Title:
MODIFIED NICOTINAMIDE PHOSPHORIBOSYLTRANSFERASE
Document Type and Number:
WIPO Patent Application WO/2022/202952
Kind Code:
A1
Abstract:
The present invention pertains to a modified (mutant) nicotinamide phosphoribosyltransferase and a method for producing nicotinamide mononucleotide using the same. The modified nicotinamide phosphoribosyltransferase (Nampt) according to the present invention contains an amino acid sequence shown in SEQ ID NO: 1 and/or an amino acid sequence shown in SEQ ID NO: 2 and has an improved activity compared with wild-type Nampt. (a) SEQ ID NO: 1: S-[V/I]-P-A-X1-X2-H-S-[T/V/I]-[M/V/I]-X3; and (b) SEQ ID NO: 2: X4-[S/I]-D-X5 [wherein X1 to X5 are as defined in the description].
Inventors:
HAYASHI TAKAHIRO (JP)
AKIYAMA TAKANORI (JP)
AKIYAMA TAKANORI (JP)
Application Number:
PCT/JP2022/013776
Publication Date:
September 29, 2022
Filing Date:
March 24, 2022
Export Citation:
Assignee:
MITSUBISHI CHEM CORP (JP)
International Classes:
C07K19/00; C12N9/00; C12N9/10; C12N9/12; C12N15/52; C12N15/54; C12N15/62; C12P11/00; C12P13/02; C12P21/02
Domestic Patent References:
| WO2019065876A1 | 2019-04-04 | |||
| WO2020129997A1 | 2020-06-25 | |||
| WO2019065876A1 | 2019-04-04 | |||
| WO2020129997A1 | 2020-06-25 |
Foreign References:
| JP2019525760A | 2019-09-12 |
Other References:
GARTEN ET AL.: "Physiological and pathophysiological roles of NAMPT and NAD metabolism", NATURE REVIEWS ENDOCRINOLOGY, vol. 11, 2015, pages 535 - 546
MARINESCU ET AL.: "β-nicotinamide mononucleotide (NMN) production in Escherichia coli", SCIENTIFIC REPORTS, vol. 8, 2018, XP009528352, DOI: 10.1038/s41598-018-30792-0
SHOJI ET AL.: "Metabolic design for selective production of nicotinamide mononucleotide from glucose and nicotinamide", METAB ENG, vol. S1096-7176, no. 20, 18 November 2020 (2020-11-18), pages 30176 - 2
GARDELL ET AL.: "Boosting NAD+ with a small molecule that activates NAMPT", NAT COMMUN, vol. 10, no. 1, 19 July 2019 (2019-07-19), pages 3241
BLACK ET AL.: "Engineering a nicotinamide mononucleotide redox cofactor system for biocatalysis", NAT CHEM BIOL., vol. 16, no. 1, January 2020 (2020-01-01), pages 87 - 94, XP036965707, DOI: 10.1038/s41589-019-0402-7
BIOCHEMISTRY, vol. 47, 2008, pages 11086 - 11096
"Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
MARINESCU ET AL.: "β-nicotinamide mononucleotide (NMN) production in Escherichia coli", SCIENTIFIC REPORTS, vol. 8, 2018, XP009528352, DOI: 10.1038/s41598-018-30792-0
SHOJI ET AL.: "Metabolic design for selective production of nicotinamide mononucleotide from glucose and nicotinamide", METAB ENG, vol. S1096-7176, no. 20, 18 November 2020 (2020-11-18), pages 30176 - 2
GARDELL ET AL.: "Boosting NAD+ with a small molecule that activates NAMPT", NAT COMMUN, vol. 10, no. 1, 19 July 2019 (2019-07-19), pages 3241
BLACK ET AL.: "Engineering a nicotinamide mononucleotide redox cofactor system for biocatalysis", NAT CHEM BIOL., vol. 16, no. 1, January 2020 (2020-01-01), pages 87 - 94, XP036965707, DOI: 10.1038/s41589-019-0402-7
BIOCHEMISTRY, vol. 47, 2008, pages 11086 - 11096
"Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
Attorney, Agent or Firm:
UMEDA Shinsuke et al. (JP)
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