Title:
MODIFIED KLENOW FRAGMENT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/093434
Kind Code:
A1
Abstract:
Provided are a modified Klenow fragment and an application thereof; specifically provided is a modified Klenow fragment, wherein at least one position or functionally equivalent position among F762, A842, I709 and P603 in the amino acid sequence of the modified Klenow fragment contains at least one amino acid substitution mutation. The modified Klenow fragment has higher DNA polymerase activity than a wild-type Klenow fragment and can be applied to sequencing.
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Inventors:
CHEN QINGBIN (CN)
CHEN WEIYUE (CN)
LI HONGDAN (CN)
LIN XIA (CN)
WANG WEN (CN)
LUO WEIWEI (CN)
SUN LEI (CN)
CHEN WEIYUE (CN)
LI HONGDAN (CN)
LIN XIA (CN)
WANG WEN (CN)
LUO WEIWEI (CN)
SUN LEI (CN)
Application Number:
PCT/CN2020/114356
Publication Date:
May 20, 2021
Filing Date:
September 10, 2020
Export Citation:
Assignee:
GENEMIND BIOSCIENCES CO LTD (CN)
International Classes:
C12N9/12; C12N1/21; C12N15/31; C12N15/54; C12N15/64; C12Q1/686; C12Q1/6869
Domestic Patent References:
| WO2006037064A2 | 2006-04-06 | |||
| WO2006037064A2 | 2006-04-06 | |||
| WO2012097318A2 | 2012-07-19 | |||
| WO2012097318A2 | 2012-07-19 |
Foreign References:
| CN1170439A | 1998-01-14 | |||
| CN1170439A | 1998-01-14 | |||
| CN111534500A | 2020-08-14 | |||
| CN103492559A | 2014-01-01 | |||
| CN2018118259W | 2018-11-29 |
Other References:
DATABASE PROTEIN 21 January 2021 (2021-01-21), ANONYMOUS: "MULTISPECIES: DNA polymerase I [Enterobacteriaceae]", XP055813466, retrieved from NCBI Database accession no. WP_152028965
DATABASE PROTEIN 21 January 2021 (2021-01-21), ANONYMOUS: "MULTISPECIES: DNA polymerase I [Enterobacteriaceae]", XP055813466, retrieved from NCBI Database accession no. WP_152028965
M. ASTATKE, NG K., GRINDLEY N. D. F., JOYCE C. M.: "A single side chain prevents Escherichia coli DNA polymerase I (Klenow fragment) from incorporating ribonucleotides", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 95, no. 7, 31 March 1998 (1998-03-31), pages 3402 - 3407, XP055242309, ISSN: 0027-8424, DOI: 10.1073/pnas.95.7.3402
M. ASTATKE, NG K., GRINDLEY N. D. F., JOYCE C. M.: "A single side chain prevents Escherichia coli DNA polymerase I (Klenow fragment) from incorporating ribonucleotides", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 95, no. 7, 31 March 1998 (1998-03-31), pages 3402 - 3407, XP055242309, ISSN: 0027-8424, DOI: 10.1073/pnas.95.7.3402
DATABASE PROTEIN 1 June 2019 (2019-06-01), ANONYMOUS: "DNA polymerase I [Escherichia coli]", XP055813469, retrieved from NCBI Database accession no. WP_075861667
DATABASE PROTEIN 1 June 2019 (2019-06-01), ANONYMOUS: "DNA polymerase I [Escherichia coli]", XP055813469, retrieved from NCBI Database accession no. WP_075861667
DATABASE PROTEIN 8 May 2020 (2020-05-08), ANONYMOUS: "DNA polymerase I [Escherichia coli]", XP055813472, retrieved from NCBI Database accession no. EFN5731753
DATABASE PROTEIN 8 May 2020 (2020-05-08), ANONYMOUS: "DNA polymerase I [Escherichia coli]", XP055813472, retrieved from NCBI Database accession no. EFN5731753
See also references of EP 4060033A4
DATABASE PROTEIN 21 January 2021 (2021-01-21), ANONYMOUS: "MULTISPECIES: DNA polymerase I [Enterobacteriaceae]", XP055813466, retrieved from NCBI Database accession no. WP_152028965
M. ASTATKE, NG K., GRINDLEY N. D. F., JOYCE C. M.: "A single side chain prevents Escherichia coli DNA polymerase I (Klenow fragment) from incorporating ribonucleotides", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 95, no. 7, 31 March 1998 (1998-03-31), pages 3402 - 3407, XP055242309, ISSN: 0027-8424, DOI: 10.1073/pnas.95.7.3402
M. ASTATKE, NG K., GRINDLEY N. D. F., JOYCE C. M.: "A single side chain prevents Escherichia coli DNA polymerase I (Klenow fragment) from incorporating ribonucleotides", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 95, no. 7, 31 March 1998 (1998-03-31), pages 3402 - 3407, XP055242309, ISSN: 0027-8424, DOI: 10.1073/pnas.95.7.3402
DATABASE PROTEIN 1 June 2019 (2019-06-01), ANONYMOUS: "DNA polymerase I [Escherichia coli]", XP055813469, retrieved from NCBI Database accession no. WP_075861667
DATABASE PROTEIN 1 June 2019 (2019-06-01), ANONYMOUS: "DNA polymerase I [Escherichia coli]", XP055813469, retrieved from NCBI Database accession no. WP_075861667
DATABASE PROTEIN 8 May 2020 (2020-05-08), ANONYMOUS: "DNA polymerase I [Escherichia coli]", XP055813472, retrieved from NCBI Database accession no. EFN5731753
DATABASE PROTEIN 8 May 2020 (2020-05-08), ANONYMOUS: "DNA polymerase I [Escherichia coli]", XP055813472, retrieved from NCBI Database accession no. EFN5731753
See also references of EP 4060033A4
Attorney, Agent or Firm:
DHC IP ATTORNEYS (CN)
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