Title:
MODIFIED NUCLEOTIDES AND REAL-TIME POLYMERASE REACTION USING SAME
Document Type and Number:
WIPO Patent Application WO/2012/023675
Kind Code:
A1
Abstract:
The present invention relates to modified nucleotides and to a real-time polymerase reaction using same, and particularly, to nucleotides linked to a fluorescent substance, to a composition containing same for a real-time polymerase reaction, to an analysis kit, and to a method for analysis. According to the present invention, by chemically modifying nucleotides, which are a polymerase reaction substrate, so as to impart the function of fluorescent signal generation in addition to the substrate function, there is no need to make a probe, thus providing cost effectiveness; and the nucleotides can easily be applied to various real-time polymerase reactions such as PCR, RCA, and isothermal polymer reactions, so as to provide significantly higher analysis performance than that of existing methods.
Inventors:
AHN DAE-RO (KR)
Application Number:
PCT/KR2011/000581
Publication Date:
February 23, 2012
Filing Date:
January 27, 2011
Export Citation:
Assignee:
KOREA INST SCI & TECH (KR)
AHN DAE-RO (KR)
AHN DAE-RO (KR)
International Classes:
C12Q1/68; C07H21/00; C12N15/11; G01N33/52
Foreign References:
| US7125671B2 | 2006-10-24 | |||
| US7405281B2 | 2008-07-29 |
Other References:
KUMAR, S. ET AL.: "Terminal phosphate labeled nucleotides: synthesis, applications, and linker effect on incorporation by DNA polymerases", NUCLEOSIDES,NUCLEOTIDES,AND NUCLEIC ACIDS., vol. 24, no. 5-7, 2005, pages 401 - 408
SOOD, A. ET AL.: "Terminal phosphate-labeled nucleotides with improved substrate properties for homogeneous nucleic acid assays", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 127, no. 8, 2 March 2005 (2005-03-02), pages 2394 - 2395
SOOD, A. ET AL.: "Terminal phosphate-labeled nucleotides with improved substrate properties for homogeneous nucleic acid assays", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 127, no. 8, 2 March 2005 (2005-03-02), pages 2394 - 2395
Attorney, Agent or Firm:
YOU ME PATENT & LAW FIRM (649-10Yoksam-dong, Kangnam-ku, Seoul 135-080, KR)
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Claims: