Title:
MONOCLONAL ANTIBODY OF N ANTIGEN OF SARS-COV-2, DETECTION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/027703
Kind Code:
A1
Abstract:
An immune detection method on the basis of an antibody that specifically binds to an N antigen of SARS-CoV-2, which is used for the diagnosis of COVID-19. The method comprises marking a capture antibody combination that specifically binds to an N antigen having a detection signal molecule, and detecting the antibody. By means of the method, the presence of an antigen is detected by specifically binding an antibody to an antigen; and the capture antibody is a murine monoclonal antibody combination, which is composed of M1, M2 and M3, the detection antibody is a murine monoclonal antibody M4, and the detection signal molecule can be a colloidal gold or fluorescein. The detection method can be used to specifically detect the N antigen of SARS-CoV-2 in human samples such as from nasopharyngeal swabs, urine and serum, so as to diagnose COVID-19 early, which can be applied to rapid screening and diagnosis in hospitals and on-site.
Inventors:
WU YUZHANG (CN)
Application Number:
PCT/CN2020/108323
Publication Date:
February 10, 2022
Filing Date:
August 11, 2020
Export Citation:
Assignee:
THIRD MILITARY MEDICAL UNIV (CN)
International Classes:
C07K16/10; C07K14/165; C12N15/50; C12N15/70
Foreign References:
| CN111153991A | 2020-05-15 | |||
| CN111269313A | 2020-06-12 | |||
| CN111217917A | 2020-06-02 | |||
| CN111440229A | 2020-07-24 | |||
| CN111337668A | 2020-06-26 |
Other References:
LI MAOHUA, JIN RONGHUA, PENG YA, WANG CUIYAN, REN WENLIN, LV FUDONG, GONG SITAO, FANG FENG, WANG QIANYUN, LI JIANLI, SHEN TONG, SU: "Generation of antibodies against COVID-19 virus for development of diagnostic tools", MEDRXIV, 27 February 2020 (2020-02-27), pages 1 - 17, XP009531584, DOI: 10.1101/2020.02.20.20025999
BURBELO PETER D, RIEDO FRANCIS X, MORISHIMA CHIHIRO, RAWLINGS STEPHEN, SMITH DAVEY, DAS SANCHITA, STRICH JEFFREY R, CHERTOW DANIEL: "Sensitivity in Detection of Antibodies to Nucleocapsid and Spike Proteins of Severe Acute Respiratory Syndrome Coronavirus 2 in Patients With Coronavirus Disease 2019", JOURNAL OF INFECTIOUS DISEASES, UNIVERSITY OF CHICAGO PRESS, US, vol. 222, no. 2, 29 June 2020 (2020-06-29), US , pages 206 - 213, XP055818914, ISSN: 0022-1899, DOI: 10.1093/infdis/jiaa273
BURBELO PETER D, RIEDO FRANCIS X, MORISHIMA CHIHIRO, RAWLINGS STEPHEN, SMITH DAVEY, DAS SANCHITA, STRICH JEFFREY R, CHERTOW DANIEL: "Sensitivity in Detection of Antibodies to Nucleocapsid and Spike Proteins of Severe Acute Respiratory Syndrome Coronavirus 2 in Patients With Coronavirus Disease 2019", JOURNAL OF INFECTIOUS DISEASES, UNIVERSITY OF CHICAGO PRESS, US, vol. 222, no. 2, 29 June 2020 (2020-06-29), US , pages 206 - 213, XP055818914, ISSN: 0022-1899, DOI: 10.1093/infdis/jiaa273
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (CN)
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