Title:
MODIFIED HELICASES
Document Type and Number:
WIPO Patent Application WO/2015/055981
Kind Code:
A3
Abstract:
The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
Inventors:
BRUCE MARK (GB)
HERON ANDREW JOHN (GB)
MOYSEY RUTH (GB)
SOEROES SZABOLCS (GB)
WALLACE JAYNE ELIZABETH (GB)
WHITE JAMES (GB)
HERON ANDREW JOHN (GB)
MOYSEY RUTH (GB)
SOEROES SZABOLCS (GB)
WALLACE JAYNE ELIZABETH (GB)
WHITE JAMES (GB)
Application Number:
PCT/GB2014/052736
Publication Date:
June 11, 2015
Filing Date:
September 10, 2014
Export Citation:
Assignee:
OXFORD NANOPORE TECH LTD (GB)
International Classes:
C12Q1/68
Domestic Patent References:
| WO2013098562A2 | 2013-07-04 | |||
| WO2013057495A2 | 2013-04-25 | |||
| WO2013098561A1 | 2013-07-04 |
Other References:
RA CHOI ET AL: "Uniprot:K7NRI8", 6 February 2013 (2013-02-06), XP055156165, Retrieved from the Internet
RA PETROV ET AL: "Uniprot:Q7Y5C3", 1 October 2003 (2003-10-01), XP055156177, Retrieved from the Internet
SKURNIK M RA ET AL: "Uniprot:I7J3V8", 3 October 2012 (2012-10-03), XP055156392, Retrieved from the Internet
MARTIN R. SINGLETON ET AL: "Structure and Mechanism of Helicases and Nucleic Acid Translocases", ANNUAL REVIEW OF BIOCHEMISTRY, vol. 76, no. 1, 7 June 2007 (2007-06-07), pages 23 - 50, XP055056968, ISSN: 0066-4154, DOI: 10.1146/annurev.biochem.76.052305.115300
XIAOPING HE ET AL: "The T4 Phage SF1B Helicase Dda Is Structurally Optimized to Perform DNA Strand Separation", STRUCTURE, CURRENT BIOLOGY LTD., PHILADELPHIA, PA, US, vol. 20, no. 7, 21 April 2012 (2012-04-21), pages 1189 - 1200, XP028399006, ISSN: 0969-2126, [retrieved on 20120510], DOI: 10.1016/J.STR.2012.04.013
A. K. KAR ET AL: "Defining the Structure-Function Relationships of Bluetongue Virus Helicase Protein VP6", JOURNAL OF VIROLOGY, vol. 77, no. 21, 13 October 2003 (2003-10-13), pages 11347 - 11356, XP055156648, ISSN: 0022-538X, DOI: 10.1128/JVI.77.21.11347-11356.2003
RA PETROV ET AL: "Uniprot:Q7Y5C3", 1 October 2003 (2003-10-01), XP055156177, Retrieved from the Internet
SKURNIK M RA ET AL: "Uniprot:I7J3V8", 3 October 2012 (2012-10-03), XP055156392, Retrieved from the Internet
MARTIN R. SINGLETON ET AL: "Structure and Mechanism of Helicases and Nucleic Acid Translocases", ANNUAL REVIEW OF BIOCHEMISTRY, vol. 76, no. 1, 7 June 2007 (2007-06-07), pages 23 - 50, XP055056968, ISSN: 0066-4154, DOI: 10.1146/annurev.biochem.76.052305.115300
XIAOPING HE ET AL: "The T4 Phage SF1B Helicase Dda Is Structurally Optimized to Perform DNA Strand Separation", STRUCTURE, CURRENT BIOLOGY LTD., PHILADELPHIA, PA, US, vol. 20, no. 7, 21 April 2012 (2012-04-21), pages 1189 - 1200, XP028399006, ISSN: 0969-2126, [retrieved on 20120510], DOI: 10.1016/J.STR.2012.04.013
A. K. KAR ET AL: "Defining the Structure-Function Relationships of Bluetongue Virus Helicase Protein VP6", JOURNAL OF VIROLOGY, vol. 77, no. 21, 13 October 2003 (2003-10-13), pages 11347 - 11356, XP055156648, ISSN: 0022-538X, DOI: 10.1128/JVI.77.21.11347-11356.2003
Attorney, Agent or Firm:
CHAPMAN, Lee, Phillip (14 South SquareGray's Inn,London, Greater London WC1R 5JJ, GB)
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