Title:
MONONUCLEOSOME AND PROCESS FOR PRODUCING THE SAME, METHOD OF ASSAYING ANTIBODY SPECIFIC TO NUCLEOSOME, METHOD OF DIAGNOSING AUTOIMMUNE DISEASE, PROCESS FOR PRODUCING NUCLEOSOME DNA, DNA PLATE, PROCESS FOR PRODUCING DNA PLATE AND METHOD OF ASSAYING ANTI-DNA ANTIBODY
Document Type and Number:
WIPO Patent Application WO2002076377
Kind Code:
A3
Abstract:
It is intended to provide a process whereby a highly pure mononucleosome can be conveniently and efficiently produced. Namely, a process for producing a mononucleosome involving: the step of capturing and collecting a nucleosome contained in a sample by using an antibody specific to the nucleosome; the step of dissociating and collecting the thus captured nucleosome from the antibody; and the step of isolating and purifying a mononucleosome from the collected nucleosome depending on molecular weight.
Inventors:
KANAI YOSHIYUKI (JP)
Application Number:
PCT/JP2002/002664
Publication Date:
November 21, 2002
Filing Date:
March 20, 2002
Export Citation:
Assignee:
KANAI YOSHIYUKI (JP)
CT FOR ADVANCED SCIENCE & TECH (JP)
CT FOR ADVANCED SCIENCE & TECH (JP)
International Classes:
C07K16/44; G01N33/564; G01N33/68; (IPC1-7): C12N15/09; C12P19/34; C12Q1/68; G01N27/447; G01N30/88; G01N33/53
Domestic Patent References:
| WO1999047924A1 | 1999-09-23 |
Other References:
CHIU M.L. ET AL.: "Absence of histone H2B in nucleosomes containing histone TH2B and interaction of immunoglobulin with nucleosomes", ARCH. BIOCHEM. BIOPHYS., vol. 244, no. 1, January 1986 (1986-01-01), pages 42 - 49, XP002953859
KOUTOUZOV S. ET AL.: "Binding of nucleosomes to a cell surface receptor: redistribution and endocytosis in the presence of lupus antibodies", EUR. J. IMMUNOL., vol. 26, no. 2, February 1996 (1996-02-01), pages 472 - 486, XP002953860
WILLIAMS R.C. ET AL.: "Detection of nucleosome particles in serum and plasma from patients with systemic lupus erythematosus using monoclonal antibody 4H7", J. RHEUMATOL., vol. 28, no. 1, January 2001 (2001-01-01), pages 81 - 94, XP002953861
KUBOTA T. ET AL.: "A monoclonal anti-double stranded DNA antibody from an autoimmune MRL/Mp-1pr/1pr mouse: specificity and idiotype in serum immunoglobulins", IMMUNOL. LETT., vol. 14, no. 1, 17 November 1986 (1986-11-17), pages 53 - 58, XP000601123
STEMMER C. ET AL.: "Dual reactivity of several monoclonal anti-nucleosome autoantibodies for double-stranded DNA and a short segment of histone H3", J. BIOL. CHEM., vol. 271, no. 35, 30 August 1996 (1996-08-30), pages 21257 - 21261, XP002953863
MENDELSON E. ET AL.: "Immunofractionation of chromatin regionsassociated with histone H1o", EUR. J. BIOCHEM., vol. 160, no. 2, 15 October 1986 (1986-10-15), pages 253 - 260, XP002953864
KOUTOUZOV S. ET AL.: "Binding of nucleosomes to a cell surface receptor: redistribution and endocytosis in the presence of lupus antibodies", EUR. J. IMMUNOL., vol. 26, no. 2, February 1996 (1996-02-01), pages 472 - 486, XP002953860
WILLIAMS R.C. ET AL.: "Detection of nucleosome particles in serum and plasma from patients with systemic lupus erythematosus using monoclonal antibody 4H7", J. RHEUMATOL., vol. 28, no. 1, January 2001 (2001-01-01), pages 81 - 94, XP002953861
KUBOTA T. ET AL.: "A monoclonal anti-double stranded DNA antibody from an autoimmune MRL/Mp-1pr/1pr mouse: specificity and idiotype in serum immunoglobulins", IMMUNOL. LETT., vol. 14, no. 1, 17 November 1986 (1986-11-17), pages 53 - 58, XP000601123
STEMMER C. ET AL.: "Dual reactivity of several monoclonal anti-nucleosome autoantibodies for double-stranded DNA and a short segment of histone H3", J. BIOL. CHEM., vol. 271, no. 35, 30 August 1996 (1996-08-30), pages 21257 - 21261, XP002953863
MENDELSON E. ET AL.: "Immunofractionation of chromatin regionsassociated with histone H1o", EUR. J. BIOCHEM., vol. 160, no. 2, 15 October 1986 (1986-10-15), pages 253 - 260, XP002953864
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