Title:
MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME
Document Type and Number:
WIPO Patent Application WO/2011/106766
Kind Code:
A3
Abstract:
Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.
Inventors:
DAVIDSON JOHN F (US)
HINZ WOLFGANG (US)
ROTHBERG JONATHAN (US)
WHITAKER RICHARD (US)
HINZ WOLFGANG (US)
ROTHBERG JONATHAN (US)
WHITAKER RICHARD (US)
Application Number:
PCT/US2011/026450
Publication Date:
April 26, 2012
Filing Date:
February 28, 2011
Export Citation:
Assignee:
LIFE TECHNOLOGIES CORP (US)
DAVIDSON JOHN F (US)
HINZ WOLFGANG (US)
ROTHBERG JONATHAN (US)
WHITAKER RICHARD (US)
DAVIDSON JOHN F (US)
HINZ WOLFGANG (US)
ROTHBERG JONATHAN (US)
WHITAKER RICHARD (US)
International Classes:
C12Q1/68; C12N9/12; C12Q1/48
Domestic Patent References:
| WO2011106634A2 | 2011-09-01 |
Foreign References:
| US20060240439A1 | 2006-10-26 | |||
| US20060199214A1 | 2006-09-07 | |||
| US20030138834A1 | 2003-07-24 |
Other References:
ZHANG, L. ET AL.: "Mutagenic nucleotide incorporation and hindered translocation by a food carcinogen C8-dG adduct in Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): modeling and dynamics studies", NUCLEIC ACID RESEARCH, vol. 34, no. 11, 4 July 2006 (2006-07-04), pages 3326 - 3337
ANDERSON, J. P. ET AL.: "Incorporation of reporter-labeled nucleotides by DNA polymerases", BIOTECHNIQUES, vol. 38, no. 2, February 2005 (2005-02-01), pages 257 - 264
ANDERSON, J. P. ET AL.: "Incorporation of reporter-labeled nucleotides by DNA polymerases", BIOTECHNIQUES, vol. 38, no. 2, February 2005 (2005-02-01), pages 257 - 264
Attorney, Agent or Firm:
DOSHI, Nishita et al. (Attention: IP Department5791 Van Allen Wa, Carlsbad CA, US)
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