Title:
MONOCLONAL ANTIBODY AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2008/032712
Kind Code:
A1
Abstract:
Disclosed is an antibody which does not recognize the full length of OPN but recognizes
only the N-half of OPN. Also disclosed is a use application of the antibody. Specifically
disclosed is a monoclonal antibody which recognizes a protein or polypeptide
having an amino acid sequence: YGLR (SEQ ID NO:1) at the C-terminus but does not
substantially recognize a protein or polypeptide having an amino acid sequence:
YGLR at any region other than the C-terminus. Further disclosed are a method for
the determination of the N-half of OPN, a method for the diagnosis of a disease
associated with the N-half of OPN, a method for the determination of the severity
of the disease, and a therapeutic method for the disease, each utilizing the antibody.
Inventors:
KOBAYASHI HIROKO (JP)
HASEGAWA MASAHIRO (JP)
SEITO TSUTOMU (JP)
HASEGAWA MASAHIRO (JP)
SEITO TSUTOMU (JP)
Application Number:
PCT/JP2007/067666
Publication Date:
March 20, 2008
Filing Date:
September 11, 2007
Export Citation:
Assignee:
IMMUNO BIOLOGICAL LAB CO LTD (JP)
KOBAYASHI HIROKO (JP)
HASEGAWA MASAHIRO (JP)
SEITO TSUTOMU (JP)
KOBAYASHI HIROKO (JP)
HASEGAWA MASAHIRO (JP)
SEITO TSUTOMU (JP)
International Classes:
C12N15/00; A61K39/395; A61P37/02; C07K16/18; C12N15/09; G01N33/53; C12P21/08
Domestic Patent References:
| WO2002081522A1 | 2002-10-17 |
Other References:
KON S. ET AL.: "Mapping of functional epitopes of osteopontin by monoclonal antibodies raised against defined internal sequences", J. CELL BIOCHEM., vol. 84, no. 2, 2002, pages 420 - 432, XP002961001
YOKOSAKI Y. ET AL.: "The integrin alpha9beta1 binds to a novel recognition sequence (SVVYGLR) in the thrombin-cleaved amino-terminal fragment of osteopontin", J. BIOL. CHEM., vol. 274, no. 51, 1999, pages 36328 - 36334, XP002953833
YAMAMOTO N. ET AL.: "Essential role of the cryptic epitope SLAYGLR within osteopontin in a murine model of rheumatoid arthritis", J. CLIN. INVEST., vol. 112, 2003, pages 181 - 188, XP003021585
HOTTA H. ET AL.: "Detection of various epitopes of murine osteopontin by monoclonal antibodies", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 257, no. 1, 1999, pages 6 - 11, XP002286858
B. F. ERLANGER ET AL., J. BIOL. CHEM., vol. 234, 1954, pages 1090 - 1094
A. E. KARU ET AL., J. AGRIC. FOOD CHEM., vol. 42, 1994, pages 301 - 309
E. ENGVALL ET AL., METHODS IN ENZYMOL., vol. 70, 1980, pages 419 - 439
"Hybridoma method and monoclonal antibody", 5 March 1982, R&D PLANNING INC., pages: 30 - 53
"Human Osteopontin Assay Kit (L)-IBL", IMMUNO-BIOLOGICAL LABORATORIES CO., LTD.
J. IMMUNOL., vol. 146, pages 3721 - 3728
AIST TSUKUBA CENTRAL 6, 11 July 2006 (2006-07-11), pages 305 - 8566
"Human Osteopontin Assay Kit (L) - IBL", IMMUNO-BIOLOGICAL LABORATORIES CO., LTD.
YOKOSAKI Y. ET AL.: "The integrin alpha9beta1 binds to a novel recognition sequence (SVVYGLR) in the thrombin-cleaved amino-terminal fragment of osteopontin", J. BIOL. CHEM., vol. 274, no. 51, 1999, pages 36328 - 36334, XP002953833
YAMAMOTO N. ET AL.: "Essential role of the cryptic epitope SLAYGLR within osteopontin in a murine model of rheumatoid arthritis", J. CLIN. INVEST., vol. 112, 2003, pages 181 - 188, XP003021585
HOTTA H. ET AL.: "Detection of various epitopes of murine osteopontin by monoclonal antibodies", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 257, no. 1, 1999, pages 6 - 11, XP002286858
B. F. ERLANGER ET AL., J. BIOL. CHEM., vol. 234, 1954, pages 1090 - 1094
A. E. KARU ET AL., J. AGRIC. FOOD CHEM., vol. 42, 1994, pages 301 - 309
E. ENGVALL ET AL., METHODS IN ENZYMOL., vol. 70, 1980, pages 419 - 439
"Hybridoma method and monoclonal antibody", 5 March 1982, R&D PLANNING INC., pages: 30 - 53
"Human Osteopontin Assay Kit (L)-IBL", IMMUNO-BIOLOGICAL LABORATORIES CO., LTD.
J. IMMUNOL., vol. 146, pages 3721 - 3728
AIST TSUKUBA CENTRAL 6, 11 July 2006 (2006-07-11), pages 305 - 8566
"Human Osteopontin Assay Kit (L) - IBL", IMMUNO-BIOLOGICAL LABORATORIES CO., LTD.
Attorney, Agent or Firm:
ONO, Nobuo (1-13-1 Kandaizumi-ch, Chiyoda-ku Tokyo 24, JP)
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