Title:
MODIFIED ISOPRENE SYNTHASE
Document Type and Number:
WIPO Patent Application WO/2015/076392
Kind Code:
A1
Abstract:
Provided are an enzyme that is useful for establishing an excellent isoprene monomer-producing system, etc. Specifically, provided are: a modified isoprene synthase that has a mutation at a definite amino acid residue in one of the following amino acid sequences (a) to (c),
(a) an amino acid sequence represented by SEQ ID NO:4,
(b) an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO:4 by substitution, deletion, insertion or addition of one to several amino acids, and
(c) an amino acid sequence having 90% or more identity to the amino acid sequence represented by SEQ ID NO:4,
and has an isoprene-synthesizing activity; a polynucleotide that encodes the modified isoprene synthase; an expression vector that contains the polynucleotide; a host cell that contains an expression unit containing the polynucleotide; a method for producing an isoprene monomer, said method comprising synthesizing the isoprene monomer from dimethylallyl diphosphate in the presence of the modified isoprene synthase; etc.
Inventors:
NAKATA KUNIO (JP)
TAJIMA YOSHINORI (JP)
TAGAMI UNO (JP)
OKU TAKASHI (JP)
KASHIMA YASUHIRO (JP)
TAJIMA YOSHINORI (JP)
TAGAMI UNO (JP)
OKU TAKASHI (JP)
KASHIMA YASUHIRO (JP)
Application Number:
PCT/JP2014/080980
Publication Date:
May 28, 2015
Filing Date:
November 21, 2014
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C12N15/09; C08F36/08; C12N1/19; C12N1/21; C12N9/88; C12P5/02
Domestic Patent References:
| WO2013179722A1 | 2013-12-05 | |||
| WO2013166320A1 | 2013-11-07 | |||
| WO2013016591A1 | 2013-01-31 | |||
| WO2009076676A2 | 2009-06-18 | |||
| WO2010031062A1 | 2010-03-18 | |||
| WO2010027022A1 | 2010-03-11 | |||
| WO2007037460A1 | 2007-04-05 |
Foreign References:
| JP2012516677A | 2012-07-26 | |||
| JP2011505841A | 2011-03-03 | |||
| JP2011518564A | 2011-06-30 | |||
| US20110076743A1 | 2011-03-31 | |||
| US20130045891A1 | 2013-02-21 | |||
| US20130295632A1 | 2013-11-07 | |||
| US20130330709A1 | 2013-12-12 | |||
| US20130330796A1 | 2013-12-12 | |||
| US20110045563A1 | 2011-02-24 | |||
| US20110039323A1 | 2011-02-17 | |||
| EP0952221A2 | 1999-10-27 | |||
| JP2005058827A | 2005-03-10 |
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SAMBROOK, J. ET AL.: "Molecular Cloning: Laboratory Manuals", 1989, COLD SPRING HARBOR LABORATORY PRESS
CROTEASU R ET AL., J. BIOL. CHEM., vol. 261, no. 29, 1986, pages 13438 - 45
MONSON R.K ET AL., PLANT PHYSIOL., vol. 98, 1992, pages 1175 - 1180
PLANT PHYSIOL., vol. 101, 1993, pages 435 - 440
PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873
PEARSON, METHODS ENZYMOL., vol. 183, 1990, pages 63
J MOL BIOL, vol. 48, 1970, pages 443 - 453
BIOTECHNOL. BIOENG., vol. 98, no. 2, 27 March 2007 (2007-03-27), pages 340 - 348
J. P. SCHNITZLER ET AL., PLANTA, vol. 222, 2005, pages 777
M. MATSUMURA ET AL., NATURE, vol. 342, 1989, pages 291 - 293
GM. SILVER ET AL., J. BIOL. CHEM., vol. 270, 1995, pages 13010
M. J. ZVELEBIL ET AL., J. MOL. BIOL., vol. 195, 1987, pages 57
S. HENIKOFF ET AL., PROC. NATL. ACAD. USA, vol. 89, 1992, pages 10915
A. BACHMAIR ET AL., SCIENCE, vol. 234, 1986, pages 179 - 186
J. W. TOBIAS ET AL., SCIENCE, vol. 254, 1991, pages 1374 - 1377
DEBOER ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 80, 1983, pages 21 - 25
WU ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 75, 1978, pages 442 - 5446
APPL. MICROBIOL. BIOTECHNOL., vol. 90, 2011, pages 1915 - 1922
SCIENCE, vol. 277, 1997, pages 1453 - 1474
MOL GEN GENET., vol. 183, no. 2, 1981, pages 380 - 7
PROC. NATL. ACAD. SCI. USA, vol. 97, no. 12, 2000, pages 6640 - 6645
SAMBROOK, J. ET AL.: "Molecular Cloning: Laboratory Manuals", 1989, COLD SPRING HARBOR LABORATORY PRESS
CROTEASU R ET AL., J. BIOL. CHEM., vol. 261, no. 29, 1986, pages 13438 - 45
Attorney, Agent or Firm:
SAKAI, Hiroaki et al. (JP)
Hiroaki Sakai (JP)
Hiroaki Sakai (JP)
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