABASE GENBANK [online] 28 June 2011 (2011-06-28), SONG J. ET AL.: "Definition: Bacillus circulans bga gene for beta galactosidase", XP055331756, retrieved from NCBI Database accession no. AB605256
SONG J. ET AL.: "Cloning and expression of a beta-galactosidase gene of Bacillus circulans.", BIOSCI BIOTECHNOL BIOCHEM., vol. 75, no. 6, 2011, pages 1194 - 1197, XP055331757
LI C. ET AL.: "Characterization of a galactosynthase derived from Bacillus circulans beta-galactosidase: facile synthesis of D-lacto- and D-galacto-N-bioside.", CHEMBIOCHEM., vol. 15, no. 4, March 2014 (2014-03-01), pages 522 - 526, XP055331758
HENZE M. ET AL.: "Rational design of a glycosynthase by the crystal structure of beta- galactosidase from Bacillus circulans (BgaC) and its use for the synthesis of N- acetyllactosamine type 1 glycan structures.", J BIOTECHNOL., vol. 191, July 2014 (2014-07-01), pages 78 - 85, XP029095952
URASHIMA ET AL., KAGAKU TO SEIBUTSU, vol. 50, 2012, pages 7
SONG ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 77, 2013, pages 73 - 79
"Molecular Cloning", COLD SPRING HARBOR LABORATORY PRESS, NEW YORK
FREDERICK M. AUSUBEL ET AL.: "Current protocols in molecular biology", 1987
"Molecular Cloning", vol. 1.84, COLD SPRING HARBOR LABORATORY PRESS
J. MOL. BIOL., vol. 53, 1970, pages 159
J. MOL. BIOL., vol. 166, 1983, pages 557
GENE, vol. 96, 1990, pages 23
CHUNG ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 86, 1989, pages 2172
POTTER, H. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 81, 1984, pages 7161 - 7165
FELGNER, P.L. ET AL., PROC. NATL. ACAD. SCI. U.S.A, vol. 84, 1984, pages 7413 - 7417
See also references of EP 3064581A4
DATABASE GENBANK [online] 28 June 2011 (2011-06-28), SONG J. ET AL.: "Definition: Bacillus circulans bga gene for beta galactosidase", XP055331756, retrieved from NCBI Database accession no. AB605256
SONG J. ET AL.: "Cloning and expression of a beta-galactosidase gene of Bacillus circulans.", BIOSCI BIOTECHNOL BIOCHEM., vol. 75, no. 6, 2011, pages 1194 - 1197, XP055331757
LI C. ET AL.: "Characterization of a galactosynthase derived from Bacillus circulans beta-galactosidase: facile synthesis of D-lacto- and D-galacto-N-bioside.", CHEMBIOCHEM., vol. 15, no. 4, March 2014 (2014-03-01), pages 522 - 526, XP055331758
HENZE M. ET AL.: "Rational design of a glycosynthase by the crystal structure of beta- galactosidase from Bacillus circulans (BgaC) and its use for the synthesis of N- acetyllactosamine type 1 glycan structures.", J BIOTECHNOL., vol. 191, July 2014 (2014-07-01), pages 78 - 85, XP029095952
URASHIMA ET AL., KAGAKU TO SEIBUTSU, vol. 50, 2012, pages 7
SONG ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 77, 2013, pages 73 - 79
"Molecular Cloning", COLD SPRING HARBOR LABORATORY PRESS, NEW YORK
FREDERICK M. AUSUBEL ET AL.: "Current protocols in molecular biology", 1987
"Molecular Cloning", vol. 1.84, COLD SPRING HARBOR LABORATORY PRESS
J. MOL. BIOL., vol. 53, 1970, pages 159
J. MOL. BIOL., vol. 166, 1983, pages 557
GENE, vol. 96, 1990, pages 23
CHUNG ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 86, 1989, pages 2172
POTTER, H. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 81, 1984, pages 7161 - 7165
FELGNER, P.L. ET AL., PROC. NATL. ACAD. SCI. U.S.A, vol. 84, 1984, pages 7413 - 7417
See also references of EP 3064581A4
Attorney, Agent or Firm:
HAGINO Mikiharu (JP)
Kanji Hagino (JP)
Kanji Hagino (JP)
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